Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,222,5960GA41.9% 59.4 / 47.1 62F13F (TTC→TTTymgDuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (28/8);  new base A (0/26);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.42e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAC  >  NC_000913/1222449‑1222739
                                                                                                                                                      |                                                                                                                                                 
aaCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGaaa                                                                                                                                                <  2:922613/151‑1 (MQ=255)
aaCGATGGCATTACCCATTACCGCAACAATTTCTGCAAACTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGaa                                                                                                                                                 >  2:950622/1‑150 (MQ=255)
 aCGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGaaaa                                                                                                                                               <  2:67147/151‑1 (MQ=255)
    cTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaaca                                                                                                                                             >  2:287344/1‑150 (MQ=255)
     tGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAaaacaa                                                                                                                                            >  2:287599/1‑150 (MQ=255)
         attacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑gg                                                                                                                                     >  1:202067/1‑151 (MQ=255)
           tacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGcc                                                                                                                                   >  2:622326/1‑151 (MQ=255)
             cccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGccc                                                                                                                                  <  1:855968/150‑1 (MQ=255)
             cccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCg                                                                                                                                 >  1:456755/1‑151 (MQ=255)
                attaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCa                                                                                                                              <  2:224459/151‑1 (MQ=255)
                       caacaaTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCATTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGc                                                                                                                       >  2:850984/1‑151 (MQ=255)
                        aacaaTTTCTGCAACCGGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGAAAGTAATATAGTTTTCGGACGGCAAACCGTTTTAAATATTGTAGTCCTGCCCCCAGGTCATTGGGGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCa                                                                                                                      <  1:312619/151‑1 (MQ=255)
                         acaaTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCa                                                                                                                      >  2:1019376/1‑150 (MQ=255)
                                ctccacccTGTGCGTTGTCATTTTTTGCCATTTCGTTGGTTTCTGCGAAAGAAATATAGTTTTCTGACGGCAAACCTTTTTAAATTTTGTAGTCCGGCGCCAAGGTCATGGGGGCAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAAtttt                                                                                                                <  2:527347/143‑1 (MQ=255)
                                         gtgCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTcatcatcat                                                                                                     >  2:688070/1‑151 (MQ=255)
                                         gtgCGCGGTAATTTTTTCCCTTTTCGTTGGTTTCGGGACAAGTAAAAAAGGTTTCTGACGGCAACCCTTTTTTAATATTGTAGCCCTGCGCCAAGGCCTTTGGTACAAAAACAAAAA‑‑GCCCCGCCAGTAAAGCAAATTTTTtcatca                                                                                                         <  2:1026746/147‑1 (MQ=255)
                                            cgcTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTcc                                                                                                  >  2:645475/1‑151 (MQ=255)
                                               tGTCATTTTTTGCCATTTCGTTGGTTTCTGCGAAAGTAATATAGTTTTCTGAGGGCAACCCTTTTTTAATATTGTAGTCCGGCGCCCAGGTTATTGGTGCAAAAAAAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCtt                                                                                                <  2:1005856/150‑1 (MQ=255)
                                               tGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCtt                                                                                                >  1:790070/1‑150 (MQ=255)
                                                 tcggTTTTTGCCTTTCCGTTGGCTTCTGCGCAGGTAATTTAGGTTTCGGACGGCAGCCTTTTTTTAATATTGTAGCCCGGCGCCAAGGTCATGGTTCCGAAAACAAACA‑‑TGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTa                                                                                               <  1:161605/146‑1 (MQ=255)
                                                 tCATTTTTTGCCATTTCGTTGTCTTCTGCGAAAGAAATATAGTTTTCGGACGGAAACCCTTTTTTAATATTGTGGCCCTGCCCCCGGTTCATTGGGGAAAAAAAAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTc                                                                                                   <  2:578824/145‑1 (MQ=255)
                                                 tCATTTTTTGCCATTTCGTTGGTTTCTGCGCAAGAAATATAGTTTTCTGACGACAACCCTTTTTTAATATTGTAGTCCAGCGCCCAGGTTATTGGTGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCAtt                                                                                                    <  2:250369/144‑1 (MQ=255)
                                                     tttttGCCTTTTCTTGGTTTTCTGCGAAAGAAATATAGGTTTCTGACGGCAACCCGTTTTTAATATTGTAGTCCTGCCCCCAGGTCATGGGTGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAt                                                                                              <  1:174260/146‑1 (MQ=255)
                                                     tttttGCATTTTTGTGGGTTTCTGCGAAAGTAATATGGGTTTCGGACGGAAACCGTTTTTTAATATTGTAGTCCTGCGCCCGGGTCTTGGGGGAGAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTc                                                                                           <  1:692307/149‑1 (MQ=255)
                                                       tttGCTTTTTCGTTGGTTTCTGCGAAAGTAATATAGGTTTCGGACGGCAAACCTTTTTTAATATTGTAGTCCTGCGCCCAGGTCTTGGGTGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtt                                                                                        <  1:329775/150‑1 (MQ=255)
                                                           ccATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAcc                                                                                   >  1:752822/1‑151 (MQ=255)
                                                              tttCGTTGGCTTCTGGAAAAGTAATATAGTTTTCTGACGGCAAACCGTTTTTAATATGGTAGTCCGGCGCCCAGGTAATTGGTGAAAAAAAAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAc                                                                                    <  1:980817/147‑1 (MQ=255)
                                                               ttCTTTGGCTTCTGCCAAAGTAATATAGGTTTCGGCCGGCAACCCTTTTTTAATATTGTAGTCCTCCGCCCAGGTAATGGGTGCAAAAAAAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAg                                                                                <  1:262547/150‑1 (MQ=255)
                                                                  gTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAtt                                                                            >  2:642380/1‑151 (MQ=255)
                                                                   ttgttttCTGCGAAAGTAATATAGGTTTCTGACGGCAACCTTTTTTTAATATTGTACTCCAGCGCCAGGTTTATTGGTGAAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAtt                                                                            <  2:566710/145‑1 (MQ=255)
                                                                   ttGGTTTCTGCGAAAGTAATATAGTTTTCTGACGGCAACCCTTTTTTAATATTGTAGCCCTGCGCCCAGGTCATGGGTGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                             <  1:731378/149‑1 (MQ=255)
                                                                   ttGGTTTCGGCGAAAGTAATATAGTTTTCTGACGGCAACCCTTTTTAATTTTTGGACCCCTGCGCCCAGTTCTTTGGCGAAAAAAAAAACA‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                             <  2:1017575/149‑1 (MQ=255)
                                                                        ttCTGAGAAAGAAATAGGGGTTTCTGCGGGCAACCCTTTTTTAAGTTTGTAGTCCCGCCCCCAGTTTTTTGGTGGAAAAAAAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcacc                                                                       <  2:965769/150‑1 (MQ=255)
                                                                               cAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTAc                                                               >  2:457957/1‑151 (MQ=255)
                                                                                aattAATATTGTTTTCTGACGGAAACCCTTTTTAAATTTTGTAGCCCTGCCCCAAGTTAATTGGTGGAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCg                                                                  <  1:859588/144‑1 (MQ=255)
                                                                                aaatAATATAGGTTTCTGACGGCAACCCTTTTTTAATATTGTAGTCCTGCCCCCAGGTCATTGGTGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCg                                                                  <  1:336981/144‑1 (MQ=255)
                                                                                aaGTAATATGGTTTTCTGGCGGAAACCCTTTTTAAAATTTGTAGCCCGGCGCCCAGGTTATTGGTGCAAAACAAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCAccc                                                                   <  1:268986/146‑1 (MQ=255)
                                                                                   tAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGcc                                                           >  1:10485/1‑151 (MQ=255)
                                                                                    aaTATGGGTTTCTGACGGAAACCCTTTTTAAATTTTGTAGCCCTGCGCCCAGTTCTTTGGTGCAAAAACAACCG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGcc                                                           <  1:454915/150‑1 (MQ=255)
                                                                                      tataGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAAt                                                        >  2:345102/1‑151 (MQ=255)
                                                                                        taGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATc                                                       >  1:170115/1‑150 (MQ=255)
                                                                                          gttttCTGCCGAAAAACTTTTTTTAATTTTGTACCCCTCCCCCGAGGTAATGGGTGAAAAAAAAAACA‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCaa                                                         <  1:42327/144‑1 (MQ=255)
                                                                                           gTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCAc                                                     >  2:1030546/1‑149 (MQ=255)
                                                                                             ttCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGc                                                  >  2:1048937/1‑150 (MQ=255)
                                                                                             ttCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGc                                                  >  2:84271/1‑150 (MQ=255)
                                                                                               cTGACGGCAAACCTTTTTTAAAGTTGAACCCCGGCGCCGGGGTAATGGGTGGAAAAAAAAACA‑‑GCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGc                                                  <  1:285052/148‑1 (MQ=255)
                                                                                                tGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCt                                              >  2:220710/1‑151 (MQ=255)
                                                                                                 gACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCATTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCtt                                             >  1:167055/1‑151 (MQ=255)
                                                                                                   cGGCAACCCTTTTTTATTTTGTTAGCCCGGCCCCCAGTTCTTTGGTGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTa                                           <  1:628162/151‑1 (MQ=255)
                                                                                                   agggaaacctttttttAATATTGTAGTCCTGCGCCCAGTTCTTGGGTGAAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCa                                                 <  1:853459/135‑1 (MQ=255)
                                                                                                    gacaacctgTTTTTATTTTTGTAGTCGGCCCCCAGGGTTTTTGGGGAAAAAAAAAACG‑‑GACCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCttt                                            <  2:709251/141‑1 (MQ=255)
                                                                                                     gCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAAt                                         >  2:420361/1‑151 (MQ=255)
                                                                                                        aatctttttatATTATTTTAGCCCCGCGCCAAGTTCATGGTTGAAAACAAACACTGTCCCGCCAGTAAAGCAAATTTTTTCATCACCATTCCATATTTCATTTTACCCAGAATTGCACCCCCCGTACCGCCAATCACGGCACCTTTAATc                                        <  1:342809/144‑1 (MQ=255)
                                                                                                         aCCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGcc                                     >  2:828258/1‑151 (MQ=255)
                                                                                                                        ttgttgTCCTGCCCCCGGTTTATTGGCGCGAAAACAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGa                              <  1:283072/145‑1 (MQ=255)
                                                                                                                                   cccccAGTTCTTTGGTGCAAAAACAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGa             <  2:1062286/149‑1 (MQ=255)
                                                                                                                                     cccAGGTGTTGGGGGCAAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGaa            <  1:719762/150‑1 (MQ=255)
                                                                                                                                      ccaaggtttttGGGGCAAAAAAAAACG‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCAt                        <  2:907374/129‑1 (MQ=255)
                                                                                                                                       cAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGc         >  1:667950/1‑151 (MQ=255)
                                                                                                                                           gTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGcc        >  1:720862/1‑149 (MQ=255)
                                                                                                                                           gTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGcc        >  1:153176/1‑149 (MQ=255)
                                                                                                                                                 ggTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAc  >  2:550765/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                 
AACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTTAATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACA‑‑GGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAC  >  NC_000913/1222449‑1222739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: