Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,676,106	0	C	A	46.6%	45.1 / 34.9	58	L240F (TTG→TTT)	yphD	putative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (17/14);  new base A (0/27);  total (17/41)
Fisher's exact test for biased strand distribution p-value = 1.39e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGAT  >  NC_000913/2675958‑2676252                                                                                                                                                       |                                                                                                                                                   gCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCcaacaa                                                                                                                                                  <  1:208624/151‑1 (MQ=255)   gTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACaaa                                                                                                                                                 >  2:913166/1‑150 (MQ=255)        cACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCg                                                                                                                                           >  2:843073/1‑151 (MQ=255)           cAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGTCGCCAACAAAATGCCGGTc                                                                                                                                        <  2:368287/151‑1 (MQ=255)            aGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCa                                                                                                                                       <  2:798469/151‑1 (MQ=255)             gCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCAc                                                                                                                                      <  1:82482/151‑1 (MQ=255)              cacaCCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCAcc                                                                                                                                     <  1:194161/151‑1 (MQ=255)                          tGTCCCGAACAGGGACCCGCGCCCGCCGGAAAGTGCCGTCCCGCCGACCCCGCCCGCGGCGATGACGTCAAACTCCAGCCCGTTTGC‑GGCCCCTGCGTTCCCAGAACCGAGG‑CGCGCCGCAAACAAAAGGCCGGTCACCGCCGCtaataa                                                                                                                          <  1:541425/150‑1 (MQ=255)                              ccGAACAGGGACCCGCGCCCGCCGGAAAGTGCCGTACCGCCGCCCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATcc                                                                                                                       <  1:890622/149‑1 (MQ=255)                                     gggACCCGCGCCCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGACCCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGt                                                                                                              <  1:146163/151‑1 (MQ=255)                                          ccGCGCCCGCCGAAAAGTGCCGTCCCGCCGCCCACGACCGCGGCGATGACGTCAACCTCCAGACCGTTTGC‑GGCCCCTGCGTTACCAGACCCGAGG‑CGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaaa                                                                                                           <  1:491094/149‑1 (MQ=255)                                            gcgACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGAt                                                                                                        >  2:413181/1‑150 (MQ=255)                                                  gccgGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGGAAAGATAAGAATg                                                                                                 >  1:544258/1‑151 (MQ=255)                                                   ccgGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgc                                                                                                >  2:704044/1‑151 (MQ=255)                                                   ccgAAAAGTCCCGTCCCCCCGCCCCCGCCCGCGGCGAGGACGTAAAGCCCCAGCCCGTTTGC‑GGCCCCTGCGTTCCCAAAACCGAGG‑CGCGCGGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATg                                                                                                 <  2:236575/150‑1 (MQ=255)                                                     ggAAGGGGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTCAACCTCCAGCCCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcgc                                                                                              <  1:1052106/151‑1 (MQ=255)                                                      gAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcgc                                                                                              >  2:315508/1‑150 (MQ=255)                                                      gAAAGTGCCGTACCGCCGACCACGACCGCGCCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcgc                                                                                              >  2:511818/1‑150 (MQ=255)                                                       aaggccccGTCCCGCCGACCGCGAGCGGGGCAATGCCGTAAAACCCCAGCCCTTTTGC‑GGCCCCGGCGTTCCAAAAACTGGGG‑CGCGCCACAAAAAAATTGCGGGTTACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcgc                                                                                              <  2:776857/143‑1 (MQ=255)                                                       aaaggcccgccccgccgACCACGCCCGCGGCGATGACGTCAACCCCCAGCCCTTTGGC‑GCCCCCGGCTTTCCAAGAACCGGGG‑CGCGCCGCAAAAAAAAGGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAc                                                                                            <  2:107122/140‑1 (MQ=255)                                                       aaaGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAc                                                                                            >  2:681060/1‑151 (MQ=255)                                                       aaaGGGCCGTCCCGCCGACCACGACCGCGGCGATGACGTCAAACCCCAGACCGTTTGC‑GGCACCTGCGTTCCCAGACCCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                               <  1:913600/148‑1 (MQ=255)                                                        aagcgcggccccgccgACCACGGCCGCGGCGAGGACGTCAACCTCCAGCCTGTGGGC‑GGCACCGGCTTTCCCAAAACCGAGG‑CGGGCGGCAAACAAAATGCCGGTAACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAcc                                                                                           <  2:416944/141‑1 (MQ=255)                                                         ggTGCCGTACCGCCACCCCCGACCGGGGCAATGCCGACAACCCCCAGACCGTTGGC‑GGCACCTGCGTTACCAGAACGGGGG‑GGCGCCGCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAcc                                                                                           <  2:87082/149‑1 (MQ=255)                                                          gTGCCGTACCTCCGGCCCCGCCCGGGGCGATGACTCAAAACCCCACGCGGTTTGC‑GCCCCCGGGGTTACCACAGCCGAGG‑GGCGCCGCCAACAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcg                                                                                         <  2:1049541/151‑1 (MQ=255)                                                                         ccccgcccGGGGCGATGCCGTAAAACTCCAGACGGTTGGC‑GGCCCCTGCGTT‑CCAAAACCGGGGCCGCGCCGCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTg                                                                               <  2:369890/140‑1 (MQ=255)                                                                         ccACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGcc                                                                          >  2:194179/1‑151 (MQ=255)                                                                          cACGACCGCGGCGATGACGTAAAACTCCAGACCGTTTGC‑GCCCCCGGCGTTACCAAACCCGGGG‑CGCGCCCCAAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATg                                                                            <  2:46168/148‑1 (MQ=255)                                                                               ccGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcaca                                                                     >  1:4608/1‑150 (MQ=255)                                                                               ccGCGGCAATGACGTAAAAC‑CCAGACCATTTTCTGGCCCCTGTGTTACCAAAACCAAGC‑CGCGCCGCCAAAAAAAGCCCGGTCTCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACaa                                                                    <  1:1041413/151‑1 (MQ=255)                                                                                   ggcgATGACGTCAACCTCCAGACCGTTTGC‑GGCCCCTGCGTTACCAGACCCGAGG‑CGCGCCGCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgc                                                                <  1:1043613/151‑1 (MQ=255)                                                                                   ggcgATGACGTCAAACCCCAGCCCGTTGGC‑GGCCCCGGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACt                                                                  <  2:519098/149‑1 (MQ=255)                                                                                    gcgagaccctCAAACCCCAGACGTTTTGC‑GGCACCTGCGTTCCCAGAACCGGGG‑CGCGCCCCCAAAAAAATGCCGGGCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgc                                                                <  1:48671/141‑1 (MQ=255)                                                                                        tGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATGAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGcgccgc                                                           >  2:257319/1‑151 (MQ=255)                                                                                           cGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGt                                                        >  2:174346/1‑151 (MQ=255)                                                                                               aacccccAGCCCGTTGGC‑GGCCCCTGCGTTCCCAGAACCGAGG‑CGCGCCGCCAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAg                                                      <  1:59989/144‑1 (MQ=255)                                                                                                  cTCAAGCCCGTTGGC‑GGCCCCGGCTTCCCAAGAACGGAGC‑CGGGCCCCAAAAAAAATGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCAtt                                                  <  2:239180/150‑1 (MQ=255)                                                                                                        aCCGTTGGC‑GCCCCCTGCGTTCCCAGACCGGAGG‑CGCCCCACAAAAAAAAAGCCGTTATCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGcc                                            <  1:913194/150‑1 (MQ=255)                                                                                                         ccttttGC‑GCCCCCTGCGTTCCAAGACCCGGGG‑CGCGCCGCCAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCaa                                          <  1:466905/148‑1 (MQ=255)                                                                                                         ccGTTGGG‑GGCCCCGGCGTTACCAGAACCGAGG‑CGCGCCGCCAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGcc                                            <  1:848795/149‑1 (MQ=255)                                                                                                            tttGC‑GGCCCCTGCGTTCCCAAAACCGGGG‑CGCGCCGCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACg                                        <  1:450064/150‑1 (MQ=255)                                                                                                             ttGC‑GCCCCCTGCGTTCCCAAACCGGGGG‑CGCGCCACAAAAAAAAGGCGGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACgg                                       <  1:511029/150‑1 (MQ=255)                                                                                                                    cccgtggtttACCAAACCGGGGG‑CGGGCCGCCAACAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCGCCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGc                                      <  1:671293/137‑1 (MQ=255)                                                                                                                    cACCGGCTTTCCCAGACCCGAGG‑CGCCCCCCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAAc                                <  1:845534/151‑1 (MQ=255)                                                                                                                     ccatgttactcgaaaaCCGAGG‑GCCGCAACAAACAAAGTGCTGATCACCGCCGCTAATAAACCGGAAAGGGTAAAGATAAGAATGCGCCCCCGCCGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaa                                    <  1:298162/132‑1 (MQ=255)                                                                                                                     aCCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAAcc                               >  2:883963/1‑151 (MQ=255)                                                                                                                      ccTGCGTACCCAAACCGGAGG‑CGCGCGGCAAAAAAAATGCCGTCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAAcc                               <  2:338237/150‑1 (MQ=255)                                                                                                                        tGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCg                              >  1:881681/1‑149 (MQ=255)                                                                                                                           gTTCCCAAAACGGAGG‑CGCGCCCCCAAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAgc                           <  1:911768/149‑1 (MQ=255)                                                                                                                             tcccAAAACCGAGG‑CGCGCCGCAAAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAg                            <  1:778735/144‑1 (MQ=255)                                                                                                                               ccAGACCCGGGG‑CGCGCCGCCAAAAAAAGGCCGTACACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGccc                       <  1:796635/149‑1 (MQ=255)                                                                                                                               ccAGACCCGGGG‑CGCCCCGCCAACAAAAGGCGGTTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCg                      <  1:457389/150‑1 (MQ=255)                                                                                                                                 agaccggggg‑CGCCCCGACAAAAAACGGCGGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGaa                    <  2:785003/142‑1 (MQ=255)                                                                                                                                            cgcgcCCCCAAAAAAATGCCGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGc         <  2:552627/151‑1 (MQ=255)                                                                                                                                                ccgccAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCgg       >  2:52108/1‑149 (MQ=255)                                                                                                                                                cccccAAAAAAATGCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGc         <  1:956548/144‑1 (MQ=255)                                                                                                                                                 cgccAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtg    >  2:640998/1‑151 (MQ=255)                                                                                                                                                 cgccAAAAAAAGCCCGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtg    <  1:136571/151‑1 (MQ=255)                                                                                                                                                   ccAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtgat  >  1:372660/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                   GCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGC‑GGCACCTGCGTTACCAGAACCGAGG‑CGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGAT  >  NC_000913/2675958‑2676252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑