| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,678,043 | 0 | C | T | 46.3% | 26.5 / 18.6 | 41 | E107K (GAA→AAA) | yphE | putative sugar ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (11/11); new base T (19/0); total (30/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCG > NC_000913/2677896‑2678191 | gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCg < 2:606577/150‑1 (MQ=255)gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCg < 2:156004/150‑1 (MQ=255) cgcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCCAGGCAGAGGGTTTTCGCCCCtctc > 1:167018/1‑151 (MQ=255) ccGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTGGCCGAGGCAGAGGGTTTTCGCCCCTGt > 1:54989/1‑148 (MQ=255) ccGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGAGGCAGAGGGTTTCCGCCACTGTc > 1:274308/1‑149 (MQ=255) aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTcca < 1:287811/151‑1 (MQ=255) aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTcca < 1:561652/151‑1 (MQ=255) aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACACAGGCCGAGGTGTTTCGCCCCCGTCAGCCCTTcc > 1:303126/1‑150 (MQ=255) ttGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGGCCGAGGCAGAGGGTTTCCCCCCCTTTCAGCCCCTccccc > 1:123172/1‑149 (MQ=255) tGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACCCAGGCAGAGGGTTTTCTCCACCGTCACGCCTTcccccct > 1:603500/1‑148 (MQ=255) gAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAGCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGCAGAGGGTTTTCCCCCCTCTCAGCCCTTcccccc > 2:573958/1‑147 (MQ=255) aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGAGGCAGAGGTTTTCCGCCCCTCTTCGCCCCTccaccccac > 2:298787/1‑146 (MQ=255) aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGATCAAAGGCGCAGTTTTCCCCCCCACTCAGCCCTTTCGCCAggc > 2:145273/1‑148 (MQ=255) aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGTCCCAGGCGGAGGGTTTCCTCCCCTTTAAGCCCCTCCCCCCCACTTTACTcc > 1:489678/1‑145 (MQ=255) cTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACCCAGGCCGAGGGTTTTCCCCCCTTTCAGCCCTTCCCCCCCACTTTACTCC‑TGCTaaaac > 1:124944/1‑148 (MQ=255) tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACCGGGGGAGAGGGTTTCCCCCCCTGTCAGCCCCTCCCCCCGACCTAAACCC‑TCATaaaacac > 1:686632/1‑147 (MQ=255) tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCACTGTCCGCCCCTCCACCCGACTTAACTCC‑CCATAAAcc > 1:342531/1‑149 (MQ=255) aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGCCCCCGGCAGCGGGTTTTCTCCCCTCTCAACCCCTCCTCCACCCCTAACTCC‑CGACTAAcccagccgcc > 2:362185/1‑142 (MQ=255) cGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTGACCGAGGCAGAGGGTTTTCGCCCCTGTCAGCCCTTCCCCCCGGCTTTAATCC‑CTTTAAAACCCGCGcccccc > 1:81583/1‑151 (MQ=255) cccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGACCGAGGCAGAGGGTTTTCGCCACCGTCAGCCCCTCCCCCCGACTTAAATCC‑TTATAAAACGCGCGCccccccc > 2:148979/1‑150 (MQ=255) aGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGtt < 2:582392/151‑1 (MQ=255) cgcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCa < 1:235541/151‑1 (MQ=255) cgcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGGCAGAGGGTTTCCGCCCCTGTCAGCCCTTCCCCCAGACTTAACTCC‑TGATAAAACGCGCGCAccccccggt > 1:575411/1‑145 (MQ=255) gcCTGTAAGCAACGTTGGGCATCCTGCGCCGTTTGCTGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAGCTCC‑TGATAAACCGCGCGCACCCCCAGTTCAg < 2:194778/151‑1 (MQ=255) gcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCCGGGCAGAGGGTTTTCGCCACCTTCCGCCCTTCCCCCAACCCTAACTCC‑TTATAAAACCCGCccccccccccttc > 2:404170/1‑144 (MQ=255) gTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGAGGGAGAAGGTTTTCGCCCCTGTTAGCCCTTCCACCAAACTTATCTCCTTGTTAAACC‑CCCGCACCCCCAGTTTAGCg > 1:517082/1‑149 (MQ=255) gTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCCAGGCAGAGGGTTTTCGCCCCTGTCAGCCCCTCCACCCCACTTAAATCC‑CGATAAAACCCGCGCACCCCCCCTTCAGCgg > 2:895/1‑141 (MQ=255) cTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCCAGGCCGAGGGTTTTCGCCACTGTCAGCCCTTCCACCCGACTTAACTCC‑TCATAAAACCCGCGCACCCCCCCTTCAGCGGGACGGCGGGTCAGCGTAg > 1:271115/1‑149 (MQ=255) cTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCGCGGCAGAGGTTTTCCGCCCCTGGCAGCCCTTCCCCCCGCCTTAACTTC‑TGCTAAACACACCGCACCCCCAGCTTAGCGGCCCGGCgcg > 2:368981/1‑138 (MQ=255) cgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCTACCGCGGCAGAGGGTTTTCGCCCCCGTCAGCCCTTCCCCCCGACTTAAATCC‑TGATAAAACCCGCGCACCCCCACTTCAACGGCACGGCGGGGCAGCGGAGCtt > 1:610398/1‑149 (MQ=255) gcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGGCCGAGGCCGGGGTGTTTCGCCCCCGGCAGGCCCTCCACCAGACTTAACTCC‑CGATAAAACCCGCGCACCCCCCGTTCAGCGGCACCGCGAGGCCGCCTAGcgt > 2:227043/1‑146 (MQ=255) gcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCCGAGGGTTTCCGCCACTGTCAGCCCTTCCCCCAGACTTAACTCC‑TGATAAAACGCGCGCACCCCCAGGTCAGCGGCACGGCGAGTCAGCGGAGcct > 1:537167/1‑146 (MQ=255) tGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTGACCGAGGGAGGGGGTTTTCGCCACCGTCAGCCCTTCCACCAGACTTTACACC‑TGATAAAACCCGCGCACCCCCAGCTCAGCGGCACGGCGCGTCAGAGTAGGTTTGTCAcc > 1:475587/1‑149 (MQ=255) caatcaTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGt < 1:345060/151‑1 (MQ=255) aatcaTGCCGTTGCGGCGGGGCCCCTGACCCCGGCAGAGGGTTTTCGCCCCTGTCAGCCCTTCCCCCAGACCTAACTCC‑TGATAAAACCCGCGCACCCCCCGTTCCGCGGCACGGCGAGGCAGCGTAGCCTCGTCACCTTCCAGTcgc > 1:321983/1‑148 (MQ=255) caTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCg < 2:274845/151‑1 (MQ=255) ccGTTGCGGCGGGGCCACTGACCGCGGGAGAGGGTTTTCCCCCCTGTCAGCCCCTCCCCCCCACCTAAATCC‑CGATAAACCGCCCCCCCCCCCACGTCAGCGGCACGGCGAGGCAGCGGTGCCTCGTCACCCTCCAGTCGCGGCTCGcc > 2:150011/1‑149 (MQ=255) aCCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATcc < 1:211781/151‑1 (MQ=255) gagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCg > 2:688695/1‑151 (MQ=255) agGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGc > 1:713813/1‑151 (MQ=255) ttCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGcc < 1:703471/150‑1 (MQ=255) ccGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCg < 2:480571/149‑1 (MQ=255) | GCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCC‑TGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGCTGCCG > NC_000913/2677896‑2678191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |