| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 514,611 | 0 | C | T | 43.1% | 76.6 / 28.2 | 58 | K83K (AAG→AAA) | ybbJ | inner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX |
| Reads supporting (aligned to +/- strand): ref base C (26/7); new base T (0/25); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCC > NC_000913/514466‑514759 | cTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑g > 1:161080/1‑151 (MQ=255) gCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAGAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑g < 1:28420/149‑1 (MQ=255) gCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑Gtt < 2:185835/151‑1 (MQ=255) aCCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTc > 2:39352/1‑150 (MQ=255) cGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTcgcg < 1:830195/151‑1 (MQ=255) cGAGATCCTCACTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTcgcg > 2:922800/1‑151 (MQ=255) aTCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCAcc < 2:605005/151‑1 (MQ=255) ccTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCAccc < 1:243313/150‑1 (MQ=255) cTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACccg > 2:785395/1‑150 (MQ=255) cGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACccgccg > 2:1014133/1‑151 (MQ=255) gcgcTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGAc > 1:384441/1‑150 (MQ=255) cTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAAcc > 2:80562/1‑151 (MQ=255) accTGCCACCGCCGCGCATATGCCCGCGCCCTTTACCCGGCGAAGATCCCAACAAAAAACGTCGGCCATTCGGCTCCGCCCCGCGTTGGTTAAAATGTCTATCGCTGTGTTTTT‑GTTCGCGCCCCCGCCGCGACAACCATTTCCACCACAg < 1:962631/149‑1 (MQ=255) aaCTGTCCCCGACGCGCATATGCCCGCGCCCGTTGCCCAGGGGAGATTCCAGCAAAAAACGTCGGCAAATCAGCTGCTCCCCGCGCTGTTTTAAATGCCTATCGCTGTCTTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTccacca < 2:536419/148‑1 (MQ=255) tGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGcc > 1:701191/1‑150 (MQ=255) cACCGACGCCAATATGACCGCCCCCTTTGCCCGGCGGAGATCCCAGCAAAAACCGTCGGCCAATCAGCTGCGCCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTT‑TTTCCCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAgg < 2:187957/150‑1 (MQ=255) cggccgcgCATATGACCGCGCCCGTTGACCAGCGGAGTTCCCAGCAAAAACCGCCGCCCAATCAGCTGCTCCCCGCGCTGTTTAAAATGACTCCCGCTGTGTTTTT‑TTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAggcg < 2:354971/146‑1 (MQ=255) ccGACGCGCATATGACCGCGCCCGTTGCCCAGCGGAGATTCCAGCACAAACCGTCGGCCAATCGGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAggcg < 2:229530/150‑1 (MQ=255) ccGACGCCCATAGGCCCGCCCCCGTTGACCGGCGGAGATTCCAGCAAAAAACTCCGCCCAATCAGCTGCCGCCCGCGCTGTTTTAAATTACTACCGCGGTGTTTTT‑TTTCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAggcg < 1:637125/150‑1 (MQ=255) cGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAggcggc > 1:507552/1‑151 (MQ=255) gACGCGCATAGCCCCGCGCCCTTTGACCGGGGGAGATTCCAGCAAAAAACGCCGGCCAACCGGCTGCTGCCCGCGTTGTTTAAAATAACTATCGCTGTTTTTTT‑GTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAggcg < 2:1061188/148‑1 (MQ=255) cgcATATGCCCGCCCCCGTGCACCGGCGGAGATCCCACCACAAACCGTCGCCCAATCAGCTGCTCCCCGCGCTGTTTTAAATGACTATCGCTGTTTTTTT‑GTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc < 2:289407/150‑1 (MQ=255) cgcATAGGCCCGCCCCGGTTGACCGGCGGAGATCCCAGCAAAAACCGTCGGCCAATCAGCTGCTCCCCGCGCTGGTTTAAATGACTATCGCTGTGTTTTT‑GTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGcc < 2:832494/139‑1 (MQ=255) gcATAGGCCCGCGCCCGTTGCCCAGGGGAGTTCCCAGCAAAAACCGTCGGCCAATCGGCTGCTCCCCGCGTTGTTTAAAATGACTATCGCTGTGTTTTT‑GTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc < 1:943043/149‑1 (MQ=255) atatGCCCGCGCCCGTTGACCAGGGGAGATTCCACCACAAACCGCCGCCCAATCGGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCGGTGTTTTT‑GTTCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAggcggc < 1:953436/143‑1 (MQ=255) tatGCCCGCCCCCTTTGCCCGGCGGAGTTCCCACCACAAACCGCCGCCCAATCAGCTGCTCCCCCCGCTGTTTTAATTGCCTTCCCTTGTTTTTTT‑GTTCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagcagc < 2:766007/149‑1 (MQ=255) ccccccccttcccccAGCGAGGGTTCCAGCACAACGTCGCCGCCAACCAGCTGCTGCCCGCCGTGTTTAAAATACCTGTC‑CCGTTCTTTTGGTACGCCCCGCCGCCGCGCCAACCATTGCCACCACAGCCAGGCGGCGAGCAGCGTCAg < 1:277059/136‑1 (MQ=255) ccGCCCCCTTTGCCCAGCGGAGATCCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTc < 2:166895/147‑1 (MQ=255) ccGCCCCCGTTGACCGGCGGAGATCCCAGCACAAACCGTCGGCCAATCGGCTGCTGCCCGCGCTGGTTTAAATGCCTATCGCTGTGTTTTT‑GTTCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAgg < 2:974755/150‑1 (MQ=255) cgcCCGTTGCCCGGCGGAGATCCCAGCACAAACCGCCGGCAAACCAGCTGCGCCCCGCGCTGTTTAAAATGACTACCGCTGTGTTTTT‑TTGCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGAt < 1:697425/149‑1 (MQ=255) cccTTTGCCCAGGGGAGATTCCAGCAAAAACCGTCGCCCAATCAGCTGCTCCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTT‑GTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATg < 2:398489/148‑1 (MQ=255) cccGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGc > 2:57414/1‑150 (MQ=255) cGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCaa > 1:815618/1‑150 (MQ=255) aGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTAccc > 1:882930/1‑151 (MQ=255) gCGGAGATCCCACCACAAAACGCCGTCCAATCAGCTGCTCCCCGCGCTGGTTAAAATGATTATCGCGGTGTTTTT‑GTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACa < 1:982949/144‑1 (MQ=255) gagaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTg > 1:1012034/1‑151 (MQ=255) agaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCACGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGc > 2:185720/1‑151 (MQ=255) aaaTTCCAGCAAAAACCGCCGCCCAATCAGCTGCGCCCCGCGCTGGTTAAAATACTTACCGCTGTGTTTTT‑GTCCGCCCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGc < 1:128173/149‑1 (MQ=255) tcccAGCAAAAACCGCCGCCCAATCAGCTGCTGCCCGCGCTGTTTAAAATGCCTACCGCTGTGTTTTT‑GTTCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTAccc < 2:60637/141‑1 (MQ=255) acaAACCGTCGGCCAACCCCCGGCTGCCCCCGCTGTTTAAAATGACTACCGCTGGGTTTTT‑GTCCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCtt < 1:653569/139‑1 (MQ=255) gCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAcc > 1:123099/1‑151 (MQ=255) cccaggcAGCTGCCCCCCGCGGTGGTTAAATCTGCTGGCGTTGTGTTTTT‑CTTCGCGCGCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGc < 1:335807/142‑1 (MQ=255) cAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCAccagcc > 2:932949/1‑149 (MQ=255) ctgctgCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGac > 2:359121/1‑150 (MQ=255) tgctgcCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGacc > 2:530584/1‑150 (MQ=255) ccccCGCTGGTTAAAATGCTTACCGCTGTGTTTTT‑TTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAg < 2:482653/150‑1 (MQ=255) cccGGGTTGTTTAAAATGCCTCTCTCTGTTTTTTT‑GTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAg < 1:1050893/150‑1 (MQ=255) cgcgCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGcc > 2:58565/1‑151 (MQ=255) gcgcTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCg > 2:940769/1‑151 (MQ=255) tGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTa > 2:1089555/1‑150 (MQ=255) gTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATc > 2:260607/1‑151 (MQ=255) ttaaaaTGACTACCGTTGTGTTTTT‑GTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATc < 1:125462/147‑1 (MQ=255) ttAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCAc > 1:494595/1‑151 (MQ=255) aTGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGc > 1:441174/1‑150 (MQ=255) tgccTATCGCTGTCTTTTT‑TTCCGCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCt < 2:116671/147‑1 (MQ=255) tgtgTTTTT‑GTCCCCGCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCAcgcc < 2:315246/148‑1 (MQ=255) tgCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTcc > 1:1066622/1‑151 (MQ=255) gCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTcc > 2:875581/1‑150 (MQ=255) | CTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTT‑GTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAACCCAGCGGCACCAGCCAGACCACCAGGCCGGTAATCACCGCTGCCACGCCGCTCC > NC_000913/514466‑514759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |