Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 907,602 | 0 | A | C | 44.8% | 82.1 / 15.0 | 59 | G227G (GGT→GGG) | ybjT | putative NAD‑dependent oxidoreductase |
Reads supporting (aligned to +/- strand): ref base A (24/8); new base C (0/26); total (24/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.40e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGCG > NC_000913/907460‑907751 | cgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCAc < 2:420790/151‑1 (MQ=255) catcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCa < 1:30934/150‑1 (MQ=255) catcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCa < 1:840836/150‑1 (MQ=255) ccGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGGGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAACCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCAt < 2:187403/148‑1 (MQ=255) cGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATaaa > 2:838359/1‑150 (MQ=255) agATCGCGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGGGGGCGGTTCGGAAGAAATCACATTGAGAAACCACACCGAAATCCAGCGGGAGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCa > 2:356199/1‑150 (MQ=255) gtTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTg > 1:627440/1‑151 (MQ=255) ttccAGCCCCTGAACCACCGCCCGGGGGGTGGTGGGGGGTACGAAATAAATCACATTGAAAACCCACCCCAAAACCCAGGGGGGGGGGAGGGGGTGGGGGAT‑CACCCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTg < 2:435584/147‑1 (MQ=255) ccccTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATa > 1:507902/1‑150 (MQ=255) gAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTg > 2:873476/1‑149 (MQ=255) accAACCCCCTGGCGGTGGTGGGGGGTACGAAAGTAACCACATAAAAAACCCCCACCAAAATCGAGGGGGTGGGGGGGGGGATGGGAAT‑CAACGGGCGGCGCTTCCCTCTGTCCGCCATAAAATGTTCAAACTGTTGCTGATAACt < 1:96956/144‑1 (MQ=255) aaCGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCAcc > 1:103671/1‑150 (MQ=255) cGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACctct > 1:400953/1‑151 (MQ=255) cGCCCGGGGGGTGGGGGGGGGAAGGAAATAAATCACATAAAGAACCCCCCCCGAAACCCGGGGGGTGGGGGGGGGGAGGGGGAT‑AACCCGGCGGCCCTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACctct < 2:838007/151‑1 (MQ=255) gggggtgggGGGCGGTAGGAAAGTAATCACAGTGAAAAACCACCCCAAAACCCGGCGGGGGGGGGGGGGGAGGGGGAT‑CAACCAGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTcc < 1:824243/147‑1 (MQ=255) gtggtgGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggcgg > 1:861732/1‑151 (MQ=255) gggggggggggTAGGAAAGTAATCACATTGAGAACCCCCCCCAAAACCCGGGGGGGGGGGGGGGGGAGGGGGAT‑CACCCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggcgg < 2:788412/146‑1 (MQ=255) tggtggGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCggcggc > 1:680263/1‑151 (MQ=255) ggtggGGGGTACGAAAGTAATCACATTGAGAACCCCCACCGAAACCCGGGGGGGGGGGGGGGGGAGGGGAAT‑CACCCGGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCt < 1:692611/151‑1 (MQ=255) ggggggCGGTACGGAAGAAACCACATTGAAAACCCCCACCAAACTCCGGGGGGGGGGGGGGGGGTGGGGAAT‑CACCCAGCGGCGCTCACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCt < 2:832638/148‑1 (MQ=255) gtggGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCtt > 1:472710/1‑151 (MQ=255) ggggCGGTAGGGAAGAAATCACATTGAAAACCCACACCGAAACCCGGGGGGGGGGGGGGGGGAGGGGGAT‑CAACCGGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTc < 1:586052/150‑1 (MQ=255) gTAGGGAAGAAATCAGATGAAAACACCACACCAAATTCCAGGGGGTGGGGAGGGGGAGGGGAGT‑CAACAGGCGGCGCTCACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGaa < 2:504113/148‑1 (MQ=255) ggggaaaatAACCACATGGAAAACCCCCCCCAAAGCCCAGGGGGGGGGGGGGGGGATGGGGAT‑AACCGGGCGGCTCTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATg < 2:507846/143‑1 (MQ=255) aCGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGc > 1:850578/1‑151 (MQ=255) gtaaaGAAATCACTATAAAAAACCACGCCTAAACCCAGGGGATGGGGGGGGGTGAGGGTAT‑CAACGTGTGTCTTTCTCCCCTCACCGCCATAAAATGTTCAAACAGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg < 2:19962/148‑1 (MQ=255) ggAAGTAACCACATTGAGAACCCCCCCCAAAACCCGGGGGGGGGGGGGGGGGATGGGGAT‑CACCCGGCGGCTCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATg < 2:379639/148‑1 (MQ=255) agtacaTCACATGGAGAACCCCCCCGGAAAACGGGGGGGGGGGGGGGGGGGAGGAGAC‑CACCCAGCGGCGCGTCCCTCACCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGa < 2:727626/136‑1 (MQ=255) aaatAATCAAATTGAAAACCCCCCCCAAAACCCGGGGGGTGGGGAGGGGGAGGGGGCT‑CACCCGGCGGCTCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGgtg < 2:604218/148‑1 (MQ=255) aaGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGgtg > 1:524776/1‑151 (MQ=255) aGTAATCACATGGAAAAACCACCCCAAAATCCGGGGGGGGGGGGGGGGGAGGGGAAT‑CAACCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGgtgt < 1:161270/151‑1 (MQ=255) gTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGtt > 2:620398/1‑151 (MQ=255) tGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCgg > 2:479039/1‑151 (MQ=255) ggacaaaCCCCCCCGAACTCCGGCGGGGGGGGAGGGGGATGGGGAT‑CACCCAGGGGCTCTCCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGcc < 2:231539/145‑1 (MQ=255) aaaaaCCCCACCAAACCCCAGGGGGGGGGGGGGGGGTGGGGGAT‑CACCCGGGGCCGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCt < 2:223706/141‑1 (MQ=255) aaaaaCCCCACCAAAAACCGGGGGGGGGGGGGGGGGAGGGGGAT‑CAACCGGGGGCGCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGgat < 2:452155/149‑1 (MQ=255) ccccaaaccccggggggTGGGGGGGGGGAGGGGGAT‑CAACCGGCGGCGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaa < 1:434102/136‑1 (MQ=255) cacCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGGGTTCGCTGGCCGGATGATCTa > 2:749596/1‑149 (MQ=255) agaccgggggggTGGGGAGGGGGTGGGGAAA‑CAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaacaac < 1:558989/140‑1 (MQ=255) aaccccAGGGGGGGGGGAGGGGGAGGGGGAC‑AACCCGGCGGCTCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACg < 1:228276/146‑1 (MQ=255) aaaCCCAGCGGGGGGGGAGGGGGA‑GGGGATACAACCAGCGGCTCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaa < 1:604940/145‑1 (MQ=255) aTCCAGGGGGTGGGGGGGGGGATGGGGAT‑CAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCaa < 1:773267/151‑1 (MQ=255) ccggggggTGGGGGGGGGGAGGGGGAT‑CAACCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAc < 1:834910/145‑1 (MQ=255) ccAGGGGGGGGGGAGGGGGATGGGGAT‑CAACCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCa < 1:218568/148‑1 (MQ=255) cAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAcc > 2:568981/1‑150 (MQ=255) ggggggggggGTAGGGGGAC‑CACCGAGCGGGTTTTCCCTCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCGACCAg < 2:95892/144‑1 (MQ=255) ggagtggggac‑CACCCGGGGGCCCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGt < 1:236150/147‑1 (MQ=255) gggtgggaat‑CACCCGGCGGCTTTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGtt < 1:146521/148‑1 (MQ=255) gatggggat‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGttt > 1:604577/1‑151 (MQ=255) tggggat‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTc > 1:511369/1‑151 (MQ=255) gggtat‑CACCCGGCGCCGTTTCCCCCCCTCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTcc < 2:623012/151‑1 (MQ=255) gggaaacacccggcggcgCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTcc < 2:641203/139‑1 (MQ=255) at‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAgcg > 1:493582/1‑151 (MQ=255) accccAGCGGCGCTACCCTCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAgcg < 1:93695/146‑1 (MQ=255) aaCCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCa > 1:346285/1‑150 (MQ=255) aCCAACGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAAt > 2:789222/1‑151 (MQ=255) gcgcTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCgtg > 1:64600/1‑151 (MQ=255) gcgcTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAACACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCgtg > 1:507936/1‑151 (MQ=255) tACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGCg > 1:425558/1‑151 (MQ=255) | CGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGAT‑CAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGCG > NC_000913/907460‑907751 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |