Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	46.5%	105.0 / 25.3	71	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (34/4);  new base C (0/33);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 4.64e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427                                                                                                                                                       |                                                                                                                                                       cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTAc                                                                                                                                                       >  1:891055/1‑151 (MQ=255)  aGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTAcc                                                                                                                                                      >  2:618099/1‑151 (MQ=255)    aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTAcccc                                                                                                                                                    >  2:130280/1‑151 (MQ=255)         tAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTTTTGTCAGGCAGTTCCAACCAGTTCCGCCCCCGAACCATGACGCGATGGTTGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTAccccc                                                                                                                                                   <  2:724359/147‑1 (MQ=255)           gagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCat                                                                                                                                              >  1:1010034/1‑150 (MQ=255)            agTAACACATAAAGCGTTTGCCCAGGCTGAAAATACAGCGAAATTGTTGTCAGCCAGTTCCACCCAGTCCCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTCCCCCCGCCat                                                                                                                                              <  2:105334/149‑1 (MQ=255)              tAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTc                                                                                                                                          >  2:651186/1‑151 (MQ=255)               aaCACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTCCCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCatat                                                                                                                                            <  1:773528/148‑1 (MQ=255)                   caTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAGCAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACttt                                                                                                                                     >  2:807731/1‑151 (MQ=255)                    aaaaggCGGTTCCCCAGGCTAAAAAAACAGCAAATTTGTTGTCGCCCATTCCCACCCAGCCCCCCCCCCGAACCATGACGGGATGGCCGCGGGAAAAAAAATAATCCGGGAGGCGAATT‑TTGCGGGTTTCACCCCCGCCATATTCACTTTc                                                                                                                                    <  1:792617/146‑1 (MQ=255)                    aTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACttt                                                                                                                                     >  2:744507/1‑150 (MQ=255)                             ttGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTaa                                                                                                                            >  2:409318/1‑150 (MQ=255)                             ttGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTaa                                                                                                                            >  1:968627/1‑150 (MQ=255)                                cccggtctaaaaaTACAGCAAATTTTTTTTCAGCCATTTCCACCCATCCCCCCCCCCAACCCATGGCGGGTGGGTCGCGGGAAAAAAAAAAATACGGGAAGC‑AAGATTTGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTaaa                                                                                                                           <  1:284116/138‑1 (MQ=255)                                 ccAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                       >  1:408672/1‑151 (MQ=255)                                    ggCTGAAAATACAGCAAATTTGTTGTCAGCCATTCCCAACCAGTACCCCCCCCGAACCATGACGGGATGGTCGCCGGGAAAAAAATAATACTGGAAGCGAAGA‑TTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                       <  2:645820/148‑1 (MQ=255)                                     gCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGct                                                                                                                   >  1:191166/1‑151 (MQ=255)                                       tGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctct                                                                                                                 >  2:325995/1‑151 (MQ=255)                                          aaaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                                >  1:349339/1‑149 (MQ=255)                                          aaaTACAGCAAATTTGTTGCCAGCCATTCCCACCCAGCCCCCCCCCCAAACCATGACGCGTGGGCCCCCGGAAACAAAATAATACGGGAGGCAAAGT‑TGGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                               <  1:871680/150‑1 (MQ=255)                                                    aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                    >  1:603787/1‑151 (MQ=255)                                                     aaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                    >  2:833773/1‑150 (MQ=255)                                                      aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                                  >  2:590414/1‑151 (MQ=255)                                                       ttgttgTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                                 >  1:937443/1‑151 (MQ=255)                                                         gttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                               >  1:690459/1‑151 (MQ=255)                                                            atcgcccAGTCCCATCCAGTCCCGCCCCGTAACCAGGACGGGGTGGTCGCAGGAAAAAAAATAATACGGAAGGCAAAGA‑TTGCCGGGGTGCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                                 <  1:145654/141‑1 (MQ=255)                                                               aaccAGTCCAACCCAGTCCCCCCCCCAAACCAGGACGCGATGGGCGCGGAGAACAAAATAATACTGGAAGCGAAGA‑TGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                              <  2:664069/144‑1 (MQ=255)                                                                 cacgtcccaccaaGTCCCCCCCCCAAACCATGACGCGTTGGTCGCCGGAAAAAAAATAATTCGGGAAGAAAAGT‑TGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                         <  1:351333/137‑1 (MQ=255)                                                                   attcccAACCAGTCCCCCCCCCGAACCATGACGGGATGGTCGCGGGAAAAAAAATAATACTGGAAGCGAAGT‑TTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                         <  2:122593/143‑1 (MQ=255)                                                                     ttCCAGCCAGTACCGCCCCCGAACCATGACGCGATGGCCGGCGGGAACAAAAAAATTCTGGAGGCTAATA‑TTGCGGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                     <  2:530052/149‑1 (MQ=255)                                                                     ttCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                                   >  2:113245/1‑151 (MQ=255)                                                                      tCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                                  >  1:976619/1‑151 (MQ=255)                                                                       ccAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                                 >  2:209229/1‑151 (MQ=255)                                                                         aaCCAGTACCCCCCCCAAACCATGACGGGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                                  <  1:375502/148‑1 (MQ=255)                                                                          aCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                              >  2:344397/1‑151 (MQ=255)                                                                                 ccGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                       >  1:809568/1‑151 (MQ=255)                                                                                 ccGCCCCCGAACCAGGAAGGGATGGCCGCGGGGAACAGAATAATAAGGGAGGCAAAGA‑TTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAg                                                                        <  2:566558/150‑1 (MQ=255)                                                                                    cccccGACCCATGCCGCGATGGTCGCGGGAAAAAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                     <  2:913272/150‑1 (MQ=255)                                                                                       ccaacccaggccgcgAGGGCCCCCGGGAAAAAAAAAATACGGGAGGCAAAGA‑TTGCCGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                                   <  1:852328/138‑1 (MQ=255)                                                                                       ccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                                  >  2:413627/1‑150 (MQ=255)                                                                                                  cgcgATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                      >  2:968910/1‑151 (MQ=255)                                                                                                   gggttGGTCGCCGGAAAAAGAATAATTCTGGAAGCGAAGA‑TTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                        <  2:1050002/144‑1 (MQ=255)                                                                                                   gggATGGTCGCGGGAAACAGAAAAATACTGAAAGCGAAGT‑TTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                      <  2:942952/148‑1 (MQ=255)                                                                                                     gtagcccgcggaaaaCAGAAAAATCCTGGAAGCAAAGA‑TTCCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                        <  2:904945/133‑1 (MQ=255)                                                                                                        ggTCGCGGGAAAAAAAAAAATACGGGAGGCGAAGT‑TTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                    <  1:78407/147‑1 (MQ=255)                                                                                                           cgcgGGAAACAGAAAAATACGGAAAGCAAAGA‑TTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                               <  2:242534/149‑1 (MQ=255)                                                                                                            gCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGc                                             >  1:1114710/1‑150 (MQ=255)                                                                                                              cGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                           >  2:389691/1‑150 (MQ=255)                                                                                                               gggAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                          >  1:922267/1‑150 (MQ=255)                                                                                                               gggAACAAAATAATAGTGGAGGAGAAGA‑TTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                         <  1:1106646/151‑1 (MQ=255)                                                                                                               gggAACAAAAAAATCCTGGAAGAGAAGA‑TTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCa                                                <  2:991352/144‑1 (MQ=255)                                                                                                               gggAAAAAAATAATACGGGAAGAAAAGA‑TTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                            <  1:118811/148‑1 (MQ=255)                                                                                                               ggaaaCAAAATAATAGTGAAAGCGAAGA‑TTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                          <  1:565643/147‑1 (MQ=255)                                                                                                                  aaCAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                       >  1:885864/1‑150 (MQ=255)                                                                                                                       aataatACTGGAAGCGAAGA‑TTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                                 <  1:810997/151‑1 (MQ=255)                                                                                                                         taataCTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                               >  1:550883/1‑151 (MQ=255)                                                                                                                          attaCTGGGAGGGAAGA‑TTGCGGGGATGCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                               <  1:121792/148‑1 (MQ=255)                                                                                                                          aatccggaaaGCGAAGA‑TGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                               <  2:1030800/142‑1 (MQ=255)                                                                                                                           ataCTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                              >  1:1068733/1‑150 (MQ=255)                                                                                                                           ataCTGGAAGCGAAGA‑TGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                             <  2:899352/151‑1 (MQ=255)                                                                                                                           agtgtGGAAGGGAAGT‑TGGGCGGGCTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCACCAAATAcaccaa                             <  1:869362/147‑1 (MQ=255)                                                                                                                            taCTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                             >  2:312882/1‑150 (MQ=255)                                                                                                                             acgggAAGAGAAGT‑TTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                              <  1:454992/145‑1 (MQ=255)                                                                                                                                ggacccaatta‑TTGCCGGCTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   <  1:637323/130‑1 (MQ=255)                                                                                                                                ggAAGCGAAGT‑TTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAg                           <  1:425411/148‑1 (MQ=255)                                                                                                                                gaaaGCGAAGA‑TTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                         <  2:339281/148‑1 (MQ=255)                                                                                                                                  aaGCGAAGA‑TTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                        <  2:398610/149‑1 (MQ=255)                                                                                                                                  aaGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                      >  1:702122/1‑151 (MQ=255)                                                                                                                                     cGAAGT‑TTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGATGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                     <  2:205443/149‑1 (MQ=255)                                                                                                                                                 gggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          <  2:448508/149‑1 (MQ=255)                                                                                                                                                      tACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaatact   >  2:1082798/1‑151 (MQ=255)                                                                                                                                                       aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  >  2:396291/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                       CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGA‑TTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑