Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,931,977	T→G	V366G (GTG→GGG)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,931,977	0	T	G	100.0%	154.0 / NA	41	V366G (GTG→GGG)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/21);  total (20/21)

TCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGC  >  NC_000913/1931837‑1932121                                                                                                                                             |                                                                                                                                                 tCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCt                                                                                                                                        >  2:409765/1‑151 (MQ=255)      cAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGc                                                                                                                                   >  2:540469/1‑151 (MQ=255)       aGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCa                                                                                                                                  <  1:156196/151‑1 (MQ=255)        gCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGc                                                                                                                                   >  1:363314/1‑149 (MQ=255)         cTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGc                                                                                                                                   >  2:432284/1‑148 (MQ=255)              gCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAgtg                                                                                                                           >  1:471895/1‑151 (MQ=255)                cGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGtgtt                                                                                                                         <  2:638289/151‑1 (MQ=255)                 gTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGtgttg                                                                                                                        >  2:34113/1‑151 (MQ=255)                 gTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGtgttg                                                                                                                        <  1:538082/151‑1 (MQ=255)                            acaACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCAtt                                                                                                             <  2:558167/151‑1 (MQ=255)                                 tGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAAcg                                                                                                        >  2:335377/1‑151 (MQ=255)                                 tGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAAcg                                                                                                        >  2:275036/1‑151 (MQ=255)                                     cAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAAcgcgcg                                                                                                    <  2:277280/151‑1 (MQ=255)                                          aGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCa                                                                                               <  1:42049/151‑1 (MQ=255)                                                   cGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCgg                                                                                      >  1:564457/1‑151 (MQ=255)                                                       tGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGAc                                                                                    >  2:108243/1‑149 (MQ=255)                                                                  aGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTAc                                                                        >  2:446567/1‑150 (MQ=255)                                                                  aGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACa                                                                       <  1:93744/151‑1 (MQ=255)                                                                       gCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGt                                                                   <  1:114541/150‑1 (MQ=255)                                                                               cgcAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCgg                                                          <  1:399020/151‑1 (MQ=255)                                                                                gcAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCgg                                                          <  2:570614/150‑1 (MQ=255)                                                                                 cAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCAcgcc                                                                                           <  2:316999/116‑1 (MQ=255)                                                                                 cAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCAcgcc                                                                                           >  1:317025/1‑116 (MQ=255)                                                                                  aGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCa                                                        >  2:322105/1‑150 (MQ=255)                                                                                  aGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCa                                                        >  1:73915/1‑150 (MQ=255)                                                                                   gATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaa                                                      <  1:451827/151‑1 (MQ=255)                                                                                      ttGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaa                                                    <  1:539413/150‑1 (MQ=255)                                                                                       tGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGACTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaa                                                   >  1:179447/1‑150 (MQ=255)                                                                                          aGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGc                                               <  1:209132/151‑1 (MQ=255)                                                                                                   tATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACaaaaa                                       <  1:229093/150‑1 (MQ=255)                                                                                                    aattGGGAAGGCTGAAATTGATGGCAGCAGTCGTCGGGGGGGGGCACTGGCGACTGCAGAGAGTGTGGATTACGCCATTGAACGCGCGAAGCACCCCGCCTTAATGGTAAAAGTGCAGGGTTAAGCCCGGCAAAAAAACCATATAAAAAt                                      <  2:457564/148‑1 (MQ=255)                                                                                                     tttGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTATAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGc                                    >  1:636364/1‑151 (MQ=255)                                                                                                       tGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGccc                                  >  2:533460/1‑151 (MQ=255)                                                                                                               cGGAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCg                          <  1:655641/151‑1 (MQ=255)                                                                                                                 gAAATTGATGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGAt                        <  1:568172/151‑1 (MQ=255)                                                                                                                  aaaTTGGTGGCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAAGGCCCGATCCTCGAt                        <  2:533804/150‑1 (MQ=255)                                                                                                                          ggCAGCCGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttt               <  2:356677/151‑1 (MQ=255)                                                                                                                               ccGTCGTCTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACt          >  2:436103/1‑151 (MQ=255)                                                                                                                                 gtcgtcTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACttt        >  2:619931/1‑151 (MQ=255)                                                                                                                                   cgtcTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt       >  1:87928/1‑150 (MQ=255)                                                                                                                                    gtcTGGGGGGGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc     <  2:699836/151‑1 (MQ=255)                                                                                                                                        ggggggggCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGc  <  2:15486/150‑1 (MQ=255)                                                                                                                                         ggggggGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAACTCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt       <  1:569483/144‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                 TCCTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGC  >  NC_000913/1931837‑1932121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑