Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	213,341	0	A	G	44.1%	84.2 / 25.1	68	G337G (GGA→GGG)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (7/31);  new base G (30/0);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 7.44e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTG  >  NC_000913/213191‑213488                                                                                                                                                       |                                                                                                                                                      aGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGa                                                                                                                                                       <  2:967421/151‑1 (MQ=255)   aTGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAg                                                                                                                                                     <  2:659601/151‑1 (MQ=255)    tGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAg                                                                                                                                                     >  1:112747/1‑150 (MQ=255)     gCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAgtg                                                                                                                                                   <  1:951575/151‑1 (MQ=255)         cACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAggggac                                                                                                                                                >  2:93479/1‑145 (MQ=255)              tGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGCAGTGGCTTCAACCGCTGGAATTACCGGAGGGGCGGGGGAgtgtcgcgactc                                                                                                                                          >  2:833168/1‑143 (MQ=255)                 ttACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAAt                                                                                                                                        <  1:324897/150‑1 (MQ=255)                           gcgcGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACAGTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGa                                                                                                                            <  1:236195/151‑1 (MQ=255)                                tttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGAGGGCAACCTAATGC‑AGgggggg                                                                                                                             >  1:329305/1‑143 (MQ=255)                                 ttGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGACAGCTTAAAGC‑gggggggg                                                                                                                             >  2:401618/1‑142 (MQ=255)                                     aaTCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGC‑GGGGGGCGATATTTGccc                                                                                                                   >  1:966408/1‑150 (MQ=255)                                       tCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGCGGGGAGGGGAAAGCTTAATTC‑GGGGGGCGGTATTTGcccccc                                                                                                                >  2:275172/1‑151 (MQ=255)                                       tCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCGGGGGAGGGTGCCGCTTTATGG‑GGGGGGGGAGATTCGcccccc                                                                                                                >  2:241856/1‑151 (MQ=255)                                              cTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCCTAATTT‑GGGAGGCGGTATTCTCCCTCcccg                                                                                                             >  1:884266/1‑147 (MQ=255)                                                   aGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACCGCTTAAAACGGGGGGGGGATATT‑GCACTCCGCG‑GGcgg                                                                                                       >  2:685537/1‑145 (MQ=255)                                                    gTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGaa                                                                                                  <  2:885434/151‑1 (MQ=255)                                                     tCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCCTAATGC‑GGGGGGCGGGATTTGcccccc                                                                                                                >  2:860311/1‑137 (MQ=255)                                                     tCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGC‑GGGGGGGGATATTCGCCCTCCGCG‑TGCAg                                                                                                       >  1:87110/1‑145 (MQ=255)                                                      cGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTAATGC‑GGGGGGGGGTATTTGCCCCCCGCG‑GGCAgaagaa                                                                                                  >  1:342624/1‑149 (MQ=255)                                                       gCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGC‑GGGAGGCGATATTTGCCCTCCCCG‑TGCAGACGaa                                                                                                  >  1:743246/1‑148 (MQ=255)                                                        cAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATGC‑GGGGGGCGGTATTCGCCCTCCGCG‑TGCAGACGAAGCgg                                                                                              >  1:504227/1‑151 (MQ=255)                                                          aCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCGTTATGA‑GGGGGGGGAAATTCGCCCTCCCCG‑GGCAGACGaa                                                                                                  >  2:930904/1‑145 (MQ=255)                                                          aCTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTc                                                                                            <  2:259094/151‑1 (MQ=255)                                                           cTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGC‑GGGGGGGGATATTCGCCCTCCGCC‑TGCAGACGAAGCgggc                                                                                            >  1:422403/1‑148 (MQ=255)                                                            tgtgGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGAGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGAACAACTTAAAAC‑AGGGGGCGATATTTACCCTCCCCA‑GGCAGACggaagagtt                                                                                            >  1:863700/1‑141 (MQ=255)                                                             gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCa                                                                                           <  2:228374/149‑1 (MQ=255)                                                             gtggtgGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAg                                                                                          <  2:700783/150‑1 (MQ=255)                                                              tggtggATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGGACAGCTTAATGC‑GGGGGGCGGTATTTGCCCTCCCCG‑TGCAGACGAAACggg                                                                                             >  1:956589/1‑145 (MQ=255)                                                                   gATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAgcgtgcgt                                                                                   <  1:145962/151‑1 (MQ=255)                                                                      tAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGttt                                                                                 <  2:333344/150‑1 (MQ=255)                                                                           ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATTC‑GGGAGGCGGTATTTGCCCCCCCCG‑GGCCGACGAAGGGGGCAGCGGGCGGTTTAAAg                                                                            >  1:905837/1‑150 (MQ=255)                                                                           ccGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAg                                                                            <  2:30125/150‑1 (MQ=255)                                                                               cACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGcc                                                                        <  2:776434/150‑1 (MQ=255)                                                                                       aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGTGTACAGCTTAATGC‑GGGGGGGGGTATTTCCCCCCCGCG‑TGCAGACGAAGCGGGCAGCGTGCGTTTCAAAACGCCCGGGtt                                                                  >  1:492621/1‑148 (MQ=255)                                                                                       aaaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGGTGGGGAGGGGACAGCCTAATGC‑GGGGGGGGGAATTTGCCCTCCGCG‑GGCCGACGAAGCGGTCAGCGGGGGGTTTAAAACGcc                                                                        >  2:602551/1‑142 (MQ=255)                                                                                        aaGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgctg                                                              <  2:938757/151‑1 (MQ=255)                                                                                         aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGGAAAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCCGACGAAGCGGGCAGCGGGCGTTTTAAAGCGCCAGGGtt                                                                  >  1:509582/1‑146 (MQ=255)                                                                                         aGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATtgctgc                                                             <  1:472698/151‑1 (MQ=255)                                                                                            gAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTTATGG‑GGGGGGGGATATTTGCCCCCCCCG‑TGGCGACGAAGCGGGCAGCGGGCGTTTCAAAACGCCCGGGTtgctg                                                              >  2:871745/1‑147 (MQ=255)                                                                                             aaaaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCCTTATGC‑GGGGGGGGAAATTCGCCCCCCCCG‑CGGCGGAGAAGCGGGCCGCGTGGGGTTCAAAAAGCCAGGGTTTCTGCata                                                          >  2:748672/1‑150 (MQ=255)                                                                                               aaCATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGTACAGCTTAATGC‑GGGGGGCGATAATCGCCCCCCCCG‑TGCAGACGAAACGGGCAGCGTGCGGTTTAAAACGCCAGGATTTCTGCATAtt                                                        >  1:932979/1‑150 (MQ=255)                                                                                                  aTTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCg                                                    <  1:445833/151‑1 (MQ=255)                                                                                                   ttGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAGCTTAATGC‑GGGGGGCGATATTCCCCCTCCGCG‑GGCGGACGAAGCGGGCAGCGGGCGGTTCAAAACGCCCGGATTGCTGCATATTGTCgg                                                   >  2:231181/1‑151 (MQ=255)                                                                                                   ttGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCg                                                    <  1:280473/150‑1 (MQ=255)                                                                                                     gtgCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGc                                                 <  2:334052/151‑1 (MQ=255)                                                                                                         cGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTaa                                             <  2:435471/151‑1 (MQ=255)                                                                                                          gTGGCAGACGTGGCTTCAACCGCTGTAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAc                                            <  2:777698/151‑1 (MQ=255)                                                                                                          gTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGAGGACACCTTCAAGC‑GGGGGGCGAGATTTCCCCGCCTCGCTGCCG‑CGGAGGGGGCAACGGGCGTTTCCAAGCGCCAGGACTTCTGCAAATTTTCGGGCGtga                                             >  1:285352/1‑148 (MQ=255)                                                                                                            ggCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcg                                           <  1:438693/150‑1 (MQ=255)                                                                                                             gCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggc                                         <  1:394678/151‑1 (MQ=255)                                                                                                              cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAAcggcg                                        <  1:527386/151‑1 (MQ=255)                                                                                                              cAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAAGGGACAGCTTAAAGC‑GGGGGGCGATATTCCCCCTCCCCG‑GGCCGACGAAGCGGGCAGCGGGGGGTTCCAAACGCCAGGAATGCTGCCTATTGTCGGGCGGaa                                             >  2:624949/1‑146 (MQ=255)                                                                                                               aGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGTGGACAGCTTTATGC‑GGGGGGGGGTATTCGCCCCCCGCG‑TGCAGACGAAACGGGCAGCGGGGGGTTCCAAACGCCAGGATTGGTGCCTATTGTCGGGGGGAAcggggg                                       >  1:643482/1‑151 (MQ=255)                                                                                                               aGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAACTTAATGC‑GGGAGGGGGTATTTGCCCCCCCCG‑TGCAGACGAAGCGGGCAGCGTGCGGTTTAAAGCGCCAGGATTGCTGCATATTGTCGGGGGTAAcggcgg                                       >  1:629184/1‑151 (MQ=255)                                                                                                                  cGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGGGGACAGCTTAATGC‑GGGGGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGGCAGCGGGCGTTTTAAAACGCCAGGATTGCTGCATATTTTCGGGGGTAAcggcgg                                       >  1:234161/1‑148 (MQ=255)                                                                                                                         tCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCt                              <  2:222942/150‑1 (MQ=255)                                                                                                                              cGCTGGAATTACCGGCGGGGCTGGGGAGGGTACAACTTAATTC‑GGGGGGCGATATTTGCCCTCCGCG‑GGCAGACGAAGCGGTCAGCGTGCGGTTCAAAACGCCCGGATTGGTGCATATTTTCGGGCGGAACGGCGGGCGTAAGcgcaa                           >  2:427654/1‑144 (MQ=255)                                                                                                                               gCTGGAATTACCGGCGGGGGTGGGGAGGGTACAGCTTAATGC‑GGGAGGGGGTATTTGCCCTCCGCG‑TGCAGACGAAGCGGGCAGCGGGCGGTTTAAAGCGCCAGGGTTTCTGCATATTGTCGGGCGTAAAGGCGGGCGTAAGGTAAAGaa                        >  1:429461/1‑150 (MQ=255)                                                                                                                                cTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTTATGC‑GGGGGGCGATATTTGCCCCCCGCG‑GGCAGACGAAGCGGTCCGCGGGGGGTTCAAAAAGCCAGGATTGCTGCATATTTTCGGGGGGAACGGCGGGCGTAAGGTAAAGaaa                       >  2:771567/1‑150 (MQ=255)                                                                                                                                 tGGAATTACCGGCGGGGCTGGGGAGTGTACAGCTTAATGC‑GGGGGGCGGTATTCGCCCCCCGCG‑TGCAGACGAAGCGGTCAGCGGGCGTTTCAAAGCGCCAGGGTTGCTGCCTATTTTCGGGCGGAACGGCGGGCGGAAGCTaaaaaaaa                      >  1:731109/1‑150 (MQ=255)                                                                                                                                   gAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCt                   <  2:279106/151‑1 (MQ=255)                                                                                                                                        aCCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCa               <  1:614229/150‑1 (MQ=255)                                                                                                                                          cggcggGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAaga            <  2:148988/151‑1 (MQ=255)                                                                                                                                           ggcggGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAagag           <  2:463736/151‑1 (MQ=255)                                                                                                                                           ggcggGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAag             >  1:908106/1‑149 (MQ=255)                                                                                                                                               gggCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg       <  2:223529/151‑1 (MQ=255)                                                                                                                                               gggCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTgg       <  2:161896/151‑1 (MQ=255)                                                                                                                                                 gCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGGGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGc     <  2:702905/151‑1 (MQ=255)                                                                                                                                                    gggAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTg  <  2:112745/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                      AGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAGCTTAATGC‑GGGAGGCGATATTCGCCCTCCGCG‑TGCAGACGAAGCGGTCAGCGTGCGTTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTG  >  NC_000913/213191‑213488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑