Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA46.5% 50.7 / 23.1 43intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (22/1);  new base A (0/20);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 2.19e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAA  >  NC_000913/1824909‑1825202
                                                                                                                                                     |                                                                                                                                                  
gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTgagaagagaa                                                                                                                                                  >  2:221755/1‑150 (MQ=255)
   aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaa                                                                                                                                                 >  2:797258/1‑148 (MQ=255)
        aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAc                                                                                                                                          <  2:174866/150‑1 (MQ=255)
           cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGCTGGTTATCGGAAACTCGGGTGATTTAAG‑TTAGTGGTGTTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAActgcc                                                                                                                                      >  2:538430/1‑151 (MQ=255)
                cAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATcc                                                                                                                                 >  2:617390/1‑151 (MQ=255)
                 aaaGTATTCCATCCTCGCTCCCGGCCGGTTTTTTTCTTTGTTATCGTTCAGGTACCCGT‑GGATGTTTATCGGCAACTGGGGTGATTTAGG‑TTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCt                                                                                                                                <  2:69311/151‑1 (MQ=255)
                   aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGt                                                                                                                              >  1:631331/1‑151 (MQ=255)
                    gTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc                                                                                                                             >  1:697141/1‑151 (MQ=255)
                      aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                            >  2:608273/1‑150 (MQ=255)
                        tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                         >  1:537224/1‑151 (MQ=255)
                            tccccGCTCCCGGCCCTTTCTTTTCTTTTTTGTTGTGAAGGTACGGGG‑GGATGTTTTTGGCCAACT‑GGGTTTTTTTCGTTTGGTGGTGTTCAGGTTTTTTTTACTATTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCa                                                                                                                           <  2:520232/141‑1 (MQ=255)
                                                ttttCTTTTTTTTCGTTCAGTTACCGGT‑GGAGGTTTACCGAAACCGCGGTTGTTTTCGG‑TTGTTGGTGTTCGGGTTTTTTTTAAAGCTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                   <  1:613559/149‑1 (MQ=255)
                                                ttttCTTTTTTATCGTTCAGGTACCCGT‑GGTGGGTTATCGGCAACTCGGTTGTTTTCCG‑TTAGTGGTTATCGGTTTTTTTTTACAATTTTTAGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                  <  1:418390/150‑1 (MQ=255)
                                                    cATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                             >  1:769694/1‑151 (MQ=255)
                                                       tGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                          >  2:360582/1‑151 (MQ=255)
                                                         ttATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                        >  1:825212/1‑151 (MQ=255)
                                                             cGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                    >  2:45732/1‑151 (MQ=255)
                                                               ttCAGGTACCCGT‑GGATGGTTATCGGCAACTGGGGTTTTTTACG‑TTAGTGGTGATCAGGTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                   <  1:160389/150‑1 (MQ=255)
                                                                tccgggaaCCGTGGGATGTTTACCGCCACCTGGGTTGTTTTACT‑TTGGGGGTGACCGGGTTTTTTTTACATTTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt                                                                                     <  2:561562/142‑1 (MQ=255)
                                                                  aGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                               >  1:604412/1‑151 (MQ=255)
                                                                   ggTACCGGT‑GGATGGTTATCGGCAACTGGGGTGATTTACG‑TTAGTGGTGATCAGGTTTTTTTTAAAACTGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                               <  1:200074/150‑1 (MQ=255)
                                                                    ggaaacctgggATGGTTAGGGGAACCCGGGGTGATTTCCG‑TTAGGGGTGACCGGTTTTTTTTTACAATTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                               <  1:629248/141‑1 (MQ=255)
                                                                     tAACCGC‑GGATGGTTATCGGCAACTCGGGGGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAAGACCGGCGGTCTTTAGttt                                                                              >  2:356847/1‑149 (MQ=255)
                                                                        ccGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGgttttt                                                                           >  1:68772/1‑149 (MQ=255)
                                                                           t‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCt                                                                       >  2:485103/1‑150 (MQ=255)
                                                                             ggATGTTTATGGGAAACGGGGGTAATTTACG‑TTAGTGGTGATCAGTTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg                                                                      <  2:258461/150‑1 (MQ=255)
                                                                             ggATGGTTATCGGCACCTGGGGTGTTTTACG‑TTAGTGGTGATCAGGTTTTTTTTACACCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                        <  1:224140/148‑1 (MQ=255)
                                                                               aTGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                    >  1:561092/1‑150 (MQ=255)
                                                                                   ttAGCGGCAACTGGGGTGATTTACG‑TTAGTGGTGATCGGTTTTTTTTTACAAGTGTTAAAGAAAAAAAAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                                <  2:246122/150‑1 (MQ=255)
                                                                                        ggCAACTGGGGTGTTTTAGT‑TTAGTGGTGATCGGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                          <  1:797335/151‑1 (MQ=255)
                                                                                           aaCTGGGTTAATTTACG‑TTAGTGGTGATCAGTTTTTTTTTACACCTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                            <  2:650581/146‑1 (MQ=255)
                                                                                           aaCTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                        >  1:374487/1‑150 (MQ=255)
                                                                                              tCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                    >  2:39428/1‑151 (MQ=255)
                                                                                                  gTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                  >  1:851860/1‑149 (MQ=255)
                                                                                                           g‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                       >  1:280540/1‑151 (MQ=255)
                                                                                                             ttAGTGGTGATCGGTTTTTTTTTAAACCTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                          <  2:458311/147‑1 (MQ=255)
                                                                                                                ggggtgATCGGTTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                        <  2:153276/144‑1 (MQ=255)
                                                                                                                  ggttacgagtttttttttACAACTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                  <  2:144364/139‑1 (MQ=255)
                                                                                                                        cAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTTGCTGAAAAAATATTTAGCAGTTGAAGGCATTTTACCTTTTGCCGCTg                           >  2:562274/1‑151 (MQ=255)
                                                                                                                          ggttttttttACAATTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                          <  1:168043/147‑1 (MQ=255)
                                                                                                                             tttttttCCCTCTTTTTGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGa                         <  2:276519/148‑1 (MQ=255)
                                                                                                                                ttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAATTTTTTGGCTGAAAGATTTTTTAACAGGTGCAGGCATTTTACCCTTTTCCCCTGGACGtt                     >  2:485891/1‑149 (MQ=255)
                                                                                                                                      aaCTGTTGAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa             <  2:166991/151‑1 (MQ=255)
                                                                                                                                        cTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAg              >  1:25207/1‑148 (MQ=255)
                                                                                                                                            ttGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt        <  1:219226/150‑1 (MQ=255)
                                                                                                                                                  agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa  >  1:363972/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAA  >  NC_000913/1824909‑1825202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑