| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,678,039 | 0 | T | G | 57.5% | 28.1 / 48.7 | 40 | N108T (AAC→ACC) | yphE | putative sugar ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base T (7/10); new base G (23/0); total (30/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-05 | |||||||||||
GCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGC > NC_000913/2677896‑2678186 | gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCACT‑CCCGAGGCAGAGGgttttc > 2:581067/1‑147 (MQ=255)gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCg < 2:156004/150‑1 (MQ=255)gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCg < 2:606577/150‑1 (MQ=255) cgcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACCGACCCAGGCAGAGGGTTTTCGCCCCtctc > 1:167018/1‑151 (MQ=255) ccGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGGCACTGGCCGAGGCAGAGGGTTTTCGCCCCTGt > 1:54989/1‑148 (MQ=255) ccGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCCCTGACCGAGGCAGAGGGTTTCCGCCACTGTc > 1:274308/1‑149 (MQ=255) aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTcca < 1:287811/151‑1 (MQ=255) aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTcca < 1:561652/151‑1 (MQ=255) aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACACAGGCCGAGGTGTTTCGCCCCCGTCAGCCCTTcc > 1:303126/1‑150 (MQ=255) ttGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACCGGCCGAGGCAGAGGGTTTCCCCCCCTTTCAGCCCCTccccc > 1:123172/1‑149 (MQ=255) tGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGGCCCTGACCCAGGCAGAGGGTTTTCTCCACCGTCACGCCTTcccccct > 1:603500/1‑148 (MQ=255) gAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAGCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCCCCGACCGAGGCAGAGGGTTTTCCCCCCTCTCAGCCCTTcccccc > 2:573958/1‑147 (MQ=255) aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCCCCGACCGAGGCAGAGGTTTTCCGCCCCTCTTCGCCCCTccaccccac > 2:298787/1‑146 (MQ=255) aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGATCAAAGGCGCAGTTTTCCCCCCCACTCAGCCCTTTCGCCAggc > 2:145273/1‑148 (MQ=255) aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCCCCGTCCCAGGCGGAGGGTTTCCTCCCCTTTAAGCCCCTCCCCCCCACTTTACTcc > 1:489678/1‑145 (MQ=255) cTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGGCCCTGACCCAGGCCGAGGGTTTTCCCCCCTTTCAGCCCTTCCCCCCCACTTTACTCC‑TGCTaaaac > 1:124944/1‑148 (MQ=255) tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGGCCCTGACCGGGGGAGAGGGTTTCCCCCCCTGTCAGCCCCTCCCCCCGACCTAAACCC‑TCATaaaacac > 1:686632/1‑147 (MQ=255) tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGGTTTTCGCCACTGTCCGCCCCTCCACCCGACTTAACTCC‑CCATAAAcc > 1:342531/1‑149 (MQ=255) aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGGCCCCGCCCCCGGCAGCGGGTTTTCTCCCCTCTCAACCCCTCCTCCACCCCTAACTCC‑CGACTAAcccagccgcc > 2:362185/1‑142 (MQ=255) cGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGGCACTGACCGAGGCAGAGGGTTTTCGCCCCTGTCAGCCCTTCCCCCCGGCTTTAATCC‑CTTTAAAACCCGCGcccccc > 1:81583/1‑151 (MQ=255) cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGGCCCCGACCGAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCCCCCGACTTAAATCC‑TTATAAAACGCGCGCccccccc > 2:148979/1‑150 (MQ=255) aGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGtt < 2:582392/151‑1 (MQ=255) cgcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCa < 1:235541/151‑1 (MQ=255) cgcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACCGACCGAGGCAGAGGGTTTCCGCCCCTGTCAGCCCTTCCCCCAGACTTAACTCC‑TGATAAAACGCGCGCAccccccggt > 1:575411/1‑145 (MQ=255) gcCTGTAAGCAACGTTGGGCATCCTGCGCCGTTTGCTGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAGCTCC‑TGATAAACCGCGCGCACCCCCAGTTCAg < 2:194778/151‑1 (MQ=255) gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCCCCGACCCGGGCAGAGGGTTTTCGCCACCTTCCGCCCTTCCCCCAACCCTAACTCC‑TTATAAAACCCGCccccccccccttc > 2:404170/1‑144 (MQ=255) gTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACCGACCGAGGGAGAAGGTTTTCGCCCCTGTTAGCCCTTCCACCAAACTTATCTCCTTGTTAAACC‑CCCGCACCCCCAGTTTAGCg > 1:517082/1‑149 (MQ=255) gTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACCGACCCAGGCAGAGGGTTTTCGCCCCTGTCAGCCCCTCCACCCCACTTAAATCC‑CGATAAAACCCGCGCACCCCCCCTTCAGCgg > 2:895/1‑141 (MQ=255) cTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCCCTGACCCAGGCCGAGGGTTTTCGCCACTGTCAGCCCTTCCACCCGACTTAACTCC‑TCATAAAACCCGCGCACCCCCCCTTCAGCGGGACGGCGGGTCAGCGTAg > 1:271115/1‑149 (MQ=255) cTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACCGACCGCGGCAGAGGTTTTCCGCCCCTGGCAGCCCTTCCCCCCGCCTTAACTTC‑TGCTAAACACACCGCACCCCCAGCTTAGCGGCCCGGCgcg > 2:368981/1‑138 (MQ=255) cgcCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCCCCTACCGCGGCAGAGGGTTTTCGCCCCCGTCAGCCCTTCCCCCCGACTTAAATCC‑TGATAAAACCCGCGCACCCCCACTTCAACGGCACGGCGGGGCAGCGGAGCtt > 1:610398/1‑149 (MQ=255) gcCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGGCCGAGGCCGGGGTGTTTCGCCCCCGGCAGGCCCTCCACCAGACTTAACTCC‑CGATAAAACCCGCGCACCCCCCGTTCAGCGGCACCGCGAGGCCGCCTAGcgt > 2:227043/1‑146 (MQ=255) gcCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCCGAGGGTTTCCGCCACTGTCAGCCCTTCCCCCAGACTTAACTCC‑TGATAAAACGCGCGCACCCCCAGGTCAGCGGCACGGCGAGTCAGCGGAGcct > 1:537167/1‑146 (MQ=255) tGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGGCACTGACCGAGGGAGGGGGTTTTCGCCACCGTCAGCCCTTCCACCAGACTTTACACC‑TGATAAAACCCGCGCACCCCCAGCTCAGCGGCACGGCGCGTCAGAGTAGGTTTGTCAcc > 1:475587/1‑149 (MQ=255) caatcaTGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGt < 1:345060/151‑1 (MQ=255) aatcaTGCCGTTGCGGC‑GGGGCCCCTGACCCCGGCAGAGGGTTTTCGCCCCTGTCAGCCCTTCCCCCAGACCTAACTCC‑TGATAAAACCCGCGCACCCCCCGTTCCGCGGCACGGCGAGGCAGCGTAGCCTCGTCACCTTCCAGTcgc > 1:321983/1‑148 (MQ=255) caTGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCg < 2:274845/151‑1 (MQ=255) ccGTTGCGGC‑GGGGCCACTGACCGCGGGAGAGGGTTTTCCCCCCTGTCAGCCCCTCCCCCCCACCTAAATCC‑CGATAAACCGCCCCCCCCCCCACGTCAGCGGCACGGCGAGGCAGCGGTGCCTCGTCACCCTCCAGTCGCGGCTCGcc > 2:150011/1‑149 (MQ=255) aCCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATcc < 1:211781/151‑1 (MQ=255) gagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCg > 2:688695/1‑151 (MQ=255) agGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGc > 1:713813/1‑151 (MQ=255) | GCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGC > NC_000913/2677896‑2678186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |