Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA43.2% 57.9 / 23.0 44intergenic (‑124/+79)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (23/2);  new base A (0/19);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.49e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT  >  NC_000913/1824917‑1825211
                                                                                                                                                  |                                                                                                                                                      
aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAc                                                                                                                                                   <  2:174866/150‑1 (MQ=255)
   cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGCTGGTTATCGGAAACTCGGGTGATTTAAG‑TTAGTGGTGTTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAActgcc                                                                                                                                               >  2:538430/1‑151 (MQ=255)
        cAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATcc                                                                                                                                          >  2:617390/1‑151 (MQ=255)
         aaaGTATTCCATCCTCGCTCCCGGCCGGTTTTTTTCTTTGTTATCGTTCAGGTACCCGT‑GGATGTTTATCGGCAACTGGGGTGATTTAGG‑TTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCt                                                                                                                                         <  2:69311/151‑1 (MQ=255)
           aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGt                                                                                                                                       >  1:631331/1‑151 (MQ=255)
            gTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc                                                                                                                                      >  1:697141/1‑151 (MQ=255)
              aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                                     >  2:608273/1‑150 (MQ=255)
                tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                                  >  1:537224/1‑151 (MQ=255)
                    tccccGCTCCCGGCCCTTTCTTTTCTTTTTTGTTGTGAAGGTACGGGG‑GGATGTTTTTGGCCAACT‑GGGTTTTTTTCGTTTGGTGGTGTTCAGGTTTTTTTTACTATTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCa                                                                                                                                    <  2:520232/141‑1 (MQ=255)
                                        ttttCTTTTTTTTCGTTCAGTTACCGGT‑GGAGGTTTACCGAAACCGCGGTTGTTTTCGG‑TTGTTGGTGTTCGGGTTTTTTTTAAAGCTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                            <  1:613559/149‑1 (MQ=255)
                                        ttttCTTTTTTATCGTTCAGGTACCCGT‑GGTGGGTTATCGGCAACTCGGTTGTTTTCCG‑TTAGTGGTTATCGGTTTTTTTTTACAATTTTTAGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                           <  1:418390/150‑1 (MQ=255)
                                            cATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                      >  1:769694/1‑151 (MQ=255)
                                               tGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                                   >  2:360582/1‑151 (MQ=255)
                                                 ttATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                                 >  1:825212/1‑151 (MQ=255)
                                                     cGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                             >  2:45732/1‑151 (MQ=255)
                                                       ttCAGGTACCCGT‑GGATGGTTATCGGCAACTGGGGTTTTTTACG‑TTAGTGGTGATCAGGTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                            <  1:160389/150‑1 (MQ=255)
                                                        tccgggaaCCGTGGGATGTTTACCGCCACCTGGGTTGTTTTACT‑TTGGGGGTGACCGGGTTTTTTTTACATTTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt                                                                                              <  2:561562/142‑1 (MQ=255)
                                                          aGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                        >  1:604412/1‑151 (MQ=255)
                                                           ggTACCGGT‑GGATGGTTATCGGCAACTGGGGTGATTTACG‑TTAGTGGTGATCAGGTTTTTTTTAAAACTGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                        <  1:200074/150‑1 (MQ=255)
                                                            ggaaacctgggATGGTTAGGGGAACCCGGGGTGATTTCCG‑TTAGGGGTGACCGGTTTTTTTTTACAATTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                        <  1:629248/141‑1 (MQ=255)
                                                             tAACCGC‑GGATGGTTATCGGCAACTCGGGGGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAAGACCGGCGGTCTTTAGttt                                                                                       >  2:356847/1‑149 (MQ=255)
                                                                ccGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGgttttt                                                                                    >  1:68772/1‑149 (MQ=255)
                                                                   t‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCt                                                                                >  2:485103/1‑150 (MQ=255)
                                                                     ggATGTTTATGGGAAACGGGGGTAATTTACG‑TTAGTGGTGATCAGTTTTTTTTTACAATTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg                                                                               <  2:258461/150‑1 (MQ=255)
                                                                     ggATGGTTATCGGCACCTGGGGTGTTTTACG‑TTAGTGGTGATCAGGTTTTTTTTACACCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                                 <  1:224140/148‑1 (MQ=255)
                                                                       aTGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                             >  1:561092/1‑150 (MQ=255)
                                                                           ttAGCGGCAACTGGGGTGATTTACG‑TTAGTGGTGATCGGTTTTTTTTTACAAGTGTTAAAGAAAAAAAAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                                         <  2:246122/150‑1 (MQ=255)
                                                                                ggCAACTGGGGTGTTTTAGT‑TTAGTGGTGATCGGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                                   <  1:797335/151‑1 (MQ=255)
                                                                                   aaCTGGGTTAATTTACG‑TTAGTGGTGATCAGTTTTTTTTTACACCTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                                     <  2:650581/146‑1 (MQ=255)
                                                                                   aaCTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                                 >  1:374487/1‑150 (MQ=255)
                                                                                      tCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                             >  2:39428/1‑151 (MQ=255)
                                                                                          gTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                           >  1:851860/1‑149 (MQ=255)
                                                                                                   g‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                                >  1:280540/1‑151 (MQ=255)
                                                                                                     ttAGTGGTGATCGGTTTTTTTTTAAACCTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                                   <  2:458311/147‑1 (MQ=255)
                                                                                                        ggggtgATCGGTTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                                 <  2:153276/144‑1 (MQ=255)
                                                                                                          ggttacgagtttttttttACAACTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                           <  2:144364/139‑1 (MQ=255)
                                                                                                                cAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTTGCTGAAAAAATATTTAGCAGTTGAAGGCATTTTACCTTTTGCCGCTg                                    >  2:562274/1‑151 (MQ=255)
                                                                                                                  ggttttttttACAATTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                                   <  1:168043/147‑1 (MQ=255)
                                                                                                                     tttttttCCCTCTTTTTGAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGa                                  <  2:276519/148‑1 (MQ=255)
                                                                                                                        ttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAATTTTTTGGCTGAAAGATTTTTTAACAGGTGCAGGCATTTTACCCTTTTCCCCTGGACGtt                              >  2:485891/1‑149 (MQ=255)
                                                                                                                              aaCTGTTGAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa                      <  2:166991/151‑1 (MQ=255)
                                                                                                                                cTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAg                       >  1:25207/1‑148 (MQ=255)
                                                                                                                                    ttGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt                 <  1:219226/150‑1 (MQ=255)
                                                                                                                                          agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa           >  1:363972/1‑150 (MQ=255)
                                                                                                                                            aaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaaa          <  2:475255/147‑1 (MQ=255)
                                                                                                                                               aaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGGATTTTTCCTTTTGCCGCTGGACGTTCTGTCAGACGCGTCTCAAAAATAg      >  2:874735/1‑150 (MQ=255)
                                                                                                                                                  gaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCAtt  >  1:304294/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
ACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGT‑GGATGGTTATCGGCAACTCGGGTGATTTACG‑TTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT  >  NC_000913/1824917‑1825211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑