Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913650,4640AT43.6% 73.6 / 25.7 55R9R (CGT→CGAcitEcitrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (7/24);  new base T (24/0);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.11e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCAC  >  NC_000913/650314‑650612
                                                                                                                                                      |                                                                                                                                                      
aaCCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTa                                                                                                                                                        <  2:904308/151‑1 (MQ=255)
 aCCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTAc                                                                                                                                                       <  2:258572/151‑1 (MQ=255)
    aTGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCgtt                                                                                                                                                    >  1:33945/1‑151 (MQ=255)
      gcggcgGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACgttgt                                                                                                                                                  <  2:477000/151‑1 (MQ=255)
                gtgtCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAg                                                                                                                                        <  1:582564/151‑1 (MQ=255)
                       tttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGaaa                                                                                                                                  <  2:469002/150‑1 (MQ=255)
                        ttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTTGTTTTTGTAGCGaa                                                                                                                                         >  2:982616/1‑142 (MQ=255)
                              cAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCAGCGGAGCGGGAATCAtttg                                                                                                                          >  2:14427/1‑148 (MQ=255)
                                aTGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTTGGGGGTTTTCGGTGGTGCAGCGAAGCGGGAATCAATgg                                                                                                                          >  2:22190/1‑148 (MQ=255)
                                     aCGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAAtcctc                                                                                                                  <  2:240829/151‑1 (MQ=255)
                                         aGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTAGGTTGGTGCAGCGAAGCCGAGAGCATTGGCAATACTCCCAt                                                                                                              >  1:655863/1‑151 (MQ=255)
                                         aGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTTCAGCGAAGCGGAAAACATTGGCAATCCTccc                                                                                                                >  1:365490/1‑149 (MQ=255)
                                          gTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATg                                                                                                             <  2:846998/151‑1 (MQ=255)
                                                   gTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGACGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCg                                                                                                    >  2:420190/1‑151 (MQ=255)
                                                       aaCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGTTTTTCGTTGTTGCAGCGAAGCGGGAAACATTTGCAATCCTCCCCTGGCAGAGCCggg                                                                                                  >  2:278873/1‑149 (MQ=255)
                                                        aCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATa                                                                                               <  2:1013618/151‑1 (MQ=255)
                                                          aTCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGAATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTTAATTGTTTTAGCGACGCAGAAATCAATGAGAAATCTCCCACGGccagggggggaagcc                                                                                             >  1:909556/1‑137 (MQ=255)
                                                              gggCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTg                                                                                         <  1:236701/151‑1 (MQ=255)
                                                                  aTCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTTGTTGTTTGAGCGAAGCGGGAAAAAATGGCAATCCTCCCCTGGGAGAGCCGGGGGACCGCTgg                                                                                        >  1:437270/1‑148 (MQ=255)
                                                                  aTCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGgcgc                                                                                     <  2:1020509/151‑1 (MQ=255)
                                                                       ccGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGTGCGGTTTACGTTGTTCGAGCGCAGCGGAAACCAGTGGCAATCTCCCCCTGGCCGCGCCgggggtcccc                                                                                           >  1:468363/1‑133 (MQ=255)
                                                                       ccGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTTTTGCAGCCGAGCGGAAATCAATGGCAATACCCCCATGGCCGAGCCCCGGTACCCCTGGCGCGTGcc                                                                                >  1:49480/1‑151 (MQ=255)
                                                                        cGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCca                                                                               <  1:899044/151‑1 (MQ=255)
                                                                        cGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCca                                                                               <  2:565645/151‑1 (MQ=255)
                                                                           gTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGTTTTTTGTTGTTGCAGCGAAGCGGAAATCATTGGCAAACCTCCCCTGGCAGAGGCGGGGTACCGCTGGCGCGTGCccgc                                                                             >  2:1127454/1‑150 (MQ=255)
                                                                             aGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGGTGTTGCAGCGAAACGGGAATCAATTGGCATCCTCCCCTGGCAGAGGCGGGGTACCGCTGGCGCGGGcc                                                                                >  2:942854/1‑145 (MQ=255)
                                                                              gATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTCGTTGTTGCAGCGAAGCGGAAAAAATTGGGAATACTCCCATCGCAGAGCCGGGGGACCGCCGGCGGGGGGCAGCAggg                                                                        >  1:920863/1‑151 (MQ=255)
                                                                                 gAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCtt                                                                     <  1:477025/151‑1 (MQ=255)
                                                                                  aaGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTc                                                                    <  2:707987/151‑1 (MQ=255)
                                                                                  aaGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTTTTGCAGCGAAGCGGGAAACATTTGCAATCCTCCCCTGGCCGAGCCGGGAGACCGCCGGCGCGGGCCCGCAGGGcgt                                                                     >  1:477700/1‑148 (MQ=255)
                                                                                    ggAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCa                                                                  <  1:23586/151‑1 (MQ=255)
                                                                                         tGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGCGGGGTTTTCGTTGTTGCCGCGGAGCGGGAAACATTGGCAATACTCCCCTGGCAGAGCCGGGGGACCGCCGGGGCGGGGCAGCAGGGGCTCCCGTc                                                               >  2:194817/1‑149 (MQ=255)
                                                                                          gCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTg                                                            <  2:72936/151‑1 (MQ=255)
                                                                                               ccATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGt                                                       <  2:477730/151‑1 (MQ=255)
                                                                                                cATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTa                                                      <  1:698019/151‑1 (MQ=255)
                                                                                                 aTCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTTGTTGTTTCAGCGAAGAGGGAAACATTGGCAATCCTCCCCTCGCCAAGCCGGGAGAACCCCGGCGGGTGCCACCAGGGCCTCCACTCg                                                              >  1:171224/1‑142 (MQ=255)
                                                                                                 aTCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGGGGTTTTCGTTTTTGGAGCGAAGCGGAGAACATTGGCAACCCTCCCCTCGGCGAGGCGGGGGACCCCTGGCGCGTGCCCGCAGGGCCTcccacccggcccgca                                                      >  1:85100/1‑137 (MQ=255)
                                                                                                   cgcgGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTaaa                                                    <  1:582240/150‑1 (MQ=255)
                                                                                                      ggCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTACGTTGTTTCAGCGAAGCGGAAAACATTGGCAATCCTCCCCTTGCAGAGCCGGGGTACCCCCGGGGCGTGCCCGCCGGGCTTTCCGTCGTGCACGTaaaa                                                   >  2:610575/1‑148 (MQ=255)
                                                                                                           tGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTTTGTTTTTTCAGCGAAACGGAAATCAATGGCCCACCTCCCCAGGCAGAGCCGGGGTAACCCCGGCGCGTGGCAGCCGGGCGTCCCGTCGTGCACGTAAAATGCCGTcc                                           >  1:418592/1‑151 (MQ=255)
                                                                                                             gCACCAGGCACAAACAACATGCTGCGGCGGGGGGGGGTTTTTTGTTGTTGCCGCGAAGAGGAAAACATTGGCAATCCTCCCCTGGCAGGGGCGGGGTAACGCCGGCGCGGGCCAGCAGGGGCTCTCGTCGTGCGCGTAAAATGGAGTcc                                           >  1:703432/1‑149 (MQ=255)
                                                                                                                    gCACAAACAACATGCTGCGGCGGGGGGGGGGTTTTTGTTGTTGTAGCGAAGAGGGAAACACTTGCCACCCTCCCCTGGGAGAGCCGGGGGAACCCGGGCGCGTGGCCGCACGGGCTCCCCCCGTGCCCGTAAAAAGCAGTCCCGTgcggc                                   >  1:1080259/1‑148 (MQ=255)
                                                                                                                      acaaacaaCATGCTGCGGCGGGGGGGGGTTTTTTGTTTTTTCAGCGAAAAGGAAAACAATGGCGATCCTCCCCTGGCAGAGCCGGGGTACCCCTGGGGCGGGCCCGCAGGGGCTTCCGGCGCGCACGTGAAAAACAGTCCCGGGCGCCctt                                >  1:74279/1‑151 (MQ=255)
                                                                                                                      acaaacaaCATGCTGCGGCGGGGGGGGGGTTTTTGTTTTTGGCGCGAAACGGGAATCAATTGCAATCCCCCCCTGGGAGAGGCGGGGTACCGCCGGGGCGTGCCCGCCGGGCTTCCAGTCGTGCACGGAAAAAGCCGTCCCGTGCGCCttt                                >  1:749632/1‑151 (MQ=255)
                                                                                                                         aacaacATGCTGCGGCGGGTGCGAGTTTTTCGTTTTTTCAGCGAAAAGGGAATCATTGGCAATCCTCCCCTGGCCGAGGCGGGGCACAGCCGGCGCGTGCCCGCGGGGCTTCCCGTCGTGCACGTAAAAAACAGGCCCCCGCGCCTTTg                               >  1:838192/1‑149 (MQ=255)
                                                                                                                           caacaTGCTGCGGCGGGTGCGGGTTTTTCGTTTTTTGCGCGGAGCGGGAAACATTGGGAATCCTCCCATGGCAGAGCCGGGGGACCCCTGGCGCGGGCCCGCAGGGCGTTCAGTCGTGCCCCTAAAATGCAGTCCAGTGCGCCctt                                >  1:332174/1‑146 (MQ=255)
                                                                                                                           caacaTGCTGCGGCGGGTGCGGGGTTTTCGGTTTTTCAGCGGAACGGGAATAATTGGGAAACCCCCCCCTGGAGAGCCGGGGGACCGCCGGCGCGGGGCCGCCGGGGCTCCCCTCGTGCGCGGAAAAAGCAAGCCCGTGCGCCctt                                >  1:599334/1‑146 (MQ=255)
                                                                                                                             acaTGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATc                          <  2:199417/150‑1 (MQ=255)
                                                                                                                                        cggGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTg              >  1:453387/1‑151 (MQ=255)
                                                                                                                                           gTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAc           <  2:213490/151‑1 (MQ=255)
                                                                                                                                             gCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCTATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAcgc         <  1:143210/151‑1 (MQ=255)
                                                                                                                                             gCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAcgc         >  2:1109859/1‑151 (MQ=255)
                                                                                                                                               gAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTAcgccg       >  1:919796/1‑151 (MQ=255)
                                                                                                                                                    ttACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCa   <  1:506228/150‑1 (MQ=255)
                                                                                                                                                     tACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCAc  <  1:14408/150‑1 (MQ=255)
                                                                                                                                                     tACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCAc  <  1:131581/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGCGGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGGAAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCAGTGCGCCTTTGTCATCGACATTCAGCTGTACGCCGCGCAC  >  NC_000913/650314‑650612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: