Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,506,0200CA42.6% 102.5 / 25.8 54G21C (GGC→TGC) ydcOBenE family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base C (26/5);  new base A (0/23);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 9.29e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGG  >  NC_000913/1505871‑1506162
                                                                                                                                                     |                                                                                                                                              
gagaACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGcc                                                                                                                                                >  2:40076/1‑150 (MQ=255)
    aCAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAAtt                                                                                                                                            >  1:72924/1‑150 (MQ=255)
     cAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTaa                                                                                                                                          <  1:955703/151‑1 (MQ=255)
     cAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTa                                                                                                                                           >  2:126936/1‑150 (MQ=255)
        gTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATAc                                                                                                                                       >  1:444225/1‑151 (MQ=255)
              gATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGcc                                                                                                                                   >  1:339356/1‑149 (MQ=255)
                  ccATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAAc                                                                                                                             <  1:445034/151‑1 (MQ=255)
                   cATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAAcc                                                                                                                            <  1:894545/151‑1 (MQ=255)
                      aaTGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCg                                                                                                                         >  1:294481/1‑151 (MQ=255)
                         gTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGcc                                                                                                                       <  1:352988/150‑1 (MQ=255)
                                  aGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgtgg                                                                                                             >  1:649425/1‑151 (MQ=255)
                                    cGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgtgggt                                                                                                           >  2:399680/1‑151 (MQ=255)
                                        cTGACGCCCATTGCCCGCCCCGGCGCCGTCATCCAGCCAGAGATTTGTGCGGTGGTGGCCCGGCCAAAAATCGCTGCTTGCAATATTATGGCCGCTGAAGTGGGGTAGCAAATTAACACCGCCAGAAACCCCGCCAGTAGCgtgggt                                                                                                           <  1:300814/147‑1 (MQ=255)
                                         tGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggagg                                                                                                      >  1:981940/1‑151 (MQ=255)
                                             gCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAAt                                                                                                   >  1:726347/1‑150 (MQ=255)
                                              cccATTCCCACCCCCGGCGCCGTCACCCAGCCAGAGTTTTGTGCAGGGGGGGCTCCGGCGACAATCGCTGCTTGCAAGTTTATTGCCGCGGAACGGGCGTAGCAAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAAt                                                                                                   <  2:339381/149‑1 (MQ=255)
                                              cccATGGCCAGCCCCAGCGCCGTCATCCAGCCAGAGTTTTGTGCGGGGGGGGCCCGGGCGACAATCGCTGTTGGCCAGATTATGGCCGCGAAACGGGCGGGGAAAATTAATCCCGCCAGAACCCCCGCCAGTAGCGTGGGTgga                                                                                                        <  2:200568/144‑1 (MQ=255)
                                               ccttggCCACCCCCGCCCCCGTCACCCCGCCAGAGTTTTGGGCAGGGGGGCCTCGGGCGAAAATCGCTGCTGGCCAGTTTATGGCCGTGGACCGGGGGTGGAAAATAAATCCCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGaa                                                                                                    <  1:303580/142‑1 (MQ=255)
                                                  ttGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGaaa                                                                                              >  1:1009664/1‑150 (MQ=255)
                                                     ccccccccGGCGCCGTCACCCAGCAAGAGTTTTGTGAAGTGGGGGCCCGGGCAACAATCGCTGCTTCCCAGATTATTGCCGCGGAACTGGGGTAGCAAATTAAACCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGaaa                                                                                              <  2:830178/143‑1 (MQ=255)
                                                     ccACCCCCAGCGCCGTCACCCAGCAAGAGATTTGGGCGGGGGTGGCCCGGGCGACAATCCCTGTTTCCAAGATTATTGCCGTGGAACGGGCGTAGCAAATTAATCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAAc                                                                                            <  2:657554/149‑1 (MQ=255)
                                                         ccccAGCGCCGTCATCCAGCCAGAGTTTTGTGCAGGGGTGGCCCGGGCAACAATCGCTGTTGGCCAGTTTATTGCCGCGAAACGGGCGTAGCAATTTAATCCCGCCAAAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc                                                                                        <  2:923196/149‑1 (MQ=255)
                                                         ccccAGCGCCGCCATCCAGCAAGAGTTTTGGGCGGTGGTGGCCCGGGCGAAAATCGCTGTTTGCCAGTTTATTGCCGCTGACCGGGGGTAGCAAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc                                                                                        <  2:720560/149‑1 (MQ=255)
                                                         ccccAGCCCCGTCACCCACCCAGAGATTTGGGCAGTGGTGGCTCCGGCGACACCCGCTGCTCGCCAGATTATGGCCGCGAAACGGGCTTAGCAAATAAAACCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACg                                                                                       <  1:314935/150‑1 (MQ=255)
                                                         ccccAGCCCCGTCACCCACCAAGATTTTTGGGCAGGGGGGGCCCCGGCAACAACCGCTGTTTCCAAGATTATTCCCGCTGAACGGGCGGAGCAAATAAAACCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGa                                                                                         <  1:759510/148‑1 (MQ=255)
                                                              gccccGTCACCCAGCCAGAGTTTTGTGCAGGGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCAAATAAATCCCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCAt                                                                                    <  2:269323/145‑1 (MQ=255)
                                                                 cggccccccagccagATTTTTGGGAGGGGGGGCCCCCGGCAAAAATCGCTGCTGGCAAGTTTTTGGCGGGTGAAGGGGGGTAGCAAATAAATCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATa                                                                                   <  1:1105385/139‑1 (MQ=255)
                                                                 ccGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCAc                                                                              >  1:752084/1‑151 (MQ=255)
                                                                  cGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCAcc                                                                             >  2:177396/1‑151 (MQ=255)
                                                                   gTCACCCACCAAGAGTTTTGGGCGGGGGTGGCCCCGGCAACACTCGCTGCTGCCCATTTTTTGGCCGATGAGCGGGGGAAGCAAATAAAACCCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCAcc                                                                             <  1:75209/150‑1 (MQ=255)
                                                                     cATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCg                                                                          >  2:144046/1‑151 (MQ=255)
                                                                       cccagccagAATTTTGGGCGGGGGGGGCTCGGGCAAAAACCGCTGCTGGCCAGATTATTGCCGCTGAACGGGCGTAGCAAATTAATCCCGCCAAAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAg                                                                                  <  2:254615/140‑1 (MQ=255)
                                                                             cagAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtat                                                                  >  2:913306/1‑151 (MQ=255)
                                                                              agagTTTTGTGCGGGGGGGCCCCCGGCAAAATCCGCTTCTTGCAAGATTTTTGCCGCGGAACTGGGGGAGCAAATAAATCCCGCAAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtat                                                                  <  1:246624/150‑1 (MQ=255)
                                                                                   tttGTGCAGTGGTGCCTCCGGCAACAACCTCTGCTTCCAAGATTATCGCCGCTAAACTGGCGTACCAAATAAACCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGc                                                               <  2:600720/148‑1 (MQ=255)
                                                                                         cggtggtgGCCCCGGCGACACTCGCTGCTTGCCAGATTATGCCCGCTAACCGGGCGTACCCAATTAATCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCa                                                      <  2:410862/149‑1 (MQ=255)
                                                                                                  cTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCAt                                             >  1:1103494/1‑151 (MQ=255)
                                                                                                     cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTg                                          >  2:759196/1‑151 (MQ=255)
                                                                                                     cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTg                                          >  2:726603/1‑151 (MQ=255)
                                                                                                           cAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGtt                                    >  2:744723/1‑151 (MQ=255)
                                                                                                                gctgctTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAAc                               >  1:286993/1‑151 (MQ=255)
                                                                                                                      tcccAAATTATCCCCGCTAAATGGGCGTGCCAAATAAACCCCCCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACg                              <  1:907049/144‑1 (MQ=255)
                                                                                                                      tGCAAGATTATGGCCGCTGATCGGGGGTAGCAAATTAACCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGca                            <  2:865630/148‑1 (MQ=255)
                                                                                                                       gCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGcaca                          >  1:917827/1‑149 (MQ=255)
                                                                                                                       ccgattttattCCCGCTGAAGTGGGGTAGCAAATAAACCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTtat                                  <  1:781853/135‑1 (MQ=255)
                                                                                                                              tattGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATaa                  >  2:711685/1‑150 (MQ=255)
                                                                                                                                ttGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACt               >  1:21288/1‑151 (MQ=255)
                                                                                                                                   ccGCTGAACTGGCGTAGCAAATTAATCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTc             <  2:172360/150‑1 (MQ=255)
                                                                                                                                    cGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCg           >  1:392709/1‑151 (MQ=255)
                                                                                                                                     gCTGAACGGGCGTAGCAAATTAATCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACt               <  1:1065021/146‑1 (MQ=255)
                                                                                                                                         aaCGGGCGTAGCAAATTAATCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGtttt       <  2:285656/150‑1 (MQ=255)
                                                                                                                                           cTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGcc    >  2:990803/1‑151 (MQ=255)
                                                                                                                                             ggCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCgg  >  1:428495/1‑151 (MQ=255)
                                                                                                                                             ggCGTACCAAATAAACCCCCCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCg   <  1:756869/150‑1 (MQ=255)
                                                                                                                                               cGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCgg  >  1:432536/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
GAGAACAGGTACGCGATACCATAATGTCAGAGTCAGCGTACTGACGCCCATTGCCAGCCCCAGCGCCGTCATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACCGTAGCATTTGTCCGTTATAACGCACAAGTGATAAACTTCCGTTTTGCCGG  >  NC_000913/1505871‑1506162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑