Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4860CA43.6% 84.3 / 33.8 55intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (24/7);  new base A (0/24);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.11e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29338‑29633
                                                                                                                                                        |                                                                                                                                                   
tttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCcaca                                                                                                                                                    <  1:14963/150‑1 (MQ=255)
  tgttgtTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACaaa                                                                                                                                                  <  2:462629/150‑1 (MQ=255)
   gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACaaaa                                                                                                                                                 <  2:676379/150‑1 (MQ=255)
   gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaat                                                                                                                                                >  1:686935/1‑151 (MQ=255)
    ttgttTTAATGTAAATTTTGACCTTTTGGTCCACTTTTTTCTGCTCTTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGCTCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGCGCCCCAAaat                                                                                                                                                <  2:806572/150‑1 (MQ=255)
    ttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaat                                                                                                                                                <  2:549072/150‑1 (MQ=255)
      gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaataat                                                                                                                                             <  2:111681/151‑1 (MQ=255)
           aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCTTTTTTATTTCATGCAATCTTCTTGCTGCCCAAGCGTTTCCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGACCCACAAAATAATATaat                                                                                                                                        <  2:187112/151‑2 (MQ=255)
              gttaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                      >  1:657746/4‑150 (MQ=255)
               tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                    >  2:773162/1‑151 (MQ=255)
                   ttttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCg                                                                                                                                >  2:880846/1‑151 (MQ=255)
                       gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                            >  1:40347/1‑151 (MQ=255)
                        aCCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                           >  2:15010/1‑151 (MQ=255)
                              tGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                     >  2:631693/1‑151 (MQ=255)
                                gTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGAc                                                                                                                   >  1:439322/1‑151 (MQ=255)
                                gTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGAc                                                                                                                   >  1:816724/1‑151 (MQ=255)
                                     tttttttCTGCTCTTTTTTATTCATTGCATTCTTCTTGTTGCACAAGCTTTTTCAAAAACAGGTTAGATGATTTTTTTGCCTTTTAATGCCTGAAAAACATCCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                               <  1:58940/149‑1 (MQ=255)
                                              gCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                      >  2:146073/1‑150 (MQ=255)
                                                     ttttttacttGCAATCTTCTTCCGACGAAGGCTTTTCCAAAATCAGTTAAGAGTATTTTTTTTTCATTTAATCCTGTAAAAAATTGATTGACCCCAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                               <  2:689863/141‑1 (MQ=255)
                                                      ttATTTCATGCATTTTTTTTCCTCCGAAACCGTTTCCCAGAAAAGGTTATATTATTTTTTTGTCCCTAATTCCCTGAAAAACTTGCATGAGCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                              <  2:811531/150‑1 (MQ=255)
                                                            cATGCATTCTTCTTGCTGCGCAAGCTTTTCCCAGAACAGGTTAGATGATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                         <  1:68463/149‑1 (MQ=255)
                                                              tGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                     >  1:394749/1‑151 (MQ=255)
                                                                 aaTCTTCTTGCTGCGCAAGCTTTTTCAAGAACAGGTTAGATGATTTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                     <  1:550360/148‑1 (MQ=255)
                                                                  aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                 >  1:472859/1‑151 (MQ=255)
                                                                     ttcttGTTGCACAGGCTTTTCCAAGAACGGTTTAAATTATTTTTTTGCCTCTAAATACTTTAAAAACATGAAGGGCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                  <  1:522495/147‑1 (MQ=255)
                                                                     ttcttGCTGCGCAAGCTTTTTCAAGAATAGTTAAGTTTATTTTTTTGTCGTTAAATGCCTGAAAAACATGCAGGAGCCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                       <  1:501493/142‑1 (MQ=255)
                                                                      tcttGCTGCGAAAGCTTTTCCAAAAACGGTTAAAATTTTTTTTTTTTCTTTTAATGCTTAAAAAACATGAATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                <  2:819956/148‑1 (MQ=255)
                                                                        ttcctgcGCAAGCTTTTCCCAAAACAGGTTAGATGATTTTTTTGTCGTTTAATCCTTGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                               <  1:809194/144‑1 (MQ=255)
                                                                           ctgcACAAGCTTTTCCCAGAACGGTTAAGATGATTTTTTTGTCGTTAAATGCCTGAAAAACATCCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCa                                                                             <  1:843467/146‑1 (MQ=255)
                                                                            tgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                       >  1:449547/1‑151 (MQ=255)
                                                                             gcgcAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                      >  1:494663/1‑151 (MQ=255)
                                                                              cgaaaGCTTTTTCAAAAACAGGTTAGATGATTTTTTTTTCGTTTAATGCTTAAAAAACATGCATGAGCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                        <  2:381053/145‑1 (MQ=255)
                                                                                 aggctttttCAAAAACAGGTTAGATTTTTTTTTTGTCTTTTAATCCTTAAAAAACATGAATGACCAAAAAAAAAAAAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                   <  2:570214/145‑1 (MQ=255)
                                                                                      ttttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                               >  1:859010/1‑149 (MQ=255)
                                                                                       tttCCAAGAACAGGTAAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCAGGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                              <  1:461663/150‑1 (MQ=255)
                                                                                       tttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAg                                                             >  1:193240/1‑150 (MQ=255)
                                                                                          tcccaaaaagggTTAGATGATTTTTTTGTCGCTTAATGCTTAAAAAAAATGAAGGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                            <  1:278690/140‑1 (MQ=255)
                                                                                             aGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                       >  2:480255/1‑151 (MQ=255)
                                                                                                aCAGTTTAGATGTTTTTTTTGTCTTTTAAGGCCTGAAAAACATGAATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                    <  1:861089/151‑1 (MQ=255)
                                                                                                   aGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                  >  2:46129/1‑151 (MQ=255)
                                                                                                            tgttttttttCTCGCTAAATCCTTTAAAAACAGGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                              <  2:664897/142‑1 (MQ=255)
                                                                                                               ttttttttTCGTTTAAGCCCTAAAAAACATGCATGACCCAAAAAATAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                         <  2:449561/147‑1 (MQ=255)
                                                                                                                cTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                       >  1:519084/1‑150 (MQ=255)
                                                                                                                cTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                       >  2:566640/1‑150 (MQ=255)
                                                                                                                 ttttttTCCTTTAATCCCTTAAAAACTTGCATGCCCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                        <  2:33467/148‑1 (MQ=255)
                                                                                                                  ttttttCGCTTAATCCCTATAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                       <  1:189468/148‑1 (MQ=255)
                                                                                                                      gTCGTTTAAGGCCTGTAAAACATGCAGGAGCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                 <  2:137151/150‑1 (MQ=255)
                                                                                                                      gTCACTTAATGCCTGAAAAACTTGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                  <  1:551707/149‑1 (MQ=255)
                                                                                                                               tGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                       >  1:477321/1‑151 (MQ=255)
                                                                                                                                gCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                       >  1:751930/1‑150 (MQ=255)
                                                                                                                                      aaaaCATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                 <  1:862221/150‑1 (MQ=255)
                                                                                                                                         aCATGCATGACCAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa             <  1:443802/151‑1 (MQ=255)
                                                                                                                                             tgcaggAACCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa              <  2:157754/147‑1 (MQ=255)
                                                                                                                                                atgACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa            <  1:752781/146‑1 (MQ=255)
                                                                                                                                                   aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg     >  2:227492/1‑150 (MQ=255)
                                                                                                                                                     ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat  >  1:594779/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                   
TTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29338‑29633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: