Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,701,303	0	T	C	45.0%	74.9 / 18.2	61	I116T (ATC→ACC)	malY	PLP‑dependent beta‑cystathionase and maltose regulon regulator
Reads supporting (aligned to +/- strand):  ref base T (25/8);  new base C (0/27);  total (25/36)
Fisher's exact test for biased strand distribution p-value = 4.48e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAA  >  NC_000913/1701155‑1701448                                                                                                                                                      |                                                                                                                                                  gATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATcc                                                                                                                                                 >  1:29960/1‑151 (MQ=255)    gAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCcaca                                                                                                                                              <  2:211471/151‑1 (MQ=255)     aGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCcacac                                                                                                                                             >  1:482490/1‑151 (MQ=255)      gTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCcacac                                                                                                                                             >  1:457874/1‑150 (MQ=255)            cgcgGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGc                                                                                                                                       <  1:499406/150‑1 (MQ=255)             gcgGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCt                                                                                                                                     >  2:558772/1‑151 (MQ=255)                             ggTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACa                                                                                                                      >  2:471233/1‑150 (MQ=255)                                     aCCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTg                                                                                                             >  2:800599/1‑151 (MQ=255)                                     aCCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTg                                                                                                             >  1:236456/1‑151 (MQ=255)                                      cccgccATTCCCCCCCCATCTTTTCTCAGCGGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGACCTGTTTCGCAAGTGGTCTAAAACGGGGGAGGGCGTGGTGACCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGa                                                                                                            <  2:430730/146‑1 (MQ=255)                                      cccAGATTTACACCGCCATGGATTCTCAGAGGGGGGGGTATGTCCCTTGTGTATATTATAGGGTTAA‑AGAACTTTTTCGTCGGTGGTCGAAAACAGGTGAAGGCTGGGTGCCCCCCCCCCCCGCCTATGACGCATTTTACAAGGCCATTGa                                                                                                            <  1:282332/151‑1 (MQ=255)                                         aGCTTTACCCCGCCATCGTTTCTCGGTGGGGGGTTTATGGCCCTTCTGTCATCTATATGTTTTC‑AGAACTGATTCGTCAGGGGTCTGAAACAGGGGAGGGCGTGGTAATCCACACCCCCGCCAATGACGCATTTTACAAGGCCATTGAAgg                                                                                                         <  2:5598/151‑1 (MQ=255)                                                ccccGCCATCGTTTCTCGGGGGGTGGTGTAGGGCCTTTCTTTCATCTATATGGTGAC‑AGACCTGATCCGACGTTGTTCGGAAACAGGTGAAGGCGTGGTAACCCACACGCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTaa                                                                                                      <  1:401413/145‑1 (MQ=255)                                                 acCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAgc                                                                                                 >  1:151802/1‑151 (MQ=255)                                                   cGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAgcgc                                                                                               >  2:236470/1‑151 (MQ=255)                                                    gCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAATCAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAgcgc                                                                                               >  2:579236/1‑150 (MQ=255)                                                    gCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAgcgc                                                                                               >  1:638108/1‑150 (MQ=255)                                                      caccgtttCCCAGACGGTGGTGTATGCCCCTTCTGTCATCTATATGGTTCC‑AGACCTGATTCGTCTGTGGCCAGAAACAGGTGAAGCCGTGGGGACCCACCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGca                                                                                              <  2:15478/143‑1 (MQ=255)                                                            ttCTCGGACGGTGGGGTATGCCCCTTCTTTCATCTATATGGTTTC‑AGAACTGGTTCATCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCATACACCTGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATg                                                                                      >  2:878084/1‑151 (MQ=255)                                                            ttCTCAGAGGGTGGTGTATGGCCCTTCTGTAATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTAAAACAGGTGAGGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTaa                                                                                        <  1:41342/149‑1 (MQ=255)                                                            ttCTCAGACGGTGGTGTATGCCCCTTCTGTCATTTATAGGGTTAC‑AGATCTGATTCGTCAGTGGTCTAAAACGGGTGAAGGCGTGGTGACCCACACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGt                                                                                          <  1:643586/147‑1 (MQ=255)                                                             tctcAGGGGGTGGTGTGGGGCCTTTCTGTCATCTATAGGTTTTC‑AGAACTGATTCGTAGGTGGTCAAAAACAGGTGAAGGCGGGGTACCCCACCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATg                                                                                      <  2:646003/150‑1 (MQ=255)                                                                cAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCg                                                                                  >  2:266465/1‑151 (MQ=255)                                                                    gggtggtGTATGCCCTTTCTGTTATATATGTGGTTAC‑AGAACCGTTACGTCAGTGGTCGCAAAAAGTTGAAGGCGTGGAGACCCCCTAGCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGc                                                                              <  1:724809/150‑1 (MQ=255)                                                                     ggtggtGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCt                                                                             >  2:445097/1‑151 (MQ=255)                                                                        ggtgTATGCCCCTTCTGTCATCTATAGGGTTTC‑AAACCTGTTTCGTCGGGGGTCTGAAACAGGTGAAGGGGTGGTGACCCCCCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTa                                                                          <  2:14142/151‑1 (MQ=255)                                                                           gtATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTag                                                                         >  1:635638/1‑149 (MQ=255)                                                                             aTGGCCTTTCTGTCATCTATATGGTTAA‑AAAACTTATTCGTCAGGGGTCTGAAACGGGTGAGGGGGGGGTGACCCACACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAgaga                                                                      <  1:619875/150‑1 (MQ=255)                                                                             aTGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAgaga                                                                      >  1:32246/1‑150 (MQ=255)                                                                              tGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAgaga                                                                      >  2:448436/1‑149 (MQ=255)                                                                                 cctttCTGTCTTTTATATGTTTTC‑AGAACTGTTTCGTCAGTGGTCTAAAACGGGTGAGGGGGTGGTGACCCCCACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAg                                                                 <  1:24295/148‑1 (MQ=255)                                                                                 cccTTCTGTCTTCTATATGTTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGACCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCa                                                                  <  1:469380/150‑1 (MQ=255)                                                                                      cTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTg                                                             >  2:4729/1‑150 (MQ=255)                                                                                          cATCTATATGGTTTC‑AGAACTGTTTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGAtgg                                                         <  1:141746/150‑1 (MQ=255)                                                                                              tataTGGTTTC‑AGAACTGATTCGTCAGCGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGAtggttggt                                                    >  2:490882/1‑151 (MQ=255)                                                                                                   gtttgc‑AGACCTGATTCGTCGGGGGTCTAAAACGGGTGAAGGGGTGGTGACCCACCCGCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGAtggttggt                                                    <  1:734968/141‑1 (MQ=255)                                                                                                   ggTTTC‑AGAACTGATTCGTCGGTGGTCTGAAACGGGTGAGGGGGGGGTAACCCACACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGc                                               <  2:489188/151‑1 (MQ=255)                                                                                                    gTTTCAAGACCTGATTCGCCGGTGGTCTGAAACGGGGAAGGGCGTGGTGACCCACCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGAtggttg                                                      <  2:264929/144‑1 (MQ=255)                                                                                                       ttaaGAACTTATTCGTCAGTGGTCTGAAACAGGGGAAGGCGTGGTGACCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGa                                             <  1:679531/147‑1 (MQ=255)                                                                                                          aaatcTGATTCGTAGGTGGGCTGAAACAGGTGAGGGCGGGGTGACCCACCCACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATg                                         <  2:179533/147‑1 (MQ=255)                                                                                                               tgtttCGTCAGTGTTCAGAAACAGGTGAAGGGGTGTTCACCCCCCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCa                                     <  2:57762/147‑1 (MQ=255)                                                                                                               tGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGcg                                     >  2:271683/1‑149 (MQ=255)                                                                                                                  ttCGTCAGTGGTCTGAAAAAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCa                                     <  1:49556/147‑1 (MQ=255)                                                                                                                          tgttCTAAAACAGGTGAGGGCGTGGTAACCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGcc                          <  2:532854/147‑1 (MQ=255)                                                                                                                          tGGTCGAAAACAGGTGATGGCGTGGAGACCCACCCACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAg                            <  2:722580/148‑1 (MQ=255)                                                                                                                           gttCTGAAACGGGTGAAGGGGTGGTGACCCCCACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGc                           <  2:265395/146‑1 (MQ=255)                                                                                                                               tGAAACAGGGGAAGGGGTGGTTCTACCCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGtt                     <  2:739532/150‑1 (MQ=255)                                                                                                                                 aaaCAGGTGAAGGCGTGGTGACCCACACCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGc                  <  2:608898/151‑1 (MQ=255)                                                                                                                                  aaCGGGTGAAGGCGTGGTGACCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCg                 <  2:564765/151‑1 (MQ=255)                                                                                                                                      ggggaggggggggtgACCCCCCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGaa               <  2:648993/138‑1 (MQ=255)                                                                                                                                       gagAAGGCGTGGTCCACCCCCCACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAAcc            <  2:766261/149‑1 (MQ=255)                                                                                                                                       gTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAAcc            >  1:278908/1‑151 (MQ=255)                                                                                                                                        tGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAAcc            >  1:46653/1‑150 (MQ=255)                                                                                                                                          aaGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAAcc            >  2:501361/1‑148 (MQ=255)                                                                                                                                            ggggtggtgACCCCCCCCCCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAAt       <  1:699523/148‑1 (MQ=255)                                                                                                                                            ggCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGaa        <  1:563371/150‑1 (MQ=255)                                                                                                                                             gggtggtgACCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATg      <  2:70671/149‑1 (MQ=255)                                                                                                                                              cGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATg      >  2:183168/1‑150 (MQ=255)                                                                                                                                               gtggtgACCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGt     <  2:542003/150‑1 (MQ=255)                                                                                                                                               gtggtgACCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTa    <  1:328408/151‑1 (MQ=255)                                                                                                                                                tggtgATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTa    >  1:412869/1‑150 (MQ=255)                                                                                                                                                 ggtcctCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTCGAAGCCGTGTTGGCGAAACCAGAATGTaaa  <  2:718161/146‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  GATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTC‑AGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAA  >  NC_000913/1701155‑1701448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑