Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,794,550	T→G	W100G (TGG→GGG)	gabP →	gamma‑aminobutyrate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,794,550	0	T	G	100.0%	120.4 / NA	35	W100G (TGG→GGG)	gabP	gamma‑aminobutyrate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/19);  total (16/19)

TATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACTACG  >  NC_000913/2794403‑2794697                                                                                                                                                    |                                                                                                                                                    tATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGGTACCCGTTCGTTTTCTAACTATGCCGATAAAGCCCTTGGTCGCTGGGGGGGGTATACCATCGGCCGGGTTTACtgggggg                                                                                                                                                  >  2:59201/1‑150 (MQ=255)  aTCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACtggggg                                                                                                                                                   <  2:85647/149‑1 (MQ=255)      gTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTg                                                                                                                                              <  2:462983/150‑1 (MQ=255)       ttCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTTCCACGCCCGATACCGGGTCGTTTTTCACCCATGCCGATAAAGCCATTGGTCGCCGGGCGGGCTATACCATCGGCTGGGTGTACTGGGGGTTTTtgg                                                                                                                                            >  1:674573/1‑151 (MQ=255)         cGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTTCACCTATGCCGATAAAGCCCTTGGTCGCTGGGCGGGCTATACCATCGGCTGGGTGTTCTGGGGGGTTTggg                                                                                                                                            >  1:471050/1‑149 (MQ=255)           ccGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACt                                                                                                                                        <  2:768356/151‑1 (MQ=255)                  aCTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTa                                                                                                                                   <  1:502483/149‑1 (MQ=255)                               aTTATGCGGATGTTGGCGGAAATGGCGGCTGCCACGCCCGATACCGGGTCGTTTTTTACCCATTCCGATAAAGCCATTGGGCGCGGGGCGGGATATACCATCGGGTGGGTGTACTGGGGGGTTTGGGGAATGGGTATCCCGCCgggaacc                                                                                                                     >  2:203153/1‑145 (MQ=255)                                             ggCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCAt                                                                                                       <  2:98169/150‑1 (MQ=255)                                                  aaaTGGCGGTGGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCt                                                                                                 <  2:266174/151‑1 (MQ=255)                                                      ggCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCAc                                                                                             <  2:569563/151‑1 (MQ=255)                                                            tGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATg                                                                                        <  2:676301/150‑1 (MQ=255)                                                               cACGCCCGTTACCTGGTCGTTGTCCACCTATGCCGATAAAACCATTGGCCGCTGGGCGGGCTATACCATCGGATGGCTGAACTGGGGGTTTTGGGTACTGGGTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATggggt                                                                                    >  2:110674/1‑149 (MQ=255)                                                                    ccGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTAGGCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAgg                                                                               <  2:43296/151‑1 (MQ=255)                                                                     cGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGc                                                                              <  1:545955/151‑1 (MQ=255)                                                                      gATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGc                                                                              >  2:182235/1‑150 (MQ=255)                                                                          tcGGTTCGTTTTCCACCTATGCCGGTAAAGCCATCGCTCGCTAGGCGGCCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTAATGGGTTCCACGCATTc                                                                          >  2:583132/2‑150 (MQ=255)                                                                                 ggtttCCACCTATTCCGATAAATCCATTGGTCGCTGGGGGTGCTATACCATCGGCTGGTTGAACTAGGGGTTTTGGGGACCGGTTATCCCGCTCGAAGCAAACATTGCCGCCATGATACGGCACTCATGGGTTCCCGGCa                                                                             >  2:630740/3‑140 (MQ=255)                                                                                 gTTTTCCACCAATGCCGATACAGACATTGGTCCCTGGGCGGGCTATACCATCGGCTGGCTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGTTCCTTCATTCATGGGCTCCAGGCATTCCCATc                                                                     >  2:771817/1‑148 (MQ=255)                                                                                   tttCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGtt                                                                >  2:163244/1‑151 (MQ=255)                                                                                   tttCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGt                                                                 <  1:61075/150‑1 (MQ=255)                                                                                       cACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTAttt                                                            <  2:547299/151‑1 (MQ=255)                                                                                         ccTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTc                                                          <  1:128159/151‑1 (MQ=255)                                                                                            aTGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTccc                                                        >  2:732386/1‑150 (MQ=255)                                                                                                    aaaGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCAtt                                                >  1:16145/1‑150 (MQ=255)                                                                                                      aGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCCCGTCATTAcc                                             >  2:700163/1‑151 (MQ=255)                                                                                                             ggTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCt                                      <  1:343036/151‑1 (MQ=255)                                                                                                                 gCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAAc                                  >  2:135461/1‑151 (MQ=255)                                                                                                                   tGGGCGGGCTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTg                                >  1:407863/1‑151 (MQ=255)                                                                                                                     ggcgggcTATACCATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGc                              <  2:386283/151‑1 (MQ=255)                                                                                                                      gcgggcTATCCCCTCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCCCTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACt                                 <  2:216507/147‑1 (MQ=255)                                                                                                                                ccATCGGCTGGTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACtta                     <  1:365957/149‑1 (MQ=255)                                                                                                                                      gCTGGTTGTACTGGGGGTTTTGGGTAATGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGc               <  2:550904/149‑1 (MQ=255)                                                                                                                                         ggTTGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGtt            <  1:135496/149‑1 (MQ=255)                                                                                                                                           ttGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTaaa         >  1:737935/1‑150 (MQ=255)                                                                                                                                            tGTACTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTaaaa        <  2:451419/150‑1 (MQ=255)                                                                                                                                               aCTGGGGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACt     >  2:761756/1‑150 (MQ=255)                                                                                                                                                 tgggggTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACTACg  >  2:465753/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TATCTGTTCGCCGGGCTACTGGTGGTTATGATTATGCGGATGTTGGCGGAAATGGCGGTTGCCACGCCCGATACCGGTTCGTTTTCCACCTATGCCGATAAAGCCATTGGTCGCTGGGCGGGCTATACCATCGGCTGGTTGTACTGGTGGTTTTGGGTACTGGTTATCCCGCTGGAAGCCAACATCGCCGCCATGATCCTGCACTCATGGGTTCCAGGCATTCCCATCTGGTTATTTTCCCTCGTCATTACCCTCGCCTTAACTGGCAGTAACTTATTAAGCGTTAAAAACTACG  >  NC_000913/2794403‑2794697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑