Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,192,452	(A)6→5	intergenic (‑230/+63)	yehD ← / ← yehE	putative fimbrial‑like adhesin protein/DUF2574 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,192,447	0	A	.	100.0%	249.5 / NA	51	intergenic (‑225/+68)	yehD/yehE	putative fimbrial‑like adhesin protein/DUF2574 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (32/19);  total (32/19)

AGTTGCCGCAATGTAAACACATTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAATAAAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCTGTCGTTACACCTTTG  >  NC_000913/2192300‑2192590                                                                                                                                                    |                                                                                                                                                aGTTGCCGCAATGTAAACACATTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAATaaaa                                                                                                                                              <  1:266808/151‑1 (MQ=255)    tGCCGCAATGTAAACACATTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAATaaaaagc                                                                                                                                           >  2:21880/1‑149 (MQ=255)      ccGCAATGTAAACACATTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAATaaaaagccg                                                                                                                                         >  1:102528/1‑147 (MQ=255)        gCAATGTAAACACATTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGa                                                                                                                                       >  1:547362/1‑150 (MQ=255)           aTGTAAACACATTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATc                                                                                                                                   >  1:170791/1‑151 (MQ=255)             gTAAACACATTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCAt                                                                                                                                 <  1:789218/151‑1 (MQ=255)               aaaCACATTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCAtt                                                                                                                                <  1:98866/150‑1 (MQ=255)                  cacaTTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTtat                                                                                                                             >  1:974133/1‑150 (MQ=255)                   acaTTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTtatat                                                                                                                           <  2:776912/151‑1 (MQ=255)                     aTTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATaa                                                                                                                         <  1:553626/151‑1 (MQ=255)                           ttATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGAt                                                                                                                    <  2:507756/150‑1 (MQ=255)                             aTTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGAttt                                                                                                                  <  2:208485/150‑1 (MQ=255)                               tGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCgg                                                                                                               >  2:985670/1‑151 (MQ=255)                                   ttAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCttt                                                                                                           >  2:347149/1‑151 (MQ=255)                                   ttAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCttt                                                                                                           >  2:811128/1‑151 (MQ=255)                                     aaaaCAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCt                                                                                                         <  2:983236/151‑1 (MQ=255)                                          aaaTTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATg                                                                                                    >  2:226654/1‑151 (MQ=255)                                           aaTTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGc                                                                                                   <  2:212538/151‑1 (MQ=255)                                            aTTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCa                                                                                                  <  1:842675/151‑1 (MQ=255)                                                  cATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTaa                                                                                             >  1:598874/1‑150 (MQ=255)                                                  cATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTaa                                                                                             >  1:214252/1‑150 (MQ=255)                                                           ataAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGtt                                                                                    >  2:568458/1‑150 (MQ=255)                                                              aaTACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGcc                                                                                 >  1:225487/1‑150 (MQ=255)                                                              aaTACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCa                                                                                <  1:722734/151‑1 (MQ=255)                                                                        atatTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGt                                                                      <  2:368419/151‑1 (MQ=255)                                                                        atatTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGt                                                                      >  1:11562/1‑151 (MQ=255)                                                                         tatTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGt                                                                      >  1:10840/1‑150 (MQ=255)                                                                         tatTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGt                                                                      >  1:243178/1‑150 (MQ=255)                                                                          atTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTa                                                                     <  1:575666/150‑1 (MQ=255)                                                                            ttAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCg                                                                  >  2:717507/1‑151 (MQ=255)                                                                             tAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCg                                                                  <  1:292887/150‑1 (MQ=255)                                                                               agagTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGAtt                                                               >  1:593682/1‑151 (MQ=255)                                                                                   tATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAac                                                           >  1:821754/1‑151 (MQ=255)                                                                                    aTTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAac                                                           >  2:585809/1‑150 (MQ=255)                                                                                      tAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAacac                                                         <  2:257619/150‑1 (MQ=255)                                                                                         cTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGAtt                                                     >  1:729215/1‑151 (MQ=255)                                                                                          tATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTc                                                    >  2:438261/1‑151 (MQ=255)                                                                                             ttATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGAc                                                 >  2:141257/1‑151 (MQ=255)                                                                                              tATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGAc                                                 <  1:777374/150‑1 (MQ=255)                                                                                               aTCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGatg                                                <  2:883509/150‑3 (MQ=255)                                                                                               aTCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGC‑TTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGtt                                              >  1:388147/1‑151 (MQ=255)                                                                                                tCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGtt                                              >  2:829802/1‑151 (MQ=255)                                                                                                       tATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATc                                       >  1:35231/1‑151 (MQ=255)                                                                                                              tAATGTAGATTTATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCt                                >  1:681203/1‑151 (MQ=255)                                                                                                                         tATGTAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCAc                     >  1:203324/1‑151 (MQ=255)                                                                                                                             tAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCt                 >  2:657167/1‑151 (MQ=255)                                                                                                                             tAAATGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCt                 >  1:930717/1‑151 (MQ=255)                                                                                                                              aaaTGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCTg                <  2:424234/151‑1 (MQ=255)                                                                                                                               aaTGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCTGt               >  2:957976/1‑151 (MQ=255)                                                                                                                                 tGGTATTGTTGATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCTGTCg             >  2:6694/1‑151 (MQ=255)                                                                                                                                       tgttgATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCTGTCGTTaca        <  1:568470/150‑1 (MQ=255)                                                                                                                                        gttgATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCTGTCGTTacac       <  1:57579/150‑1 (MQ=255)                                                                                                                                          tgATATCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCTGTCGTTACACCtt    >  1:718163/1‑151 (MQ=255)                                                                                                                                             tatCAAT‑AAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCTGTCGTTACACCTTTg  >  2:675807/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                AGTTGCCGCAATGTAAACACATTGTTTTATTGATTTAAAACAAATTAACCATTGCAATATAAATACAAAAAATATTTAAGAGTATTAACTATTTATCGCATCTATCAATTAATGTAGATTTATGTAAATGGTATTGTTGATATCAATAAAAAAGCCGAAATCATTTATATAAATGATTTCGGCTTTCTAAATGCAATTAAAATCAGGTTGCCAAATTAATCGTAGCGATTTAACACGATTCGACGTTTGCTATCGCTACCTGCAACAACCACTTCTGTCGTTACACCTTTG  >  NC_000913/2192300‑2192590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑