Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,489,1740GT42.9% 51.0 / 12.6 35G315C (GGT→TGT) aldAaldehyde dehydrogenase A, NAD‑linked
Reads supporting (aligned to +/- strand):  ref base G (6/14);  new base T (15/0);  total (21/14)
Fisher's exact test for biased strand distribution p-value = 1.76e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

TTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGAAAGGATATTATT  >  NC_000913/1489028‑1489324
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ttGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCCATTTTGTa                                                                                                                                                     >  2:657124/1‑150 (MQ=255)
 tGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTgg                                                                                                                                                       <  2:548758/147‑1 (MQ=255)
 tGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTa                                                                                                                                                     <  2:787077/149‑1 (MQ=255)
  gAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCCATTTGGTAAcc                                                                                                                                                  >  1:720634/1‑151 (MQ=255)
  gAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAAcc                                                                                                                                                  >  1:15034/1‑151 (MQ=255)
                 gCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACg                                                                                                                                   <  2:87475/151‑1 (MQ=255)
                   cATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTTAATTTTGGTACCCCGCTGaa                                                                                                                                          >  2:278584/1‑142 (MQ=255)
                      cGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCCATTTTGTAACCCCGCTGAACGCAACGACAtt                                                                                                                              >  2:72661/1‑151 (MQ=255)
                              cACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTTAATTTTGTAAACCCCCCGAACGCCACGCCATTGAGAgggg                                                                                                                      >  2:426987/1‑151 (MQ=255)
                                  cgTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCg                                                                                                                  <  1:783935/151‑1 (MQ=255)
                                  cgTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAAAGCAACGACATTGCGATGGGGcc                                                                                                                   >  2:260296/1‑150 (MQ=255)
                                                   aaGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAAACCCCCTGGACGCAACGACATTGCGATGGGGGCGGTGATTAAcaccgc                                                                                                    >  2:657080/1‑148 (MQ=255)
                                                   aaGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCCCCGAACGCAACGACATTGCGATGGGGGCGTTGGTTAACGCCccgg                                                                                                  >  1:298785/1‑150 (MQ=255)
                                                          tAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTTCATTTTGTAACCCCGCTGAAAGCAACGACATTGCGATGGGGCCGCTGATTAAAGCCgcggcg                                                                                                >  2:740130/1‑145 (MQ=255)
                                                           aaCTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTTAATTTTGGAACCCCGCTGAACGCCACGACATTTGGGTGGGGGCGTTTGTTTACGCCGCGGCGCTgg                                                                                            >  2:409654/1‑148 (MQ=255)
                                                                       cGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAAATTTGTAACCCCGCTGAACGCAAAGACATTGCGAAGGGGGCGTTGATTAACACCGCGGCGCTGGGAAGGGTTGAGc                                                                                >  1:738735/1‑148 (MQ=255)
                                                                        gtgtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAACCCCCCTGAACGCAACGACAATTCGATGGGGCCGGTTGTTAACGCCCCCGCGCTGGGAAGGGTCGGGCaaa                                                                             >  2:487062/1‑150 (MQ=255)
                                                                        gtgtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCaaaa                                                                            <  2:597893/151‑1 (MQ=255)
                                                                          gtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCaaaaa                                                                           <  2:368968/150‑1 (MQ=255)
                                                                           tttATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGGTGATTAACGCCGCGGCGCTGGGAAAGGGCGAGCaaaaa                                                                           >  1:292661/1‑149 (MQ=255)
                                                                                   cAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcgcg                                                                 <  1:321956/151‑1 (MQ=255)
                                                                                       aaGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAACCCCGCTGAACGCAACGACATTGCGAAGGGGGCGTTTATTAAAGCCGCCGCGCTGGAAAGGGGCGGGCAAAAAGTGgcgcgcgc                                                                >  1:13709/1‑148 (MQ=255)
                                                                                          gCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGATTAATTTTGTGACCCCGCTGAACGCAACGACATTGCGATGGGGCGGGTGATTAAAGCCGCGGGCCCGGGAAGGGGCGAGCaaaaaaagggg                                                                     >  2:69655/1‑134 (MQ=255)
                                                                                            aTTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTTGTAACCCCCCCGAACGCAACGACATTGTGATGGGGGCGTTGATTAATGCCGCGGCGCCGGGAAGGGTCTAGCAAAAAAAGGCGGGCGCAGTag                                                           >  2:389141/1‑148 (MQ=255)
                                                                                                  gATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCCAATTGGGAACCCCCCCGAACCCAACCACCCTTGGAAGGGGCCCTTGCTTTACGCCCCCGCGCTGGGAAGGGGCGAGGCAAAAGCGGCGCGCGCCGTTGAAGAAgggg                                                  >  2:95556/1‑151 (MQ=255)
                                                                                                            tCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAAATTTGTAACCCCCCTGAACGCAACGACATTTCGATTGGGCCGTTGATTAAAGCCGCGGCGCTTGAAAAGGTCGAGCAAAAAAAAGCGCGCGCAGTAGAAAAAGGGGCGAGGGTgg                                         >  1:646603/1‑150 (MQ=255)
                                                                                                                   gCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGTGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGt                                 <  2:221349/151‑1 (MQ=255)
                                                                                                                        gTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCaa                            <  2:611706/151‑1 (MQ=255)
                                                                                                                          gAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCaaa                           <  1:661303/150‑1 (MQ=255)
                                                                                                                            aGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCg                        <  2:19019/151‑1 (MQ=255)
                                                                                                                             gCGATGCAGGCGGTTTAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCg                        <  2:396671/150‑1 (MQ=255)
                                                                                                                                     ggCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAgggg               <  1:177818/151‑1 (MQ=255)
                                                                                                                                      gCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGa              <  1:250388/151‑1 (MQ=255)
                                                                                                                                       cGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGa              >  1:636534/1‑150 (MQ=255)
                                                                                                                                        ggTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGaa             >  1:308504/1‑150 (MQ=255)
                                                                                                                                              aTTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGAAAGGatat      <  2:387312/151‑1 (MQ=255)
                                                                                                                                                  ggTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGAAAGGAtattatt  >  1:761998/1‑151 (MQ=255)
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TTGAACTGGCAGTCAAAGCCATCGTTGATTCACGCGTCATTAATAGTGGGCAAGTGTGTAACTGTGCAGAACGTGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGCAAAGCGGTAGAGGGGAAAGGATATTATT  >  NC_000913/1489028‑1489324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑