Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,583	C→T	H447Y (CAC→TAC)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,583	0	C	T	100.0%	199.8 / NA	55	H447Y (CAC→TAC)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/30);  total (25/30)

GTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCA  >  NC_000913/4182435‑4182724                                                                                                                                                     |                                                                                                                                              gTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGactac                                                                                                                                             >  1:118225/1‑151 (MQ=255)  tCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGactacc                                                                                                                                            >  1:249863/1‑151 (MQ=255)     gCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCg                                                                                                                                         <  1:661677/151‑1 (MQ=255)     gCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCg                                                                                                                                         <  1:641912/151‑1 (MQ=255)         ttGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCa                                                                                                                                      >  1:246988/1‑150 (MQ=255)           ggTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAAc                                                                                                                                    <  1:425247/150‑1 (MQ=255)              cGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACcgtc                                                                                                                                >  2:145780/1‑151 (MQ=255)                 aTGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACcgtcgt                                                                                                                              <  1:31198/150‑1 (MQ=255)                 aTGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTa                                                                                                                             <  1:205087/151‑1 (MQ=255)                  tGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTAt                                                                                                                            >  1:88182/1‑151 (MQ=255)                    aaGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCATCCGTCGTAt                                                                                                                            <  2:355190/149‑1 (MQ=255)                         cAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTAtccgttc                                                                                                                      <  2:524744/150‑1 (MQ=255)                         cAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACAGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTAtccggtcc                                                                                                                     <  2:598773/151‑1 (MQ=255)                          aaCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTAtccgttccg                                                                                                                    >  2:86214/1‑151 (MQ=255)                           aCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTAtccgttccgt                                                                                                                   <  1:242775/151‑1 (MQ=255)                           aCCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTAtccgttccgt                                                                                                                   >  1:315022/1‑151 (MQ=255)                                tctcTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGc                                                                                                               <  2:118190/150‑1 (MQ=255)                                     gctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATg                                                                                                         <  2:446904/151‑1 (MQ=255)                                      ctgcGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATgg                                                                                                        >  2:643348/1‑151 (MQ=255)                                        gcgcGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGc                                                                                                       <  1:486560/150‑1 (MQ=255)                                          gcgAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCgg                                                                                                     >  2:317225/1‑150 (MQ=255)                                           cgAAGAAATCGAAGGTGCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaa                                                                                                   <  2:387420/151‑1 (MQ=255)                                            gaagaaATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGaa                                                                                                   >  1:543320/1‑150 (MQ=255)                                                aaaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCa                                                                                              <  1:643420/151‑1 (MQ=255)                                                aaaTCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCa                                                                                              <  2:331076/151‑1 (MQ=255)                                                      aaGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCcg                                                                                        <  2:200562/151‑1 (MQ=255)                                                           tCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTg                                                                                   >  2:420423/1‑151 (MQ=255)                                                            ccGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGTGAAATGGCGGAAAACCAGTTCCGCGTTgg                                                                                  >  1:299791/1‑151 (MQ=255)                                                               gTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCt                                                                               >  1:598968/1‑151 (MQ=255)                                                                tATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTg                                                                              >  1:587982/1‑151 (MQ=255)                                                                 acccTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGGCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTg                                                                              <  2:105226/148‑1 (MQ=255)                                                                   ccTGAGCAAAGACGACATCATTGATGTTATGAAACAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGACATGGCGGAAAACCAGTTCCGCGTTGGCCTgg                                                                             >  2:411665/1‑149 (MQ=255)                                                                            aGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTag                                                                    >  1:592685/1‑149 (MQ=255)                                                                                gacATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTg                                                              >  1:645579/1‑151 (MQ=255)                                                                                   atcatTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCgg                                                           <  1:137506/151‑1 (MQ=255)                                                                                          aTGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGa                                                        >  1:453542/1‑147 (MQ=255)                                                                                           tGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAag                                                     >  1:412141/1‑149 (MQ=255)                                                                                               aTGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtc                                               >  2:310479/1‑151 (MQ=255)                                                                                                 gAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCgtcgg                                             <  2:11668/151‑3 (MQ=255)                                                                                                   aaacGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGCCATCCGGCGTATCCCTTCCGTTGGCGAAACGGCGGAAAACCAGTGCCGCGTTGGCCTGGTACTTGTAGAGCGTTCGGTGGAAGAGCgtctg                                             <  2:415364/149‑1 (MQ=255)                                                                                                        cTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTgg                                      >  2:516167/1‑151 (MQ=255)                                                                                                           aTCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCg                                   <  1:658400/151‑1 (MQ=255)                                                                                                           aTCGATATCCGTAACGGCAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCg                                   >  2:329718/1‑151 (MQ=255)                                                                                                             cGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGa                                  <  2:389284/150‑1 (MQ=255)                                                                                                               atatCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCt                               <  1:758769/151‑1 (MQ=255)                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTg                              <  1:231408/150‑1 (MQ=255)                                                                                                                 atCCGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTg                              <  2:286672/150‑1 (MQ=255)                                                                                                                    cGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAt                           >  1:584412/1‑150 (MQ=255)                                                                                                                    cGTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATa                          <  1:363413/151‑1 (MQ=255)                                                                                                                     gTAACGGTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATAc                         >  1:112955/1‑151 (MQ=255)                                                                                                                           gTAAAGGCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCACGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGAt                   >  2:122183/1‑151 (MQ=255)                                                                                                                                 gCGAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCcaca             <  2:283171/151‑1 (MQ=255)                                                                                                                                   gAAGTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAgg           <  2:88148/151‑1 (MQ=255)                                                                                                                                      gTCGATGATATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGat         >  1:387102/1‑150 (MQ=255)                                                                                                                                         gatgatATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGa     <  2:283264/151‑1 (MQ=255)                                                                                                                                          atgatATCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGag    <  1:411722/151‑2 (MQ=255)                                                                                                                                             atatCGACTACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCa  >  1:496794/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                              GTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGGTATCCTGAGCAAAGACGACATCATTGATGTTATGAAAAAGCTCATCGATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCA  >  NC_000913/4182435‑4182724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑