Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,435 | 0 | C | T | 52.5% | 27.3 / 85.7 | 59 | intergenic (‑179/‑28) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (2/26); new base T (31/0); total (33/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.54e-15 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.02e-07 |
CGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG > NC_000913/3206295‑3206585 | cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAt < 1:224823/150‑1 (MQ=255) cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACaaaaa > 1:1026134/1‑147 (MQ=255) cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCTAATTTTTCCAGACAAAAAc > 2:55434/1‑150 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACaaaaaaa > 1:418835/1‑146 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaa < 1:469698/151‑1 (MQ=255) tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTg < 1:313399/150‑1 (MQ=255) aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGtt < 1:448564/151‑1 (MQ=255) tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGtt > 2:901507/1‑149 (MQ=255) ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGAGGTggcct > 1:281969/1‑144 (MQ=255) ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGc < 1:967566/151‑1 (MQ=255) cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa < 2:781076/151‑1 (MQ=255) gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTTCCATGATGAg > 2:793077/1‑147 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTTAGACAAATAAAAAAAAATGAGGTGCCATGATGAGCGaa > 1:156636/1‑150 (MQ=255) aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCg > 2:145624/1‑147 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAAATGGGGTTGGCATGATGGGGGaaa > 1:161101/1‑149 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATTGAGTTGGCATGTTGAGGGGAAGtaa > 1:220332/1‑149 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCAGACAAAAACAAAAACTGGGGTTTTCACTGTGAGCGAAAGatataa > 1:355241/1‑150 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa < 2:27771/150‑1 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAg < 1:297548/151‑1 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCACACAAATACAAAAACTAGAGTTTGCCTGATGAGAGGAAAtaataaagaa > 1:496014/1‑144 (MQ=255) gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAAAAAATACAAAAAAAGGAGTTGCCATGATGGGGGAAAGTAATAAAGAACaaggaggg > 2:189960/1‑144 (MQ=255) gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa < 1:983308/151‑1 (MQ=255) tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGGGGTGCCATGATGAGCGAAAGGAATAAGCAAAAAGGAGGGAATaa > 1:1019438/1‑148 (MQ=255) cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTg < 2:354285/151‑1 (MQ=255) aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa < 2:360341/151‑1 (MQ=255) ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATTGAGTTTCCATGATGTGCGAAAAGAATAAACAAAAAGCAGGGAATAAGATGACAGAGAtt > 2:189206/1‑151 (MQ=255) ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGGGGTTGCATTATGAGCGAAAATAATAAACAAAAAGCAGGGAATAAATTTACaga > 1:476555/1‑147 (MQ=255) tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCCTGATTAGGGGAAGGAATAAGCAACAAGGAGGGGATAAGTTGACagag > 2:816269/1‑147 (MQ=255) tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAAAAAAAATGGAGGTGCCATGATGGGCGAAAGTAAAAAACAAAAAGCAGGGGATaaagttaaa > 2:354950/1‑137 (MQ=255) tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt < 2:913654/150‑1 (MQ=255) aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt < 1:69642/151‑1 (MQ=255) atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAATGGAGGTTGCATTATGGGCGAAAGAAAAAAACAACAAGCAGAGAAAAAGTTGAAAGAGATTg > 1:495328/1‑144 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAAAAAATAAAAAAAATTGAGGTGGCATGATGAGCGGAAAGAAAAAACAACAGGCAGGTGATAAATTTAAAGAGATTGTTGCtca > 2:299445/1‑147 (MQ=255) cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGGAAGTAAAAAACAAAAGGCAGAGAAAAAGATGACAGAGATTGTCGCTAAAttt > 1:710886/1‑150 (MQ=255) cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACttt < 2:1000338/150‑1 (MQ=255) cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACttt < 2:383935/150‑1 (MQ=255) aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATGGAGTTGGCATTATTAGCGAAAGGAATAAACAAAAGGCAATGAAAAAATTTACAGAGATTGTTGCTAACttt > 1:54227/1‑149 (MQ=255) ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg < 2:443216/151‑1 (MQ=255) ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAATGGGGTTTGCATGGTGAGGGAAAAGAATAAGAAACAAGAAGAGAATAAATTGACAGAGATTTTTGCTAAAttt > 1:362412/1‑147 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATTGGGTTGCCCTGATGAGCGGAAGTAATAAACAACAGGCCGTGAATAAGTTGACAGAGATTGTCGCTAACTTTTCCGccct > 2:783499/1‑148 (MQ=255) cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCCAGATGAGCGAAAGGAATAAACAAAAAGCAGGGAATAAATTGAAAGAGATTGTTGCTAAATTTTCcccc > 2:33966/1‑148 (MQ=255) aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGGTGTCATCATGAGCGAAAATAATAAAAAACAGGCAGGGAATAAGATGACAGAGATTGTCGCTAACTTTatcccc > 1:1007265/1‑141 (MQ=255) aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt < 1:145622/151‑1 (MQ=255) aaaaTATAAATCCACATTTTTAGTGGTAGTTTGTGGCGTTTAATTTTTCCAAACAAATACAAAAACGGGAGTTGCCATGATAAGCAAAAGTAATAAGCAACAGTCAGTTAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGAtt < 1:991285/151‑1 (MQ=255) aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCg < 2:611139/151‑1 (MQ=255) tttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGTTGTCATGATGAGCGGAAAGAATAAGCAAAAGGCAGGGGATAAGTTGACAGAGATTTTCGCTAACTTTTCCGCCATGATTTTTTCCCGAATGcc > 1:1065557/1‑149 (MQ=255) ttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGCCATGATGAGGGAAAGAAATAAACAAAAAGCAGGGAATAAGATGACAGAGATTGTCGCTAACTTTTCCGCCATGATTTTTTCCCCAATGcc > 2:678959/1‑148 (MQ=255) ttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCCTGATGAGGGAAAGAAATAAGCAACAAGCAGGGAATAAGGTGACAGAGATTGTCGCTAACTTTTCCGCCCTGATTTTTACCCGAATGCCtgat > 2:70058/1‑150 (MQ=255) tGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTGGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc < 2:791520/149‑1 (MQ=255) ggTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAGAAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTCTACCCCAAAGCCTGATGACgtg > 2:258569/1‑151 (MQ=255) ggTAGTTTGTGGCGGTAAATTTTCCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 2:101693/151‑1 (MQ=255) gTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtgg < 2:418844/151‑1 (MQ=255) gTTTGTGGCGGTGAATTTTTCCAAACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGCCAGTGAATAAGTTAACAAAGATGGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg < 1:269565/147‑1 (MQ=255) ttGTGGCGGTGAATTTTTTCCGACAAAAACAAAAAATGGAGTTGGCATGGTGAGCGAAAGTAATAAACAACAAGCAGTGAATAAAGTGGCAGAGATTTTCGCTAACTTTACCGCCATGATTTCTTCCCGAATGCCTGATGAGGtggtgg > 1:150081/1‑149 (MQ=255) ttGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGGGGTGCCATGATGAGGGGAAGTAATAAGCAAAAGGCAGTGAAAAAGTTGACAGAGAATGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg > 1:827477/1‑149 (MQ=255) ggCGGTGAATTTTTCCAGAAAAATACAAAACCTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc < 2:914311/151‑1 (MQ=255) aaTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa < 1:845251/151‑1 (MQ=255) aaTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc > 2:224403/1‑150 (MQ=255) ttttcCCAAAAAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGAAATAAGCAACAGGCAGGAAATAGGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa < 2:1026156/147‑1 (MQ=255) ccAGACAAATAAAAAAACTGGAGTTCCCATGATGCGCGAAAGTAATAACCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg < 1:1079897/151‑1 (MQ=255) ccAGAAAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa < 1:1077434/150‑1 (MQ=255) | CGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG > NC_000913/3206295‑3206585 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |