Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA44.3% 75.0 / 33.3 61intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (29/5);  new base A (0/27);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 2.02e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAA  >  NC_000913/1824909‑1825203
                                                                                                                                                     |                                                                                                                                                   
gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagagaa                                                                                                                                                   >  1:546710/1‑150 (MQ=255)
gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                                  <  1:271075/151‑1 (MQ=255)
 aCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaa                                                                                                                                                 <  2:918360/151‑1 (MQ=255)
 aCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaa                                                                                                                                                 <  2:782248/151‑1 (MQ=255)
 aCATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                                  >  2:961271/1‑150 (MQ=255)
     tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagta                                                                                                                                              >  1:570191/1‑148 (MQ=255)
      ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaaa                                                                                                                                             >  1:848360/1‑150 (MQ=255)
        aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAcc                                                                                                                                          >  1:11556/1‑151 (MQ=255)
        aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAcc                                                                                                                                          <  1:710536/151‑1 (MQ=255)
        aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAcc                                                                                                                                          >  1:298447/1‑151 (MQ=255)
         cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccg                                                                                                                                         <  1:339121/151‑1 (MQ=255)
                   aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGt                                                                                                                               >  2:592260/1‑151 (MQ=255)
                       ttCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAc                                                                                                                           >  1:27184/1‑151 (MQ=255)
                        tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                          >  2:291531/1‑151 (MQ=255)
                            tcctcccccccGCCCCTTTTTTTTCATTTTTATCGTTCAGGTAACGGTGGTTGTTTAGCGGCACCTGGGGTGATTTACGTTAGGGGTGATCAGGTTTTTTTTACACCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCAcc                                                                                                                          <  2:604167/139‑1 (MQ=255)
                              cTCGCCCCCGCCCCGTTTTTTTAATTTTTATCGTTAGGGTAACCGTGATTGTTTAGCGGCACCTCGGGTGATTTCCTTTGTTGTGATTCAGGTTTTTTTTACAACTGTTGGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCa                                                                                                                       <  2:324322/148‑1 (MQ=255)
                                       ggCCCGTTCTTTTCATTGTTTTCGTTCAGGTACCGGTGGATGGTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                            <  1:909679/150‑1 (MQ=255)
                                           cGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTg                                                                                                       >  1:807536/1‑151 (MQ=255)
                                               cTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                    >  2:568410/1‑150 (MQ=255)
                                                  ttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                   >  2:762654/1‑148 (MQ=255)
                                                  ttCATTGTTATCGTTAAGGTAACCGTGGTTGGTTATCGCAACCGGGGGTGATTTATTTTGGTGGTGATCAGGTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                                 <  2:166200/150‑1 (MQ=255)
                                                   tCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAc                                                                                               >  2:757377/1‑151 (MQ=255)
                                                        gTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                          >  1:860021/1‑151 (MQ=255)
                                                          tATGGTTCAGTTAACCGTGGTTGGTTATGGGAACCTGGGGTGATTTACTTTGGTGGTAATCGGGTTTTTTTTAAACCTGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                           <  2:430762/148‑1 (MQ=255)
                                                             cgttagggaaaCGGTGGATGGTTATCGGAACCTGGGGTGTTTTACGTTAGTGGTAATCAGGTTTTTTTTACACCTGTTTAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                        <  1:716968/139‑1 (MQ=255)
                                                               ttCAGGTACCCGTGGATGTTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACATCTGTTGGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                        <  2:29219/146‑1 (MQ=255)
                                                               ttCAGGTACCCGTGGATGGTTATCGGCAACTCGGGTGATTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                    <  2:127229/150‑1 (MQ=255)
                                                               ttCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCtt                                                                                     >  1:353935/1‑149 (MQ=255)
                                                                  aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                >  1:798347/1‑151 (MQ=255)
                                                                   ggtaccgggggttGGTTACCGGCACCTGGGGTAATTTCCGTTGGTGGTGATCGGTTTTTTTTTACACTTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                <  2:571493/138‑1 (MQ=255)
                                                                      accggTGGATGGTTATCGGCAACTGGGGTTATTTACGTTGGTGGGGACCGGTTTTTTTTAACACTTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                            <  1:512444/147‑1 (MQ=255)
                                                                      aacgggggATGTTTTGCGGCAACTGGGGTGATTTGCGTTAGTGGTGATCGGTTTTTTTTTACATCTGTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                            <  1:396953/145‑1 (MQ=255)
                                                                      aaCCTTGGTTGGTTTCCGGCACCTGGGGTTATTTACTTTAGTGTTGACCAGGTTTTTTTTACAACTGTTTAAGAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGt                                                                                 <  1:22658/146‑1 (MQ=255)
                                                                          ggtgactttactCGGAAACGGGGGTAATTTCTTTTAGTGGTGATCTGGTTTTTTTAACATTTTTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttttt                                                                             <  2:835596/135‑1 (MQ=255)
                                                                           tGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGAAGTGGACAAGACCGGCGGACTTACGTTTTTTgg                                                                          >  1:895295/1‑148 (MQ=255)
                                                                                ggTTATCGGCAACTGGGGTGATTTACGTTGGGGGTGACCGGGCTTTTTTTACAACTTTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg                                                                   <  1:807495/150‑1 (MQ=255)
                                                                                  ttATCGGAAACTGGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTTTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGa                                                                      <  1:478870/145‑1 (MQ=255)
                                                                                      cGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                             >  1:428779/1‑150 (MQ=255)
                                                                                       ggCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                            <  1:77628/150‑1 (MQ=255)
                                                                                       ggCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                            >  1:494558/1‑150 (MQ=255)
                                                                                       gccAACTGGGTTGATTTAGGTTGGTGGTGATCGGGTTTTTTTTACAACTTTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                           <  2:450954/149‑1 (MQ=255)
                                                                                          acctcgtgttttttACTTTGGTGGTGATCAGGTTTTTTTTACATTTTTTAGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                         <  2:392177/139‑1 (MQ=255)
                                                                                          aaCTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                          <  1:250321/149‑1 (MQ=255)
                                                                                          aaCTCGGGTGATTTACGTTAGGGGTGATCAGGTTTTTTTTACAACTTTTAGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca                                                        <  1:738696/151‑1 (MQ=255)
                                                                                           aCTCGGGTGATTTACGTTAGGGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAAATTATTcagc                                                         >  2:923620/1‑149 (MQ=255)
                                                                                              cGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                     >  2:696948/1‑150 (MQ=255)
                                                                                               ggttGATTTATGTTAGGGGTGATCAGTTTTTTTTTACAACTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                     <  2:574521/146‑1 (MQ=255)
                                                                                                 gTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                   >  1:132064/1‑149 (MQ=255)
                                                                                                  tGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAgg                                                >  2:181077/1‑151 (MQ=255)
                                                                                                      ttACTTTGGTGGTGACCAGGTTTTTTTTAAACCTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAt                                             <  1:141825/150‑1 (MQ=255)
                                                                                                       tACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAATTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                           >  2:791857/1‑151 (MQ=255)
                                                                                                            tAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt                                      >  1:521191/1‑151 (MQ=255)
                                                                                                                ggtgATCAGTCTTTTTTTACAACTGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg                                  <  1:873893/151‑1 (MQ=255)
                                                                                                                  ggtaacgagttttttttAACACTGTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg                                  <  2:221662/141‑1 (MQ=255)
                                                                                                                   tgaccagtttttttttACACCTGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                    <  2:721843/138‑1 (MQ=255)
                                                                                                                    aTCAGTTTTTTTTAACATCTTTTGGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                                <  2:272163/149‑1 (MQ=255)
                                                                                                                    aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCg                               >  1:628288/1‑150 (MQ=255)
                                                                                                                    aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGc                              >  2:35407/1‑151 (MQ=255)
                                                                                                                         gtttttttttACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGc                              <  2:192774/144‑1 (MQ=255)
                                                                                                                         ggttttttttACAACTGTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                           <  1:899862/147‑1 (MQ=255)
                                                                                                                               tttaACACCTGTTGGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAc                         <  2:941251/146‑1 (MQ=255)
                                                                                                                                 ttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                    >  1:268483/1‑149 (MQ=255)
                                                                                                                                    cAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                  >  2:49614/1‑148 (MQ=255)
                                                                                                                                     aaCTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAg               <  1:762672/150‑1 (MQ=255)
                                                                                                                                       cTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg            >  1:530281/1‑151 (MQ=255)
                                                                                                                                        tGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc           >  1:928985/1‑151 (MQ=255)
                                                                                                                                            ttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtc        >  2:829203/1‑151 (MQ=255)
                                                                                                                                            tggagaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt         <  1:961289/148‑1 (MQ=255)
                                                                                                                                             tGAGAAGAGAAAGGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtc        >  1:439987/1‑150 (MQ=255)
                                                                                                                                                  agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa   >  1:755715/1‑150 (MQ=255)
                                                                                                                                                  agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa   >  1:372757/1‑150 (MQ=255)
                                                                                                                                                  aaagaaaagaaaCGCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCGGTCAGACGCTTCTCaaaa  <  2:312178/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAA  >  NC_000913/1824909‑1825203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: