Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913436,5160CA42.1% 46.5 / 17.0 38Q295K (CAA→AAA) thiLthiamine monophosphate kinase
Reads supporting (aligned to +/- strand):  ref base C (19/3);  new base A (0/16);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 8.71e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.09e-01

ATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTC  >  NC_000913/436376‑436666
                                                                                                                                            |                                                                                                                                                      
aTGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGAccgc                                                                                                                                              <  1:771071/151‑1 (MQ=255)
  gTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGAccgccg                                                                                                                                            >  1:363804/1‑151 (MQ=255)
      aaCCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGa                                                                                                                                           >  2:639321/1‑148 (MQ=255)
          ggAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGa                                                                                                                                     >  2:368953/1‑150 (MQ=255)
                         ctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTAtt                                                                                                                     >  1:305361/1‑151 (MQ=255)
                              cgcTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTg                                                                                                                 >  2:136416/1‑150 (MQ=255)
                                      gggggTGAAGATTACGATTTTTTTTTCCCGGTGCCGGAACTGACCCGGGGCGCGTGGGATGTGGCTCTCGGACCCCGGGGCGTCCCTTTTCCCTGTATCGGGAAAAGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTg                                                                                                                 <  1:464736/139‑1 (MQ=255)
                                      ggCGGTGAAGATTACGGGTTGTGTTTCACTGTGCCGGAACTGAACGGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTCCCTGTATGGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGc                                                                                                            <  1:442189/147‑1 (MQ=255)
                                         ggTAAAGATTAGGAGTTGTTTTCCCCTGTGCCGGAACTGACCCGGGGCGCGCTGGTTGTGGCTCCCGGACCCCGGGGCGTCCCTTTTCCCTGTATCGGCAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTg                                                                                                     <  1:778111/151‑1 (MQ=255)
                                          gTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGt                                                                                                    >  1:429039/1‑151 (MQ=255)
                                                aTTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACAtt                                                                                              >  1:174278/1‑151 (MQ=255)
                                                 ttaggtgttttttttCACGGTGCCGGAACTGAACCGTGGCGCGTTGGATGGGGCTCCCGGCCACTGGGGCGTACTTTTTCCTTGTGTGGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACAtt                                                                                              <  2:367652/138‑1 (MQ=255)
                                                  tACGCGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACAtt                                                                                              >  2:764435/1‑149 (MQ=255)
                                                   aCGATTTTTTTTTCCCTGTGCCGGAATTGACCCGTGGCGCGGTGGGTGGGGCCCCGGGACGCCTGGTCTTACTGTTTACTTGTATCGGGAAAAGAACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGa                                                                                           <  1:519631/151‑1 (MQ=255)
                                                        ttGTGTTCCACTGGGCCGGACCGGACCCTGGGCGCGCTGGATGGGGCCCCCGACCCCCGGGGCTTACCTTTTCCCTGTATGGGCAAAATGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTg                                                                                        <  2:720145/149‑1 (MQ=255)
                                                         tgtgTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGa                                                                                      >  2:351935/1‑150 (MQ=255)
                                                          gtgtTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGaa                                                                                     >  2:4546/1‑150 (MQ=255)
                                                             tttCACTGTGCCGGAACTGAACCGTGGCGCGTTGGATGTGGCTCTCGGACCCCTGGGCGTACCTTTTCCCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTATTACATTAGACTGGAAAgg                                                                                  <  1:41885/150‑1 (MQ=255)
                                                                cgcggtgCCGAAACGGACCGGGGGCGCGCTGGATGGGGCTCGCGGACCCGGGGGCGTACCGTTTACCTGTAGGGGGAAAAGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGatat                                                                              <  1:111780/147‑1 (MQ=255)
                                                                cACTGTGCGGGAACGGACCGGTGGCGCGCTGGATGTGGCTCTCGGACACCGGGGCGTCCCTTTTACCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGatat                                                                              <  1:66002/151‑1 (MQ=255)
                                                                    gtgCCGGAACTGACCGGGGGCGCGTGGGAGGTGCCCCGGGAACCCTGGGGCTTACTTTTTCCTTGTAGGGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGatat                                                                              <  2:359201/147‑1 (MQ=255)
                                                                       ccGGAACTGACCGGGGGCGCGCGGGATGTGGCTCCCGGACCCGGGGGCGTACCTTTTCCCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACACTAGACTGGAAAGGATATGACCAt                                                                        <  1:37230/150‑1 (MQ=255)
                                                                        cGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCGGGCCGAACCGTTTACCGGTACCGGGCAAATGACCGCGGATATGGAGGGGCTTGGTTTTTTTCGTGCCGGCGAACCTGTTCCTTTAGACGGGAAAGGAAATGACCAttt                                                                      >  2:481416/1‑151 (MQ=255)
                                                                                accggTGGCGCGCTGGATGTGGCTCTCGGACCCTTGGGGTTCCCTTTTCCCTGTACGGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACg                                                               <  1:476183/146‑1 (MQ=255)
                                                                                aaCCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGc                                                              >  1:643231/1‑151 (MQ=255)
                                                                                    ggggggggttGGTTGTGGCTCGCGAACACCGGGGCGTCCCTTTTCCTTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCAt                                                           <  1:743034/141‑1 (MQ=255)
                                                                                           gcTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGt                                                   >  1:572098/1‑151 (MQ=255)
                                                                                           gcTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGt                                                   >  1:330818/1‑151 (MQ=255)
                                                                                              ggttgggtccctcGGCCACTGGGGGGTCCCTTTTCCCTTTAGGGGAAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTcgc                                                <  1:84990/141‑1 (MQ=255)
                                                                                              gactggggcccgcGGCCACCTGGGCGCACTTTTTACTTTTTTGGGGCAAAAGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCa                                                            <  1:136451/127‑1 (MQ=255)
                                                                                                aTGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTcgcg                                               >  2:234683/1‑150 (MQ=255)
                                                                                                              cgcgggggCGTCCTGTTTTCCTGTAGCGGGAAAATGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAg                                <  2:90489/146‑1 (MQ=255)
                                                                                                                   gggCGTCCCTTTTCCCTGTGGCGGAAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATg                             <  2:777854/149‑1 (MQ=255)
                                                                                                                             tttccttgtttggggAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATcc                     <  1:153335/135‑1 (MQ=255)
                                                                                                                             tttACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTgg                 >  2:515900/1‑151 (MQ=255)
                                                                                                                              ttAACTGTAGCGGGCAAATGACCGCCGATCTCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGAATGGACAGGCTATGACCATTTTGCCACGCCATAACGATGTCGATAACAGTCGCCTGAAGCTGATTACTCCGTGGc                >  2:494582/1‑151 (MQ=255)
                                                                                                                                 ccTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATc             >  1:226812/1‑151 (MQ=255)
                                                                                                                                  cTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCt            >  2:19238/1‑151 (MQ=255)
                                                                                                                                            cAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTc  >  1:127639/1‑151 (MQ=255)
                                                                                                                                            |                                                                                                                                                      
ATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTGCTGTC  >  NC_000913/436376‑436666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑