Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,621,118 | 0 | A | C | 44.1% | 96.7 / 14.4 | 60 | V7G (GTG→GGG) | eamA | cysteine and O‑acetyl‑L‑serine efflux system |
Reads supporting (aligned to +/- strand): ref base A (25/8); new base C (0/26); total (25/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.04e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTT > NC_000913/1620970‑1621267 | tACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACAcc > 1:75891/1‑151 (MQ=255) aCTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACAcc < 1:235139/150‑1 (MQ=255) gTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCttt < 1:532750/151‑1 (MQ=255) gTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCttt > 2:2842/1‑151 (MQ=255) tCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCtttt > 1:365938/1‑151 (MQ=255) tCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCtttt > 1:256711/1‑151 (MQ=255) gCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTcgcg > 2:219525/1‑151 (MQ=255) cAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACAt < 1:758693/151‑1 (MQ=255) cAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACAt > 1:520987/1‑151 (MQ=255) aaaaGCGACCAGCAAAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCAGACGACCCCGCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAAt < 2:199181/151‑1 (MQ=255) aggCGACCAGCATAAAGCGCAACCCGGCCACCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTACCCCCCATCCGCCCCCTCCCAGTAGCGCCACCCCCCCATCTTTCCCCGACATTCTACCGCCTCTGAAttt < 2:440218/149‑1 (MQ=255) aGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCATCTGAAttt > 1:812529/1‑150 (MQ=255) aCCAGAATAAAGCGCAAACCGGCCAGCATCAGTGGTGGCATGTTATGAAGCCCCACTTTGATGACCAAAAAATTTACCCCCCATACGCCCCCTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCAt < 2:164988/149‑1 (MQ=255) cAGCAAAAAGCGCAACCCGGCCAGCATCAGCGGTGGCATTTTATGAAGCCCCACTTTGATGACCACAAAATTTACCCCCCATCCGCCCCCTCCCATTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCt < 2:7285/149‑1 (MQ=255) aGCATAAAGCGCAACCCGGCCAGCATCAGCGGTGGCATTTTATGAAGCCCCACTTGGATGACCACAAAATTTACCCCCCATACGACCCCTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCtttt < 1:573895/151‑1 (MQ=255) aGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCtttt > 1:226674/1‑151 (MQ=255) aTAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTGGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGt > 2:692902/1‑150 (MQ=255) aTAAAGAGAAAACCGCCCACCACCCGCGGTGGTATTTTATGAACCCCAATTTTAATGACCAAAAAATTTCGCCCCAATACGCCCCCTCCCAATACCCCCACCCCCCCACCTTTCCCCTCCTTTCTACCGCCTCTGAATTTCATCtttt < 2:836860/148‑1 (MQ=255) aaGCGCAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGCCCCCTACCAGTAGCGCCACCCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGc < 1:444408/151‑1 (MQ=255) aaGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGc > 1:147803/1‑151 (MQ=255) aGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAg > 2:424651/1‑149 (MQ=255) gcccAACCCGCCCAGCATAAGGGGTGGCATGTTATAAACCCCCCTTTTGTTGACCACAAAATTTACCCCCCAAACGACCTCTCCCATTAGCTCCAACACCCCATCTTTTCCCAACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGc < 2:878581/146‑1 (MQ=255) cccAACCCGCCCACCACCAGCGGGGGCATGTAATGAGCCCCCATTTTGATGACCAAAAAATTTAGCCCCCAAACGCCCTCTCCCGGTAGCTCCAACCCCCCATCTTTTCCCGACTTGCTACCGCCTCTGAATTTCATCTTTTGTAAGc < 1:403784/146‑1 (MQ=255) aacccGGCCAGCATCAGCGGTGGCATTTAATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATCCGCCCCCTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCaa < 2:620705/148‑1 (MQ=255) aaaCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCCCTTTGATGACCACAAATTTTAGCCCCCATACGCCCACTCCCAGTAGCGCCAACCCCCAATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAAt < 1:40592/148‑1 (MQ=255) aaCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAAc > 2:271640/1‑151 (MQ=255) aaCCGGCCAGAATCAGCGGTGGCATGTTATGAAGCCCCATTTTGATGACCACAAAATTTAGCCCCCATCCGCCCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAAc < 1:18668/151‑1 (MQ=255) aCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACt > 2:864646/1‑151 (MQ=255) ccAGCATCGGCGGGGGCATTTTATGAAGCCCCACTTTGATGCCCCAAAAATTTAGCCCCCATACGCCCCCTACCATTAGCGCCACCCCCCCATTTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCt < 1:208724/151‑1 (MQ=255) cAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTg > 2:580856/1‑151 (MQ=255) agcacaaggggTGGCATGTTATGAAGCCCCCCTTTGATGCCCACAAAATTTAGCCCCCATACGCCCCCTACCATTAGCGCCACCCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCt < 2:66193/140‑1 (MQ=255) aGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTg > 1:786036/1‑150 (MQ=255) aTCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAt < 1:30752/150‑1 (MQ=255) aTCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAt < 1:436304/150‑1 (MQ=255) aTCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCAATACGCCCACCACCAGTAGCGCCAACACCCCCTCTTTTCGCGACATTTTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAtt < 1:649637/151‑1 (MQ=255) cAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCAACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTa < 1:622974/151‑1 (MQ=255) cAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAttt < 1:747046/150‑1 (MQ=255) gCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACt > 1:49703/1‑151 (MQ=255) cGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACtt > 2:433118/1‑151 (MQ=255) gTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACtttt < 1:732996/151‑1 (MQ=255) attttATGAGCCCCCACTTTGATGACCAAAAAATTTACCCCCCATACCCCCCCTACCATTAGCGCCAACCCCCCATCTTTCCACGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCttt < 2:775816/147‑1 (MQ=255) agcccccGCTTTAAGGACCACAAATTTTAGCCCCCATACGCCCCCTTCCATTCGCGCCAACCCCCCATCTTTTCGCGCCATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGtt < 2:495857/146‑1 (MQ=255) aaGCCCCTTTTTGATTCCCACAAAATTTAGCCCCCAAACGCCCCCAACCAGTAGCCCCAACCCCCCATCTTTTCGCAACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGttt < 2:636480/150‑2 (MQ=255) atgaCCACAAAATTTAGCCCCCATCCGCCCCCTCCCATTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGcc < 2:308122/151‑1 (MQ=255) tgaCCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCg > 1:626636/1‑151 (MQ=255) aaaTTTAGCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTacgac < 1:859041/151‑1 (MQ=255) atttgaccccccATACTACCCCCCCCAGTAGCCCCAACCCCCCCTCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTacgacg < 2:685267/144‑1 (MQ=255) gCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCAtt > 1:340972/1‑150 (MQ=255) ccccATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGCAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAAt > 1:546080/1‑151 (MQ=255) ccATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgc < 1:205926/151‑1 (MQ=255) cATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgcg > 1:176569/1‑151 (MQ=255) aTACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgcgc > 1:610338/1‑151 (MQ=255) cctctcccAGTACCGCAAACGCCCCTTCTTTTCCCGGTTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAAt < 2:708661/143‑1 (MQ=255) aCTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGt > 2:293489/1‑151 (MQ=255) ccAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCt < 1:788802/151‑1 (MQ=255) ccAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCt > 2:471279/1‑151 (MQ=255) aGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATAc < 2:69896/150‑1 (MQ=255) aGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACt > 1:113045/1‑151 (MQ=255) cgcCACCCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACtt < 1:662077/150‑1 (MQ=255) ccccAACCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACt < 2:478740/147‑1 (MQ=255) cacCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAttt < 2:462388/151‑1 (MQ=255) | TACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTT > NC_000913/1620970‑1621267 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |