Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,678,0430CT41.0% 46.2 / 14.2 39E107K (GAA→AAA) yphEputative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (3/20);  new base T (16/0);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 2.57e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGC  >  NC_000913/2677896‑2678186
                                                                                                                                                   |                                                                                                                                               
gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTcc                                                                                                                                                <  2:726973/149‑1 (MQ=255)
     tGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGcc                                                                                                                                             >  1:601186/1‑147 (MQ=255)
      gcgcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCATGACCCAGGCAGAGGGGTTTTGCCACTg                                                                                                                                         >  1:462392/1‑150 (MQ=255)
              cTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGGCCCGACCCCGGCCGAGGGTTTTCGCCACAGTCAGccc                                                                                                                                  >  2:607628/1‑149 (MQ=255)
                tAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCCCGGCAGAGGGTTTTCGcccccttccgccc                                                                                                                                  >  2:64191/1‑136 (MQ=255)
                   cGTTGAACCAAGTTGTCAAGTACTCACGTCACCGCCCTGCCCTTGTAAGCAACGGTGGGCCGCCTGCACCCGTTGCAGGTATTCAACCATGCCGTTCCGGCGGTGCCTCTGACCGAGGCATGGGTTTTCCGCCACTGTCAGCCCTTccac                                                                                                                            <  2:570135/150‑1 (MQ=255)
                    gTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGAGCCGGGCAGAGGTTTTCCGCCACTGTCCGGCCTTccccc                                                                                                                           >  1:715736/1‑150 (MQ=255)
                     ttGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTccacc                                                                                                                           <  2:480226/149‑1 (MQ=255)
                           cAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTCACCCAGGCAGAGGGTTTTCCCCCCCGTCAGCCCCTCCCCCCGACtt                                                                                                                     >  2:319627/1‑149 (MQ=255)
                            aaGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGACACCCACCGGGGGCGAGGTGTTTCGCCACTTCTAGCCCTTcccccccacctcac                                                                                                                  >  2:469324/1‑142 (MQ=255)
                               ttgttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGAAATCAACCATGCCGTTCCGGGGGGGCCCCGGACCAAGGCGGGGGGTTTCCGCCCCAGCCTGCCCCTCTCCCAAACTTAATTcc                                                                                                               >  1:791104/1‑151 (MQ=255)
                                tgttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGATCGAGGCAGGGGCTTTTCCCCACTTTCCGCCCTTCCACCACTCTTATCTcc                                                                                                               >  1:568653/1‑150 (MQ=255)
                                 gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCt                                                                                                              <  2:778740/150‑1 (MQ=255)
                                     aGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATa                                                                                                          <  1:469386/150‑1 (MQ=255)
                                        aCTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAAcc                                                                                                      <  1:742847/151‑1 (MQ=255)
                                          tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCCGGCAGAGGGTTTTCGCCCCCGTCAGCCCCTCCCCCCAACTTAACTCCTGATaaaaccc                                                                                                    >  2:684691/1‑147 (MQ=255)
                                           aaCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACcgc                                                                                                    <  1:136302/150‑1 (MQ=255)
                                                 aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGCCCCAGGCCGAGGGTTTTCTCCACCGTCAGCCCCTCCACCCGACCTAACTCCTCATAAAACCCGCCCAcc                                                                                             >  2:45907/1‑151 (MQ=255)
                                                   cGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCCAGGCAGAGGGTTTTCGCCACTGTCTGCCCCTCCACCAGACTTAACTCCTGATAAAACCCGcccccccc                                                                                           >  1:719474/1‑151 (MQ=255)
                                                        aGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGtt                                                                                      <  1:751960/151‑1 (MQ=255)
                                                            ccTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGCCCGAGGCAGAGGTTTTTCGCCCCTCGCCGCCCCTCCACCCAACTTAACTCCTGATAAAACGCCCGcccccc                                                                                           >  2:162348/1‑142 (MQ=255)
                                                                    cAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGCCCCTGGCAGGGGGTTTTCCCCCCTTTCCGCCCCTCCCCCCGAATTAACTCCTGATAAAACACGCGGCCCCCCCCCTCAGCCGCCCgg                                                                           >  2:768188/1‑150 (MQ=255)
                                                                     aaCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGc                                                                          <  2:776972/150‑1 (MQ=255)
                                                                     aaCGTTGGGCATCCTGCGCCATTGGCAGAAAACCATTCATGCCGTGGCGGCGGGGCCACGTACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCACCAGTTCAGCGGCACgg                                                                           <  2:428321/149‑1 (MQ=255)
                                                                       cGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGCGGCAGAGGGTTTTCCCCCCTGGCAGCCCTTCCACCAGAACTAACTCCTGATAAAACCCGCGCCCCCCCCGTTCAGCGGCACcgcg                                                                         >  1:397464/1‑149 (MQ=255)
                                                                       cGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAg                                                                       <  1:728614/151‑1 (MQ=255)
                                                                          tGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGACCGAGGCCGAGGGTTTTTCCCACTCTCTGCCCCTCCCCCCCACTTTACTCCTTCTAAACCGCGCCCACCCCCAGCTCACCGGCCCGGCg                                                                         >  1:713273/1‑146 (MQ=255)
                                                                                 ccTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCTCTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGc                                                             <  1:35834/151‑1 (MQ=255)
                                                                                    gcgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCtt                                                           <  1:557556/150‑1 (MQ=255)
                                                                                        cATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCCCCACTGTCAGCCCCTCCACCAGACTTAACTCCCGATAAACCCCGCGCACCCCCACTTCAGCGGCCCAGCGGGGCCGCGTAg                                                              >  2:666661/1‑143 (MQ=255)
                                                                                           ttGCAGGAAATCAATCATGCCGTTGCGGCGGCGCCACTGAACGGGGGACAGGGTTTCCGCCACTGTCCGCCCTTCCACCAGACTTAACTCCTTATAAAACGCGCGCACCCCCCGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGttccc                                                    >  1:736693/1‑146 (MQ=255)
                                                                                              cAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTAACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTcc                                                <  1:686736/151‑1 (MQ=255)
                                                                                               aGGTAATCAATCAGGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCa                                               <  2:278337/151‑1 (MQ=255)
                                                                                               aGGTAATCAACCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCa                                               <  1:248841/151‑1 (MQ=255)
                                                                                                       aatcaTGCCGTTGCGGCGGGGCCACTTACCCAGGCCGCGGGTTTTCTCCCCCGGCAGCCCTTCCACCCCACCTAACTCCTGATAAAACACGCCCCCCCCCCCCTCCGCGGCACGGCGAGGGCGCGGAGCCTCTGCACCTTCCAATc                                            >  2:566734/1‑146 (MQ=255)
                                                                                                        atcaTGCCGATGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGtct                                      <  1:498903/151‑1 (MQ=255)
                                                                                                           aTGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGc                                   <  1:784061/151‑1 (MQ=255)
                                                                                                                     ggcggGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGat                         <  2:444158/151‑1 (MQ=255)
                                                                                                                       cggGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGatat                       <  1:142440/151‑1 (MQ=255)
                                                                                                                                            agGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGc  <  2:550100/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTCGC  >  NC_000913/2677896‑2678186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑