Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,380 C→A G6C (GGC→TGC)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,3800CA100.0% 162.4 / NA 42G6C (GGC→TGC) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/17);  total (25/17)

GTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGA  >  NC_000913/4128234‑4128525
                                                                                                                                                  |                                                                                                                                                 
gTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTc                                                                                                                                               >  1:617878/1‑151 (MQ=255)
   ggTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCAt                                                                                                                                            <  2:175437/151‑1 (MQ=255)
        cGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGc                                                                                                                                       <  1:328406/151‑1 (MQ=255)
           gACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCa                                                                                                                                     <  1:7826/150‑1 (MQ=255)
                            tGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCctct                                                                                                                   >  1:636481/1‑151 (MQ=255)
                             gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCctc                                                                                                                    <  2:135285/149‑1 (MQ=255)
                                       gcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATaaa                                                                                                        <  2:170027/151‑1 (MQ=255)
                                           tCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                                                                                                     >  1:26187/1‑150 (MQ=255)
                                            cATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgag                                                                                                   <  2:502693/151‑1 (MQ=255)
                                             aTCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                                                                                                     >  2:704322/1‑148 (MQ=255)
                                               cGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                                                                                                <  2:259880/151‑1 (MQ=255)
                                               cGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                                                                                                <  2:616947/151‑1 (MQ=255)
                                                ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacca                                                                                               <  2:673995/151‑1 (MQ=255)
                                                 gTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccag                                                                                              <  1:226543/151‑1 (MQ=255)
                                                   gAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccaga                                                                                             <  1:418163/150‑1 (MQ=255)
                                                    aGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                                                                                           >  2:263499/1‑151 (MQ=255)
                                                     ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                                                                                           >  1:397257/1‑150 (MQ=255)
                                                      gATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcac                                                                                         >  1:139543/1‑151 (MQ=255)
                                                              acacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTg                                                                                  >  2:2955/1‑150 (MQ=255)
                                                                     aaGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGt                                                                          <  2:22832/151‑1 (MQ=255)
                                                                     aaGAGGAATGGAAACCGGAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGt                                                                          >  2:727828/1‑151 (MQ=255)
                                                                      agagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGt                                                                          >  2:75290/1‑150 (MQ=255)
                                                                           aaTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGAc                                                                    >  1:365413/1‑151 (MQ=255)
                                                                                  aCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAAc                                                             >  2:144833/1‑151 (MQ=255)
                                                                                          cTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCAt                                                       >  2:520902/1‑149 (MQ=255)
                                                                                           tttttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGt                                                    >  1:531559/1‑151 (MQ=255)
                                                                                           tttttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGt                                                    >  2:452438/1‑151 (MQ=255)
                                                                                             tttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGtt                                                   <  1:258584/150‑1 (MQ=255)
                                                                                             tttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGATGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGttt                                                  >  1:660065/1‑151 (MQ=255)
                                                                                               tGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTa                                                 <  2:594839/150‑1 (MQ=255)
                                                                                                gACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTAcc                                               >  2:205565/1‑151 (MQ=255)
                                                                                                  cTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGt                                             >  1:125705/1‑151 (MQ=255)
                                                                                                    tGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTAc                                           >  1:242501/1‑151 (MQ=255)
                                                                                                     gTTCACTCTTCTTGCCGTGCTCAGCGTATGGTCTGATATATACGCAGCGCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGACACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTAc                                           <  2:21218/150‑1 (MQ=255)
                                                                                                        cACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGc                                       >  2:177615/1‑151 (MQ=255)
                                                                                                        cACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGc                                       >  1:483642/1‑151 (MQ=255)
                                                                                                                   ccGTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGAc                            >  2:603087/1‑151 (MQ=255)
                                                                                                                     gTGCTCAGCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACAt                          >  2:428373/1‑151 (MQ=255)
                                                                                                                            gCGTATGGGCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTa                    >  2:8590/1‑150 (MQ=255)
                                                                                                                                   ggCTGATATATTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCt            <  1:376437/151‑1 (MQ=255)
                                                                                                                                          atatTCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGTACCTGTCGGCg     >  1:44632/1‑151 (MQ=255)
                                                                                                                                              tCGCAGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGa  <  1:576362/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTACTGACCGTAAACCCGCATAGTTTACCGTACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGA  >  NC_000913/4128234‑4128525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: