Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,347,480	IS5 (+) +4 bp	coding (1430‑1433/1935 nt)	rnb ←	ribonuclease II

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1347480 =	0 (0.000)	38 (0.760)	33/298	0.9	100%	coding (1433/1935 nt)	rnb	ribonuclease II
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1347483	0 (0.000)	47 (0.940)	44/298	0.3	100%	coding (1430/1935 nt)	rnb	ribonuclease II
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

CGTTGCTGAAAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1347627‑1347480 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTT  >  NC_000913/2101749‑2101893                                                                                                                                                                                                                                                                                                       CGTTGCTGAAAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGG                                                                                                                                                 >  2:387348/1‑150  GTTGCTGAAAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAA                                                                                                                                               <  1:603879/151‑1  GTTGCTGAAAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAA                                                                                                                                               <  1:246046/151‑1   TTGCTGAAAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGA                                                                                                                                                >  1:426114/1‑149     GCTGAAAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGT                                                                                                                                            <  2:244997/151‑1         AAAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGA                                                                                                                                        <  2:377092/151‑1          AAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAA                                                                                                                                       <  1:491877/151‑1          AAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCACGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAA                                                                                                                                       <  2:758428/151‑1               CACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGC                                                                                                                                  <  2:417329/151‑1                 CGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCG                                                                                                                                 <  2:487152/150‑1                       GCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAA                                                                                                                            <  1:243151/149‑1                         ATGTCGCTGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATT                                                                                                                         >  2:265772/1‑150                             CGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCT                                                                                                                    >  1:438475/1‑151                                TGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGG                                                                                                                 >  1:638098/1‑151                                  CCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT                                                                                                               <  2:504908/151‑1                                     AAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAC                                                                                                            >  2:370396/1‑151                                        AAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCA                                                                                                         <  1:572179/151‑1                                                ACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTACTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTC                                                                                                 <  1:766906/151‑1                                                     GGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTC                                                                                            <  2:232501/151‑1                                                       ACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTT                                                                                          <  2:99516/151‑1                                                        CGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTC                                                                                         <  1:49995/151‑1                                                                GCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTT                                                                                    <  1:405304/148‑1                                                                   AACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCC                                                                              >  2:468197/1‑151                                                                                       GCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATG                                                          <  2:394704/151‑1                                                                                          CAACCAACTGGTTTCCTCGACAGCCGCATTCGTCACTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCT                                                       >  1:267297/1‑151                                                                                             CCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGA                                                    <  2:310552/151‑1                                                                                                     TTTCCTCGACAGCTGCATACGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAACAAGCGGGGTAATTCTTCTCGGCTGACTCGGTCCTTTCATTTCTTAATGTTTGAGCCGATTTTTTCTCCCTTAAATGCCTTGAATCAGCC                                             >  2:646701/1‑150                                                                                                        CCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATT                                         <  1:754028/151‑1                                                                                                                      TTCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTC                           >  1:507473/1‑151                                                                                                                       TCGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCG                          >  1:457223/1‑151                                                                                                                        CGTCGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCG                          <  2:623623/150‑1                                                                                                                           CGCTTCCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAT                      >  1:185449/1‑151                                                                                                                                CCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTA                   >  1:471255/1‑149                                                                                                                                CCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTA                   >  1:552128/1‑149                                                                                                                                CCAGTCATTTGCTGAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTA                   >  1:748106/1‑149                                                                                                                                             GAAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCC      >  1:260743/1‑149                                                                                                                                              AAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGT   <  1:39298/151‑1                                                                                                                                               AATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGACTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTT  >  2:433293/1‑151                                                                                                                                                                                                                                                                                                       CGTTGCTGAAAACGCACGGTCTGCATGTCGATGCCGAAGAAGTGCTCACGCTGGACGGTTTCTGCAAACTGCGTCGTGAACTGGACGCGCAACCAACTGGTTTCCTCGACAGCCGCATTCGTCGCTTCCAGTCATTTGCTGAAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1347627‑1347480 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTT  >  NC_000913/2101749‑2101893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.