Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	962,056	G→T	G21V (GGT→GTT)	rpsA →	30S ribosomal subunit protein S1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	962,056	0	G	T	100.0%	203.1 / NA	54	G21V (GGT→GTT)	rpsA	30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/24);  total (30/24)

TTGCAATGGAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGGTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGAC  >  NC_000913/961909‑962201                                                                                                                                                    |                                                                                                                                                  ttGCAATGGAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTc                                                                                                                                                >  2:834756/1‑151 (MQ=255)    cAATGGAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAt                                                                                                                                             >  1:475746/1‑151 (MQ=255)         gAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttc                                                                                                                                        <  1:763390/151‑1 (MQ=255)          aaTGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttcg                                                                                                                                       >  2:814104/1‑151 (MQ=255)          aaTGACAGCGGGCATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAAACTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttcg                                                                                                                                       >  2:376330/1‑151 (MQ=255)           aTGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGGGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttcgt                                                                                                                                      <  1:386443/151‑1 (MQ=255)           aTGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTAtcgttcgt                                                                                                                                      <  1:355308/151‑1 (MQ=255)                aGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCgt                                                                                                                                 >  2:692141/1‑151 (MQ=255)                   gggTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCgttgt                                                                                                                              <  2:582175/151‑1 (MQ=255)                            aaaCAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTAt                                                                                                                     >  1:283004/1‑151 (MQ=255)                             aacaacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATc                                                                                                                    >  2:507722/1‑151 (MQ=255)                             aacaacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATc                                                                                                                    >  1:373201/1‑151 (MQ=255)                             aacaacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATc                                                                                                                    <  1:661936/151‑1 (MQ=255)                             aacaacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATc                                                                                                                    <  1:487796/151‑1 (MQ=255)                               caacCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGa                                                                                                                  <  2:445479/151‑1 (MQ=255)                                      aTCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGAc                                                                                                           >  2:785371/1‑151 (MQ=255)                                                gAGCCAGGTGGACGTTAAAAATAAACCTGAAGATTAAACATTACAGAATCTTTTGCTCAACTTTTTGAAGAGTCCTTAAAAGAAACCGACACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTgg                                                                                                 <  2:563541/151‑1 (MQ=255)                                                     aGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGAc                                                                                            <  1:381845/151‑1 (MQ=255)                                                      ggTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACg                                                                                           <  2:70937/151‑1 (MQ=255)                                                         ggACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTg                                                                                        >  1:433984/1‑151 (MQ=255)                                                          gACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTgg                                                                                       >  2:366169/1‑151 (MQ=255)                                                                  atatAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAAt                                                                               <  1:423221/151‑1 (MQ=255)                                                                        aCCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGt                                                                         >  2:514545/1‑151 (MQ=255)                                                                          cTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTcc                                                                       >  2:472472/1‑151 (MQ=255)                                                                             aaGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGcc                                                                    >  1:776936/1‑151 (MQ=255)                                                                              aGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCa                                                                   <  2:341361/151‑1 (MQ=255)                                                                                  tAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATccc                                                               >  2:750462/1‑151 (MQ=255)                                                                                   aaaCATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATccc                                                               <  1:395799/150‑1 (MQ=255)                                                                                      cATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCt                                                           >  2:694923/1‑151 (MQ=255)                                                                                       aTGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTg                                                          <  2:44092/151‑1 (MQ=255)                                                                                         gACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGa                                                         <  1:365862/150‑1 (MQ=255)                                                                                              aaTCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGtt                                                   >  2:499274/1‑151 (MQ=255)                                                                                                    ttGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCaaaa                                              <  1:252881/150‑1 (MQ=255)                                                                                                     tGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAAc                                            >  1:408076/1‑151 (MQ=255)                                                                                                         cAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGccc                                        >  2:774372/1‑151 (MQ=255)                                                                                                          aaCTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGccc                                        >  1:398179/1‑150 (MQ=255)                                                                                                           aCTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAg                                      <  2:553309/151‑1 (MQ=255)                                                                                                            ctctTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAgg                                     >  2:20762/1‑151 (MQ=255)                                                                                                              ctTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAgg                                     <  1:69155/149‑1 (MQ=255)                                                                                                                  gAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGc                               <  2:270942/151‑1 (MQ=255)                                                                                                                      agTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGAAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTgg                            >  1:583312/1‑150 (MQ=255)                                                                                                                       gTCCTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGaa                          <  2:123001/151‑1 (MQ=255)                                                                                                                         ccTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAAt                        >  1:161199/1‑151 (MQ=255)                                                                                                                         ccTTAAAAGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAAt                        >  1:383669/1‑151 (MQ=255)                                                                                                                             aaaaGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATcc                      >  1:833486/1‑149 (MQ=255)                                                                                                                             aaaaGAAATCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCag                    <  2:783116/151‑1 (MQ=255)                                                                                                                                  aaaTCGAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCaggtagg               <  1:157639/151‑1 (MQ=255)                                                                                                                                       gAAACCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACg          >  2:747301/1‑151 (MQ=255)                                                                                                                                        aaaCCCGCCCGGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGa         <  2:205280/151‑1 (MQ=255)                                                                                                                                            ccgcccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGtt     >  1:87197/1‑151 (MQ=255)                                                                                                                                              gcccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTg    >  2:90710/1‑150 (MQ=255)                                                                                                                                               cccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTg    >  1:713449/1‑149 (MQ=255)                                                                                                                                               cccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGAc  >  2:220323/1‑151 (MQ=255)                                                                                                                                               cccgGTTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGAc  <  1:773835/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TTGCAATGGAATGACAGCGGGTATGTTAAACAACCCCATCCGGCATGGAGCCAGGTGGACGTTAAATATAAACCTGAAGATTAAACATGACTGAATCTTTTGCTCAACTCTTTGAAGAGTCCTTAAAAGAAATCGAAACCCGCCCGGGTTCTATCGTTCGTGGCGTTGTTGTTGCTATCGACAAAGACGTAGTACTGGTTGACGCTGGTCTGAAATCTGAGTCCGCCATCCCGGCTGAGCAGTTCAAAAACGCCCAGGGCGAGCTGGAAATCCAGGTAGGTGACGAAGTTGAC  >  NC_000913/961909‑962201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑