Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,397,823	0	A	C	45.2%	41.3 / 10.1	42	V267G (GTT→GGT)	uspE	stress‑induced protein
Reads supporting (aligned to +/- strand):  ref base A (10/13);  new base C (0/19);  total (10/32)
Fisher's exact test for biased strand distribution p-value = 8.40e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTT  >  NC_000913/1397674‑1397971                                                                                                                                                       |                                                                                                                                                     aaTCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGaa                                                                                                                                                      <  1:124587/150‑1 (MQ=255)  aTCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAAcc                                                                                                                                                    <  2:279753/151‑1 (MQ=255)           ttcGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATcccc                                                                                                                                           >  1:329270/1‑151 (MQ=255)                cATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTg                                                                                                                                      <  1:373053/151‑1 (MQ=255)                           aCGGGTTTTTGATACGGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCACTCCCCTTTCCCGCC‑GTTTTGCCGAGGAATGCTGCTAAAATCCCGGTGCGTCCTACCGTGCCCAAAACCACAATCCCCGCCTGTAAGTGCt                                                                                                                              <  2:690094/148‑1 (MQ=255)                              ggTGTCTAATACTGCTCAGGTTTAATGACCAGCAGGTCGAAGCGAAGATGATCAATCCCCTGTTCCGCC‑GTTTTGCCGAGGAAGGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAAACCCAAAACCCCCGCCTGTAAGTGCTCCGcc                                                                                                                         <  1:203090/150‑1 (MQ=255)                                      atatTGGCCAGTTTTAATGCCCAGCGGGCGGCAGCGAAGACGATCAATCCCCTGTTCCCCG‑CTTTTCCAGAGGAATGTTGCGAAAATCCCGTTGCGTCCCACCTCCCCCACAACCACACCCCCCGCCTGTAAGTGCTCCGCCAAATCa                                                                                                                   <  2:7091/148‑1 (MQ=255)                                            gTCAGTTTAAATGCCCAGCAGGTCGCAGCGAAGATGATCAATCCCCTTTCCCGCC‑GTGTTGCCGAGAAATGCTGCTAAAATCCCGGGGCGCCCTACCGGGCCAAAAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGaa                                                                                                               <  2:457422/146‑1 (MQ=255)                                               aGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACctct                                                                                                       >  2:234319/1‑151 (MQ=255)                                               aGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACctct                                                                                                       >  1:144918/1‑151 (MQ=255)                                                  tttAAGGCCCACCGGGCCGAAGCGAAGATGATCAATCCCCTGTTCCGCG‑GTGTCGCCGAGGAATGCTGCTAAAATCCCGGTGCCCCCTACCGTGCCAAGACCCACATTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtctt                                                                                                      <  2:502737/149‑1 (MQ=255)                                                  ttaaaTGCCAAGCAGGTCGCAGCGAAGATGATCACCCCCCTGTTCCGCC‑GTGTTGCGGAGGAATCCTGCTAAAATCCCGGTGCGTCCTACCGTGCCCAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtcttc                                                                                                     <  2:338539/147‑1 (MQ=255)                                                   taaaTGCCCAGCAGGTCGCAGCGAAGATGATCAACCCCCTGTCCCGCC‑GTGTGGCCGAGAAAGGCGGCGAAAATGCCGGGGCTCCCTACCGTCCCAAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtcttcg                                                                                                    <  2:464840/148‑2 (MQ=255)                                                     aaTGACCAGCAGGGCCCAGCAAAGAGGATCAATCCCCTGTCCCGCC‑GTTTTGCGGAGGAATGCTGCTGAAATCCCGGTGCGTCCTCCCGTGCCCAGACCCAAAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCtcttct                                                                                                    <  1:126297/148‑1 (MQ=255)                                                              cggggcGCAGGAAAGATGATCAATCCCCTGTCCCGCC‑GTGTTGCGGAGGAATGCTGCTAAAATCCCGGGGCGCCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGAcc                                                                                            <  2:231449/142‑1 (MQ=255)                                                                ggTCGCGGCGAAGATGACAAACCCCCTGTCCCGCC‑GTGTTGCCGAGAAATGCTGCTAAAATCCCGGTGCGCCCTCCCGTGCCCAAACCCCCAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCtttt                                                                                        <  2:424448/149‑1 (MQ=255)                                                                ggTCGCAGCAAAGATGTCCAACCCCCTGTTCCCCC‑GTGTTCCCGAGAAATGCTGCTGAAATCCCGGTGCGTCCTACCGCCCCAAAACCCCCAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCtttt                                                                                        <  1:673221/149‑1 (MQ=255)                                                                          aggatgaaCAACCCCCTGTCCCGCC‑GTGTTGCCGAGGAATGCGGCTGAAATACCGGGGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTc                                                                            <  2:85336/149‑1 (MQ=255)                                                                            gatgatCAATCACCTGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCAt                                                                          >  1:285426/1‑151 (MQ=255)                                                                                   aatccCCTGTTCCGCC‑GTTTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGtttt                                                                     <  1:29287/149‑1 (MQ=255)                                                                                      caccttccccgcg‑gTTTTCCAGAGAAATGCTGCTGAAATCCCGGTGGTCCCTCCGGTGCCAAGACCCCAAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCAtt                                                                 <  1:481463/142‑1 (MQ=255)                                                                                          tGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATg                                                             >  2:448327/1‑150 (MQ=255)                                                                                          ctttCCGCCGGTGTTGAACAGAATTGCTGCTGAAATCCCGTTGCTCCTTACGGGGCCCTTACCCAATGTCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAAt                                                              <  2:257861/148‑1 (MQ=255)                                                                                            tcccgcc‑gTGTGGCAGAGGAATGCTGCTGAAATACCGGTGCCCCTTACCGTCCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGc                                                            <  1:273117/147‑1 (MQ=255)                                                                                              ccgcc‑gGGTTGCCGGGGAATGCTGCTAAAAATCCGGGGCCTCCCTCCGGGCCCAAAACCCAAACCCCCGCCTGTAAGTGCTCCGGCAAATCAGGAATTCCCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGaa                                                        <  2:619507/151‑1 (MQ=255)                                                                                                       ttGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGcc                                                >  1:551371/1‑151 (MQ=255)                                                                                                       ttGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGcc                                                >  1:144413/1‑151 (MQ=255)                                                                                                              ggAATGCTGCTGAAATCCCGGTGCGTCCTCCCGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAgg                                           <  1:16275/149‑1 (MQ=255)                                                                                                                aaTGCTGTTAAAATCCCGGTGCGTCCTACCGTCCCCACAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGc                                         <  2:326537/149‑1 (MQ=255)                                                                                                                 aTGCTGCTAAAATCCCGGTGCGTCCTCCCGTCCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCttt                                      <  2:433840/151‑1 (MQ=255)                                                                                                                     tgctgAAATCCCGGTGCTCCCTCCCGTCCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCa                                    <  1:424984/149‑1 (MQ=255)                                                                                                                         aaaaTCCCGTTGCTCCCTACCGTGCCCAAACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGcc                               <  1:284059/149‑1 (MQ=255)                                                                                                                          aaaTCCCGGTGCGTCCTCCCGTCCCCAGACCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAg                             <  1:468848/151‑1 (MQ=255)                                                                                                                             tACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCa                           >  1:446/1‑150 (MQ=255)                                                                                                                                  ggcggcccTCCCGTGCCAAAACCCCCAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGt                      <  2:68872/144‑1 (MQ=255)                                                                                                                                  gTGCGTCCTACCGTGCCCAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGtt                     <  1:109870/151‑1 (MQ=255)                                                                                                                                     cGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGcc                  >  2:419488/1‑151 (MQ=255)                                                                                                                                            ccGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAAt           <  2:719534/151‑1 (MQ=255)                                                                                                                                            ccGTGCCAAGAACCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGa             <  2:231519/149‑1 (MQ=255)                                                                                                                                             cGTGCCCAGCCCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACg              <  2:659086/147‑1 (MQ=255)                                                                                                                                             cGTGCCCAGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAAt           <  1:440686/150‑1 (MQ=255)                                                                                                                                                gcacAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCCGGGCTTTCATTGCCAGCAAATGTTGCCCACGAAt           >  2:403164/4‑147 (MQ=255)                                                                                                                                                    aGACCCACAACCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGc        <  2:421430/146‑1 (MQ=255)                                                                                                                                                      aaCCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGTATCGtt  >  2:691722/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                     AATCGTCTTCTTCGTCATCCAGTTCAACGGGTGTCTGATACTGGTCAGGTTTAATGACCAGCAGGTCGCAGCGAAGATGATCAATCACCTGTTCCGCC‑GTGTTGCCGAGGAATGCTGCTGAAATACCGGTGCGTCCTACCGTGCCCAGAACCACAATCCCCGCCTGTAAGTGCTCCGCCAAATCAGGAATCACCTCTTCTGGCAGACCTTTTTCTACGTGCGTCATGTTTTCATTAATGCCGAATTTCTGCCGCAGGGCTTTCATTGCCAGCAAATGTTGCCCACGAATGGCATCGTT  >  NC_000913/1397674‑1397971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑