Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,550	T→G	W100G (TGG→GGG)	gabP →	gamma‑aminobutyrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,550	0	T	G	100.0%	181.6 / NA	48	W100G (TGG→GGG)	gabP	gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/20);  total (28/20)

TATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACTACG  >  NC_000913/2794403‑2794697                                                                                                                                                     |                                                                                                                                                    tATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCCCGCCC‑GATACCGGGTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGGTGTACtggggg                                                                                                                                                   >  1:453182/1‑150 (MQ=255)    cTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTTCCACGCCC‑GCTACCGGGTCGGTTTTCCCCTATGCCGATAAAGCCATTTGGCGCTGGGGGGGGTATACCCTCCGCCGGGTGGACTGGGGGgttt                                                                                                                                               >  2:462337/1‑151 (MQ=255)     tGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCCCGCCC‑GAAACCGGTTCGTTTTTCCCCTATGCCGATAAAGCCCTTTGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGgttt                                                                                                                                               >  1:709984/1‑150 (MQ=255)      gTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGttt                                                                                                                                                <  2:517794/148‑1 (MQ=255)        tCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTggg                                                                                                                                            <  2:548507/150‑1 (MQ=255)            cGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GGTACCGGGTTGGTTTTCACCTATGCCGGTAAAGCCATTGGGCGCTGGGCGGGGTATACCATCGGCTGGGTGTTCTGGGGGGTTTggg                                                                                                                                            >  2:311880/1‑146 (MQ=255)                  aCTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTAt                                                                                                                                  <  1:533454/150‑1 (MQ=255)                          ttATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑CATACCGGGTCGTTTTTCCCCTATGCCGATAAAACCATTGGGCGCTGGGCGGGGTATACCATCGGGTGGGTGGACTGGGGGGTTTGGGGACTGGtttttcccccggg                                                                                                                         >  2:617227/1‑140 (MQ=255)                            aTGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAg                                                                                                                       <  1:502391/151‑1 (MQ=255)                                  aTGCGGATGTTGGCGGAAATGGCGGTTTGCACGCCCGGAAACGGGTTGGTTTT‑CACCTATGCCGAGAAAGCCATTGGTCGCTGGGGGGGGGCTACCACCGGCTGGGTGTTCTGGGGGGTTTTGGGACTGGGTTTCCCCCTGGGAGc                                                                                                                      >  2:393481/1‑146 (MQ=255)                                  aTGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACa                                                                                                                 <  1:18218/151‑1 (MQ=255)                                   tGCGGATGTTGGCGGAAATGGCGGTTGCCCCGCCC‑GATACCGGTTTGGTTTTCGCCTATGCCGATGAAGGCATTGGGCGCTGGGGGGGGTATACCAACGGTTGGTTGTTCTGGGGGGTTTGGGTACTGGTTTTCCCGCTGGAAGcccaa                                                                                                                  >  2:674437/1‑146 (MQ=255)                                     cGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAACCATTTGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTTGGTACTGGTTATCCCGCTGGAAGCCaa                                                                                                                   >  1:562344/1‑146 (MQ=255)                                     cGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GATACCGGGTCGTTTTTCACCTATGCCGATAAAACCATTTGGCGCTGGGCGGGCTATTCCATCGGCTGGGTGTACTGGGGGGTTTGGGGACTGGGTATTCCGCTGGGAGcccaaa                                                                                                                 >  2:591717/1‑144 (MQ=255)                                                    aTGGCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGc                                                                                               <  2:460467/151‑1 (MQ=255)                                                       gCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCGGATAAGGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCAcg                                                                                            <  2:319515/151‑2 (MQ=255)                                                            tGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATg                                                                                        <  1:702805/150‑1 (MQ=255)                                                              ccACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGt                                                                                     <  1:232146/151‑1 (MQ=255)                                                                        aTACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCAt                                                                            <  1:486169/151‑1 (MQ=255)                                                                         tACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCAtt                                                                           >  2:639502/1‑151 (MQ=255)                                                                           ccGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTcc                                                                         >  1:565686/1‑151 (MQ=255)                                                                              gTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCAt                                                                      >  2:323659/1‑151 (MQ=255)                                                                                 cGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCt                                                                    <  2:438336/150‑1 (MQ=255)                                                                                 cGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTg                                                                   >  1:588527/1‑151 (MQ=255)                                                                                  gTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTgg                                                                  >  2:585341/1‑151 (MQ=255)                                                                                   ttttCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGt                                                                 >  1:133016/1‑151 (MQ=255)                                                                                             aTGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTccc                                                        <  2:332656/150‑1 (MQ=255)                                                                                             aTGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTcc                                                         <  1:683259/149‑1 (MQ=255)                                                                                                ccGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGt                                                    >  2:340389/1‑151 (MQ=255)                                                                                                ccGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGt                                                    >  2:389075/1‑151 (MQ=255)                                                                                                 cGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATTCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGCTCCAGGCATTCCCATCTGGATATTTTCCCTCGTc                                                   >  2:416321/1‑151 (MQ=255)                                                                                                 cGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTc                                                   <  2:669279/151‑1 (MQ=255)                                                                                                     aaaGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCAtt                                                >  2:71821/1‑150 (MQ=255)                                                                                                      aaGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTa                                               >  2:252231/1‑150 (MQ=255)                                                                                                       aGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTAcc                                             >  2:185367/1‑151 (MQ=255)                                                                                                        gCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTAccc                                            <  1:466570/151‑1 (MQ=255)                                                                                                           aTTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCg                                         <  1:112383/151‑1 (MQ=255)                                                                                                             tGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGcc                                       >  1:711307/1‑151 (MQ=255)                                                                                                             tGGGCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGcc                                       >  2:204345/1‑151 (MQ=255)                                                                                                                    tGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTg                                >  1:155064/1‑151 (MQ=255)                                                                                                                     gggcgggcTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTgg                               <  1:644671/151‑1 (MQ=255)                                                                                                                         gggcTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTCATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGt                           <  1:345554/151‑1 (MQ=255)                                                                                                                          ggcTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGt                           >  1:726719/1‑150 (MQ=255)                                                                                                                          ggcTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGt                           >  2:250507/1‑150 (MQ=255)                                                                                                                           gcTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTa                          >  2:433793/1‑150 (MQ=255)                                                                                                                                 ccATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACttatt                   >  1:168276/1‑151 (MQ=255)                                                                                                                                        cTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAGGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGt             <  2:547628/150‑1 (MQ=255)                                                                                                                                           gTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTaa          <  2:6745/150‑1 (MQ=255)                                                                                                                                             tGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTaaaa        >  1:606610/1‑150 (MQ=255)                                                                                                                                                  tgggggTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACTACg  >  1:479336/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCC‑GATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACTACG  >  NC_000913/2794403‑2794697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑