Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	650,458	0	A	G	45.3%	74.2 / 10.8	54	T11T (ACT→ACC)	citE	citrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (6/23);  new base G (24/0);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 7.75e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCG  >  NC_000913/650313‑650607                                                                                                                                                  |                                                                                                                                                        aaaCCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGttt                                                                                                                                                     <  1:489472/150‑1 (MQ=255)  aaCCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTa                                                                                                                                                   <  2:904308/151‑1 (MQ=255)   aCCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTAc                                                                                                                                                  <  2:258572/151‑1 (MQ=255)      aTGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCgtt                                                                                                                                               >  1:33945/1‑151 (MQ=255)        gcggcgGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACgttgt                                                                                                                                             <  2:477000/151‑1 (MQ=255)                  gtgtCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAg                                                                                                                                   <  1:582564/151‑1 (MQ=255)                         tttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGaaa                                                                                                                             <  2:469002/150‑1 (MQ=255)                          ttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTTGTTTTTGTAGCGaa                                                                                                                                    >  2:982616/1‑142 (MQ=255)                                cAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCAGCGGAGCGGGAATC‑Atttg                                                                                                                     >  2:14427/1‑148 (MQ=255)                                  aTGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTTGGGGGTTTTCGGTGGTGCAGCGAAGCGGGAATC‑AATgg                                                                                                                     >  2:22190/1‑148 (MQ=255)                                       aCGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAAtcctc                                                                                                             <  2:240829/151‑1 (MQ=255)                                           aGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTAGGTTGGTGCAGCGAAGCCGAGAGC‑ATTGGCAATACTCCCAt                                                                                                         >  1:655863/1‑151 (MQ=255)                                           aGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTTCAGCGAAGCGGAAAAC‑ATTGGCAATCCTccc                                                                                                           >  1:365490/1‑149 (MQ=255)                                            gTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATg                                                                                                        <  2:846998/151‑1 (MQ=255)                                                     gTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGACGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCg                                                                                               >  2:420190/1‑151 (MQ=255)                                                         aaCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGTTTTTCGTTGTTGCAGCGAAGCGGGAAAC‑ATTTGCAATCCTCCCCTGGCAGAGCCggg                                                                                             >  2:278873/1‑149 (MQ=255)                                                          aCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATa                                                                                          <  2:1013618/151‑1 (MQ=255)                                                            aTCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGAATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTTAATTGTTTTAGCGACGCAGAAATC‑AATGAGAAATCTCCCACGGccagggggggaagcc                                                                                        >  1:909556/1‑137 (MQ=255)                                                                gggCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTg                                                                                    <  1:236701/151‑1 (MQ=255)                                                                    aTCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTTGTTGTTTGAGCGAAGCGGGAAAA‑AATGGCAATCCTCCCCTGGGAGAGCCGGGGGACCGCTgg                                                                                   >  1:437270/1‑148 (MQ=255)                                                                    aTCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGgcgc                                                                                <  2:1020509/151‑1 (MQ=255)                                                                         ccGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGTGCGGTTTACGTTGTTCGAGCGCAGCGGAAACC‑AGTGGCAATCTCCCCCTGGCCGCGCCgggggtcccc                                                                                      >  1:468363/1‑133 (MQ=255)                                                                         ccGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTTTTGCAGCCGAGCGGAAATC‑AATGGCAATACCCCCATGGCCGAGCCCCGGTACCCCTGGCGCGTGcc                                                                           >  1:49480/1‑151 (MQ=255)                                                                          cGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCca                                                                          <  2:565645/151‑1 (MQ=255)                                                                          cGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCca                                                                          <  1:899044/151‑1 (MQ=255)                                                                             gTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGTTTTTTGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAAACCTCCCCTGGCAGAGGCGGGGTACCGCTGGCGCGTGCccgc                                                                        >  2:1127454/1‑150 (MQ=255)                                                                               aGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGGTGTTGCAGCGAAACGGGAATCAATTGGC‑ATCCTCCCCTGGCAGAGGCGGGGTACCGCTGGCGCGGGcc                                                                           >  2:942854/1‑145 (MQ=255)                                                                                gATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTGCAGCGAAGCGGAAAAA‑ATTGGGAATACTCCCATCGCAGAGCCGGGGGACCGCCGGCGGGGGGCAGCA‑ggg                                                                   >  1:920863/1‑151 (MQ=255)                                                                                   gAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCtt                                                                <  1:477025/151‑1 (MQ=255)                                                                                    aaGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTc                                                               <  2:707987/151‑1 (MQ=255)                                                                                    aaGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTTTTGCAGCGAAGCGGGAAAC‑ATTTGCAATCCTCCCCTGGCCGAGCCGGGAGACCGCCGGCGCGGGCCCGCA‑GGGcgt                                                                >  1:477700/1‑148 (MQ=255)                                                                                      ggAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCa                                                             <  1:23586/151‑1 (MQ=255)                                                                                           tGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCCGCGGAGCGGGAAAC‑ATTGGCAATACTCCCCTGGCAGAGCCGGGGGACCGCCGGGGCGGGGCAGCA‑GGGGCTCCCGTc                                                          >  2:194817/1‑149 (MQ=255)                                                                                            gCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTg                                                       <  2:72936/151‑1 (MQ=255)                                                                                                 ccATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGt                                                  <  2:477730/151‑1 (MQ=255)                                                                                                  cATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTa                                                 <  1:698019/151‑1 (MQ=255)                                                                                                   aTCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTTGTTGTTTCAGCGAAGAGGGAAAC‑ATTGGCAATCCTCCCCTCGCCAAGCCGGGAGAACCCCGGCGGGTGCCACCA‑GGGCCTCCACTCg                                                         >  1:171224/1‑142 (MQ=255)                                                                                                   aTCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTCGTTTTTGGAGCGAAGCGGAGAAC‑ATTGGCAACCCTCCCCTCGGCGAGGCGGGGGACCCCTGGCGCGTGCCCGCA‑GGGCCTcccacccggcccgca                                                 >  1:85100/1‑137 (MQ=255)                                                                                                     cgcgGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTaaa                                               <  1:582240/150‑1 (MQ=255)                                                                                                        ggCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTACGTTGTTTCAGCGAAGCGGAAAAC‑ATTGGCAATCCTCCCCTTGCAGAGCCGGGGTACCCCCGGGGCGTGCCCGCC‑GGGCTTTCCGTCGTGCACGTaaaa                                              >  2:610575/1‑148 (MQ=255)                                                                                                             tGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTTTGTTTTTTCAGCGAAACGGAAATC‑AATGGCCCACCTCCCCAGGCAGAGCCGGGGTAACCCCGGCGCGTGGCAGCC‑GGGCGTCCCGTCGTGCACGTAAAATGCCGTcc                                      >  1:418592/1‑151 (MQ=255)                                                                                                               gCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTTTGTTGTTGCCGCGAAGAGGAAAAC‑ATTGGCAATCCTCCCCTGGCAGGGGCGGGGTAACGCCGGCGCGGGCCAGCA‑GGGGCTCTCGTCGTGCGCGTAAAATGGAGTcc                                      >  1:703432/1‑149 (MQ=255)                                                                                                                      gCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTGTAGCGAAGAGGGAAAC‑ACTTGCCACCCTCCCCTGGGAGAGCCGGGGGAACCCGGGCGCGTGGCCGCA‑CGGGCTCCCCCCGTGCCCGTAAAAAGCAGTCCCGTgcggc                              >  1:1080259/1‑148 (MQ=255)                                                                                                                        acaaacaaCATGCTGCGGCGGGGGGGGGTTTTTTGTTTTTTCAGCGAAAAGGAAAAC‑AATGGCGATCCTCCCCTGGCAGAGCCGGGGTACCCCTGGGGCGGGCCCGCAGGGGCTTCC‑GGCGCGCACGTGAAAAACAGTCCCGGGCGCCctt                           >  1:74279/1‑151 (MQ=255)                                                                                                                        acaaacaaCATGCTGCGGCGGGGGGGGGGTTTTTGTTTTTGGCGCGAAACGGGAATC‑AATTGCAATCCCCCCCTGGGAGAGGCGGGGTACCGCCGGGGCGTGCCCGCC‑GGGCTTCCAGTCGTGCACGGAAAAAGCCGTCCCGTGCGCCttt                           >  1:749632/1‑151 (MQ=255)                                                                                                                           aacaacATGCTGCGGCGGGTGCGAGTTTTTCGTTTTTTCAGCGAAAAGGGAATC‑ATTGGCAATCCTCCCCTGGCCGAGGCGGGGCACAGCCGGCGCGTGCCCGCG‑GGGCTTCCCGTCGTGCACGTAAAAAACAGGCCCCCGCGCCTTTg                          >  1:838192/1‑149 (MQ=255)                                                                                                                             caacaTGCTGCGGCGGGTGCGGGTTTTTCGTTTTTTGCGCGGAGCGGGAAAC‑ATTGGGAATCCTCCCATGGCAGAGCCGGGGGACCCCTGGCGCGGGCCCGCA‑GGGCGTTCAGTCGTGCCCCTAAAATGCAGTCCAGTGCGCCctt                           >  1:332174/1‑146 (MQ=255)                                                                                                                             caacaTGCTGCGGCGGGTGCGGGGTTTTCGGTTTTTCAGCGGAACGGGAATA‑ATTGGGAAACCCCCCCCTGGAGAGCCGGGGGACCGCCGGCGCGGGGCCGCC‑GGGGCTCCCCTCGTGCGCGGAAAAAGCAAGCCCGTGCGCCctt                           >  1:599334/1‑146 (MQ=255)                                                                                                                               acaTGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATc                     <  2:199417/150‑1 (MQ=255)                                                                                                                                          cggGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTg         >  1:453387/1‑151 (MQ=255)                                                                                                                                             gTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAc      <  2:213490/151‑1 (MQ=255)                                                                                                                                               gCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCTATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAcgc    <  1:143210/151‑1 (MQ=255)                                                                                                                                               gCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAcgc    >  2:1109859/1‑151 (MQ=255)                                                                                                                                                 gAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAcgccg  >  1:919796/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                        AAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATC‑ATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCA‑GGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCG  >  NC_000913/650313‑650607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑