| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 718,690 | 0 | T | G | 43.8% | 74.8 / 14.3 | 48 | T591P (ACC→CCC) | speF | ornithine decarboxylase isozyme, inducible |
| Reads supporting (aligned to +/- strand): ref base T (5/22); new base G (21/0); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.22e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTCAGCAGG > NC_000913/718545‑718838 | tCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAAAAATGCATTTTCTGACAC‑AAATGACGCAGGGGATaa > 2:827640/1‑150 (MQ=255) gcgTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGGATACAAAACATGGATTTTCTGACAC‑AACTGACGCAGGGGATAAACGGc > 1:823015/1‑150 (MQ=255) aGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGctc < 2:301247/151‑1 (MQ=255) aGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGct < 1:679719/150‑1 (MQ=255) ggCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTg < 1:192020/150‑1 (MQ=255) ggCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTg < 1:901649/150‑1 (MQ=255) ggCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGt < 1:718548/151‑1 (MQ=255) gCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGtt < 2:582659/151‑1 (MQ=255) cttcttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCCTACAAAAAATGCATTTTCTTACAC‑AACAGACGCAAGGGATAAACGGCGTAGCGGTCTTCCTTCTGttt > 1:299259/1‑151 (MQ=255) tGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAAAAATTCATTTTCTGACAC‑CACCCGCGCCGGGGATAAAAGGCGGAGCGGCCCTCATGCTGgtt > 2:78666/1‑146 (MQ=255) ggATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAAACATGCATTTTCTTACAC‑AACTGACGCAGGGGATAAACCGCGGAGCGCTCTTCATGCTGttttt > 1:883548/1‑143 (MQ=255) ttCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAAAAATGCTTTTTCTCACAC‑AAACTACGCAGGGGATAAACGGCGTTGCGCTCTTTTTTCTGttttt > 2:759362/1‑140 (MQ=255) tCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAAACATGCATTTTCTGACAC‑AACTGACGCAGGGGATAAACCGCGGAGCGCTCTTCATGCTGTTTGTAGATgg > 1:270623/1‑147 (MQ=255) cATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGGATAAAAAAAAAGCATTTTTTGACAC‑AACCGACGCCGGGGATAAACAGCGTTGGGCCCTTCTTGCTGttttttggtggga > 2:817549/1‑138 (MQ=255) tGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCACACAAAAAAAGCCTTTCCTGCCCC‑CAACGCCGCAAGGGGTAACAAGCGTAGGGCCCTTCATTCTGttttttgtggg > 2:899034/1‑136 (MQ=255) tGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGGATACAAAAAAATCATTTTCTGACAC‑AAAATACGCAGGGGATAAACGGCGGAGCGCTCTTCATGCTGTTTTTAGATGggagggagga > 2:549291/1‑148 (MQ=255) gTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCACAAAAATAAATCATTTTCTGAAAC‑AAACCACCCCGGGGATAAACCGCGTGGCGCCACTTACGCCGGTTTTTGATGGAAGGGAGCaactc > 2:752030/1‑144 (MQ=255) gggAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCt < 2:252141/150‑1 (MQ=255) aaGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATg < 2:80033/151‑1 (MQ=255) aGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATgg < 2:409024/151‑1 (MQ=255) gTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGgc < 2:207805/151‑1 (MQ=255) gCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACCAAACATTCCTTTTCTTACAC‑AACATACGCAGGGGGTAACCGGCGTAGCGCCCCTCATGCTGTTTGTTGATGGAAGGAAGGACCTCTGCTTATTgc > 2:630136/1‑149 (MQ=255) gCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGgcg < 1:387672/150‑1 (MQ=255) cTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAAATAATTATTTTCTTACACAAAATGCCGC‑GGGGGTAAACCGCGGAGCGCGCTTCTTTCTGTTTTTTGAAGGGAGGGAGGAcat > 2:325814/1‑135 (MQ=255) ttACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAAACATGCATTTTCTGACAC‑AACTGACGCAGGGGATAAACGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGGAGCACTTCTGCTAATAGCGcggcgg > 1:75863/1‑151 (MQ=255) cGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACt < 1:845164/151‑1 (MQ=255) ggAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCCTACAAAAAATGCAATTTCTTCCAC‑AACTGACGCAGGGGATAAACGGCGGAGGGCTCTTCATGCTGTTTTTTGATGGGAGGGAGCACTTCTGCTAATTGCGCGGCGGagt > 1:275573/1‑148 (MQ=255) ggAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTc < 2:447052/151‑1 (MQ=255) cATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAAACATGCATTTTCTTACAC‑AACTGACGCCGGGGATAAACGGCGTAGGGCTCTTCATGGTGTTTGTTGATGGAAAGTAGCACTTCTTCTAATGGCGCGTCGGGCCCaa > 1:688902/1‑149 (MQ=255) tctcTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCCTACAAAACATTCCTTTTCTGACAC‑AAACGACGCAGGGGATAAACCGCGGAGCGCTCTTCCTGCTGTTTTTTGATGGGAGGGAGCACTTCTGCTAATGGCGCGTGGGGCTCaa > 1:643007/1‑147 (MQ=255) tctTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATTCATTTTCTGGCAC‑AACTGACGCAGGGGATAAACGGCGTAGCGCTCTTCTTGCTGTTTTTAGATGGAAGGGAGGACTTCTGCTAATGGCGCGGCGGGCTCAAGCA‑Gtt > 2:481609/1‑151 (MQ=255) ttttGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑Gtt < 2:151657/148‑1 (MQ=255) tttGCAGTTGTTTCACGTTGTGGCGGGGCATA‑AAAAAATAACTTTCCTTTCAC‑CAATCACGCAGGGGATAAACGGCGGAGCGGCCTTCTTGCTTTTTTGTGGTTGAAGGGAGCACTTCTCCTCAATGCGCGTTGGaattaaaacggtttt > 1:284069/1‑136 (MQ=255) tttGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑Gttttt < 2:491772/150‑1 (MQ=255) tttGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑Gttttt < 2:486081/150‑1 (MQ=255) tttGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAAACATTCATTTTCTTACAC‑AACTGACGCAGGGGATAAACCGCGGAGCGCTCTTCATGCTGTTTGTTGATGGAAGGGAGGACTTCTGCTTATGGGGCGGCGGGCTCAAGCA‑Ggtttt > 1:383347/1‑150 (MQ=255) ttGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTc < 2:737271/150‑1 (MQ=255) tgttTCACGTTGTGGC‑GGGCCTACAAAAAAAGCATTTTCTGGCAA‑AACAGACGCCGGGGATAAACCGCGGAGCGCCCTTCATTCTGTTTTTAGATGGAAGGGAGCAATTTTGCTAATTGCGCGGCGGGGTCAAACAGGTTTTTCGagc > 2:42966/1‑146 (MQ=255) gTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAgg < 2:819249/151‑1 (MQ=255) c‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGcaacaa < 1:868410/151‑1 (MQ=255) gCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGtt > 1:643378/1‑151 (MQ=255) aaTCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAg < 1:935509/151‑1 (MQ=255) aTCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGt > 1:121308/1‑151 (MQ=255) tGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTgg > 1:841522/1‑151 (MQ=255) acac‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATAtcttct > 2:894447/1‑151 (MQ=255) aCGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTc < 1:420894/151‑1 (MQ=255) cGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTca < 2:153339/151‑1 (MQ=255) gTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTCAGCAgg > 2:820065/1‑150 (MQ=255) | TCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGC‑GGGCATACAAATCATGCATTTCCTGACAC‑AACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCA‑GTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCCATATCTTCTGCCGGAGTCAGCAGG > NC_000913/718545‑718838 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |