Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,369,969	T→A	T128S (ACC→TCC)	nanK ←	N‑acetylmannosamine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,369,969	0	T	A	100.0%	190.2 / NA	49	T128S (ACC→TCC)	nanK	N‑acetylmannosamine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (17/32);  total (17/32)

TTCCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACC  >  NC_000913/3369826‑3370110                                                                                                                                                |                                                                                                                                              ttCCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATaa                                                                                                                                          >  1:334998/1‑149 (MQ=255) ttCCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATaa                                                                                                                                          >  1:62943/1‑149 (MQ=255)   ccACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGAc                                                                                                                                      >  1:241428/1‑151 (MQ=255)      cGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCa                                                                                                                                    <  2:128998/150‑1 (MQ=255)        cAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCatat                                                                                                                                 <  1:90961/151‑1 (MQ=255)        cAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCata                                                                                                                                  <  2:274417/150‑1 (MQ=255)           ccTGTGGGCCCACAGCCGCTGCCTGGGCCGTGTGGATTGGCAAGCGTATGCCCGCTATGCCCCGCCAGACCGCCAGGGCAGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATACAGACCATAGCg                                                                                                                               <  2:646244/150‑1 (MQ=255)             tgtgCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGtt                                                                                                                            >  1:756839/1‑151 (MQ=255)              gtgCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATCCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGtt                                                                                                                            <  2:203478/150‑1 (MQ=255)                    ccACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCg                                                                                                                      <  1:45417/150‑1 (MQ=255)                       caGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCa                                                                                                                   <  2:661042/150‑1 (MQ=255)                       caGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCAt                                                                                                                  >  2:534561/1‑151 (MQ=255)                           cGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCCCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAg                                                                                                              <  2:404319/151‑1 (MQ=255)                            gCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAgc                                                                                                             <  1:205284/151‑1 (MQ=255)                             cAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAgc                                                                                                             >  2:581714/1‑150 (MQ=255)                              aGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAgcgc                                                                                                           >  2:295677/1‑151 (MQ=255)                                    ggCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGaa                                                                                                      >  1:397876/1‑150 (MQ=255)                                        gtgtGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTcc                                                                                                 >  1:683625/1‑151 (MQ=255)                                                cGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCAtg                                                                                          <  2:341399/150‑1 (MQ=255)                                                cGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCAtg                                                                                          <  1:412282/150‑1 (MQ=255)                                                    aaGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCg                                                                                     >  2:712428/1‑151 (MQ=255)                                                      gctgaTGCCCGTGATGCCCGGCGAGACCGCCAGGGCCGGTAAGAAGTTTGCAGCGCCTCGCTACACCGCCGACAAGGTCGGTGGAAACGGAGAGAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAGCTCCGCCCAtgctgc                                                                                      <  2:624629/144‑1 (MQ=255)                                                      gCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGc                                                                                   >  2:599417/1‑151 (MQ=255)                                                          aTGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTgc                                                                               <  2:125411/151‑1 (MQ=255)                                                              ccGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGcgt                                                                           <  2:201811/151‑1 (MQ=255)                                                                   atGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGtt                                                                       <  2:184617/150‑1 (MQ=255)                                                                   atGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGtt                                                                       >  1:404607/1‑150 (MQ=255)                                                                      ccccGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATg                                                                   <  1:534626/151‑1 (MQ=255)                                                                           ccAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCa                                                                <  2:424259/149‑1 (MQ=255)                                                                           ccAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAAt                                                              <  1:431966/151‑1 (MQ=255)                                                                           ccAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAAt                                                              <  1:621300/151‑1 (MQ=255)                                                                             aGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAACCTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATgg                                                            <  2:551344/151‑1 (MQ=255)                                                                             aGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATgg                                                            <  2:404550/151‑1 (MQ=255)                                                                              gACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGt                                                           <  1:281576/151‑1 (MQ=255)                                                                                   ccAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGc                                                       >  1:406485/1‑150 (MQ=255)                                                                                   ccAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCGGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCa                                                      <  2:500399/151‑1 (MQ=255)                                                                                    cAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCaa                                                     <  1:759096/151‑1 (MQ=255)                                                                                           ggTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTa                                              <  2:401794/151‑1 (MQ=255)                                                                                                gCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttg                                         >  2:519154/1‑151 (MQ=255)                                                                                                    tttGCAGCCTCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCAGGCTGCGTCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAgttgt                                        <  2:559622/148‑1 (MQ=255)                                                                                                      tGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTcc                                     >  2:460838/1‑149 (MQ=255)                                                                                                        cAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCg                                 <  1:462060/151‑1 (MQ=255)                                                                                                         aGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGt                                >  1:215225/1‑151 (MQ=255)                                                                                                              cTCACTACACCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTg                            <  2:180995/150‑1 (MQ=255)                                                                                                                    acacCGCCGCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAAc                      <  1:401144/150‑1 (MQ=255)                                                                                                                       ccgccgCCAACGCCGGTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACgg                    <  2:453950/149‑1 (MQ=255)                                                                                                                                     ggTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATcca    <  2:756740/151‑1 (MQ=255)                                                                                                                                      gTGGAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATccac   <  1:309885/151‑1 (MQ=255)                                                                                                                                        ggAAACGGAGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATccacc  >  2:586260/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                              TTCCACGCAACCTGTGCGTCCACAGCCGCAGACTGGGCCGTGTGGATCGGCAAGCGTATGCCCGATATGCCCCGCCAGACCGCCAGGGCCGGTAAGCAGTTTGCAGCCGCTCACTACACCGCCGCCAACGCCGGTGGAAACGGTGATAAAGACCATATCGGTTATATCGCCATCCAGCGCCTGAAACTCCGCCCATGCTGCGGCCTGCGCGTCGTTAATGGCAATGGTCGGCAAATTGGTAAGTTGTTCCAGCGTTTTGACTAACGGAAAGTGTAGCAATCCACC  >  NC_000913/3369826‑3370110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑