Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,128,293	IS5 (+) +4 bp	coding (100‑103/318 nt)	metJ ←	transcriptional repressor, S‑adenosylmethionine‑binding

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2290113	NA (NA)	103 (1.420)	75/298	0.0	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 4128296	0 (0.000)					coding (100/318 nt)	metJ	transcriptional repressor, S‑adenosylmethionine‑binding
*	?	NC_000913	3652036 =	NA (NA)	44 (0.600)	38/298	1.9	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	4128293 =	0 (0.000)					coding (103/318 nt)	metJ	transcriptional repressor, S‑adenosylmethionine‑binding

CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3652165‑3652036 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCAC  >  NC_000913/4128293‑4128438                                                                                                                                                                                                                                                                                      CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGG                                                                                                                               >  1:417561/1‑151    AAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAA                                                                                                                             >  1:989983/1‑150      ATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACC                                                                                                                          <  2:556616/151‑1         GCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTA                                                                                                                       <  1:75302/151‑1          CGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTA                                                                                                                       >  1:860400/1‑150           GAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAAT                                                                                                                     <  2:555295/151‑1              GAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGTCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTT                                                                                                                  <  2:925996/151‑1                 ACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTT                                                                                                               <  1:238815/151‑1                     TCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACT                                                                                                           >  2:205441/1‑151                      CTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCCCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGACACCGTAATCTTTTTGACTT                                                                                                          >  2:654048/1‑151                        AAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTG                                                                                                         <  1:340361/150‑1                                  ATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCT                                                                                              <  2:498889/151‑1                                   TTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTT                                                                                             >  2:814659/1‑151                                   TTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTT                                                                                             >  2:347955/1‑151                                      AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCC                                                                                          >  2:798132/1‑151                                         GCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTG                                                                                       >  2:1071030/1‑151                                           ATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCT                                                                                     <  2:501100/151‑1                                               ACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAG                                                                                  <  1:58699/150‑1                                                   GAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTAT                                                                             >  1:138492/1‑151                                                   GAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTAT                                                                             >  2:1132079/1‑151                                                         AAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTG                                                                       <  2:892977/151‑1                                                         AAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTG                                                                       >  2:23199/1‑151                                                             CGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATA                                                                    <  1:245930/150‑1                                                                              AATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCA                                                  <  1:344833/151‑1                                                                                   AATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCAT                                             >  1:689237/1‑151                                                                                   AATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCAT                                             <  2:826563/151‑1                                                                                         TGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAT                                        >  2:1072214/1‑150                                                                                         TGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAT                                        >  2:389689/1‑150                                                                                           AGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCTCCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATAC                                      <  1:233102/150‑1                                                                                              CAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGACTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTA                                   >  1:166740/1‑150                                                                                                 CCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCC                               >  1:168038/1‑151                                                                                                      AAGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTC                          >  2:772398/1‑151                                                                                                       AGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCG                         >  1:490951/1‑151                                                                                                       AGAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCG                         >  1:454439/1‑151                                                                                                        GAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGT                        >  2:298616/1‑151                                                                                                        GAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGT                        <  2:465365/151‑1                                                                                                        GAATTTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGT                        <  1:592478/151‑1                                                                                                            TTCCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAAT                    >  2:711886/1‑151                                                                                                              cctCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTACTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAA                  <  1:425117/148‑1                                                                                                              CCCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAA                  <  1:811205/151‑1                                                                                                               CCCGCTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAA                 <  2:904172/151‑1                                                                                                                   CTTATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTG              >  1:966139/1‑150                                                                                                                     TATTCGCACCTTCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGA             <  2:872595/149‑1                                                                                                                                TCCTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCAC  <  1:376270/149‑1                                                                                                                                                                                                                                                                                      CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3652165‑3652036 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCAC  >  NC_000913/4128293‑4128438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.