Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,842,914	0	A	G	44.4%	45.3 / 20.8	45	M182V (ATG→GTG)	gdhA	glutamate dehydrogenase, NADP‑specific
Reads supporting (aligned to +/- strand):  ref base A (2/23);  new base G (20/0);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 7.29e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGA  >  NC_000913/1842764‑1843062                                                                                                                                                       |                                                                                                                                                         gATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGga                                                                                                                                                          <  1:550286/151‑1 (MQ=255)      cGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGatgatg                                                                                                                                                     <  2:396862/151‑1 (MQ=255)          ccGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGggtggggaa                                                                                                                                                   >  2:21565/1‑144 (MQ=255)             aaaGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGc                                                                                                                                              <  2:275047/151‑1 (MQ=255)              aaGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGct                                                                                                                                             <  1:1024049/151‑1 (MQ=255)                ggAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGAGGgaaaaaatc                                                                                                                                            >  1:430933/1‑146 (MQ=255)                ggAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGctct                                                                                                                                           <  1:543044/151‑1 (MQ=255)                          aaGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGTGGAAAAAATCTCccacaaaa                                                                                                                                  >  1:674756/1‑148 (MQ=255)                           aGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATAcc                                                                                                                                <  1:384491/151‑1 (MQ=255)                             gTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGGAAAAACTCTccacca                                                                                                                                     >  1:701292/1‑144 (MQ=255)                              tGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGTGGAAAAAGTCTCCCACAAAAcccc                                                                                                                              >  1:149175/1‑148 (MQ=255)                               gAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGATGGAAAAAATCTcccaaaaaaacccc                                                                                                                             >  2:853752/1‑138 (MQ=255)                                       gCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGTGGAAAAACTCTCCCACAAAACcccc                                                                                                                             >  2:610296/1‑142 (MQ=255)                                         gTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGAAGAAAAAAATCTCCCACAAAAACGCCCG‑CGGC‑tt                                                                                                                   >  2:66017/1‑148 (MQ=255)                                           ttttGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CAcc                                                                                                              <  1:459477/150‑1 (MQ=255)                                                  aGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGTGAAAAAAATCTCCCACAAAAACCCCCC‑CGTC‑TT‑TCCCGGGAAggg                                                                                                      >  2:529051/1‑151 (MQ=255)                                                   ggCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAggg                                                                                                      <  2:547510/150‑1 (MQ=255)                                                      gcTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGATGAAAAAACACTCCCCCAATAACCCCCC‑CGCT‑TT‑CCCCCGTAAgg                                                                                                       >  1:1042782/1‑146 (MQ=255)                                                        tgatgaCTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGAAAAAAGTCTCCAACAAAAACCCCTGCCGCC‑TC‑CCCCGGAAGGGG‑CCtt                                                                                                 >  2:659654/1‑150 (MQ=255)                                                        tgatgaCTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGAGGAAAAAATCTCCAACAAAAACCCCCG‑CGGC‑CT‑TCCCGGGAAGGG‑GCtt                                                                                                 >  1:242661/1‑149 (MQ=255)                                                             aCTGAACTGTATCGCCCCCTGGGGGCGGATACCGACTTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTg                                                                                          <  1:523776/151‑1 (MQ=255)                                                              cTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGTGAAAAAAACCTCCCACAAAACCCCCCC‑CGGC‑TT‑TACCCGGAAGGG‑CCtttttttt                                                                                           >  2:565391/1‑144 (MQ=255)                                                               tGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGAAAAAAAGATCCAACAAAACCCCCCG‑CGTC‑TT‑CCCCGGGGAGGG‑CCATTCCttt                                                                                           >  1:126894/1‑148 (MQ=255)                                                                       aTCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCt                                                                                <  1:1016086/151‑1 (MQ=255)                                                                           ccACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTTGTGGAAAAATAATCCCACAACAACGCCCG‑CGGC‑TTCCCCCGGTAGGGG‑CTTTTCTTTTgtgggcgtcctttt                                                                             >  1:251997/1‑138 (MQ=255)                                                                            cACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTc                                                                           <  1:57063/151‑1 (MQ=255)                                                                             aCCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGGGAAAAAGATCTCCAACAATACCCCCTG‑CGGC‑TT‑TAACGGGAAGGGCCCTTTAATTTggtgcggggttttt                                                                             >  1:519871/1‑137 (MQ=255)                                                                                tGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGAAAAAACACTTCCCCCATAACCCCTG‑CGGC‑TT‑CTCCCGGCAGGG‑GGTTTCTTTTTGTGGCGGTCTttttttccc                                                                       >  1:86965/1‑146 (MQ=255)                                                                                           aCCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGTGAAAAAAAACTCCAACAATAACGCCCC‑CCGC‑TT‑CACCCGCGAGGG‑GCCTTCATTTTGCGGCCGGCTTATTTGCCCCGGAGCTa                                                               >  2:171013/1‑148 (MQ=255)                                                                                            ccGACGTGCCGGAAGGTGATATCGGGGTGGGTGGTCGGGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCg                                                            <  1:721556/150‑1 (MQ=255)                                                                                                cGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTa                                                        <  2:539625/150‑1 (MQ=255)                                                                                                 gTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGATGAAAAAAAACTCCAAAAAAAACCCCCG‑CGTCTTT‑CCCCGGAAAGGG‑‑CTTTTATTTTGCGGGGGGCTTTTTTTCCCCGGAGCGACCGGCCAc                                                       >  2:716689/1‑150 (MQ=255)                                                                                                       gCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGATGAAAAAAATCCCCCACAAAAACCCCCG‑CGGC‑TT‑CCCCGGGGAAGG‑GCTTTCCTTTTGCGGCAGGCTTATTTTCCCCGAAGCTAACGGCTACGGTCTgg                                                >  2:1085208/1‑151 (MQ=255)                                                                                                          ggTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGtt                                              <  1:228421/150‑1 (MQ=255)                                                                                                                atCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTca                                       <  2:350233/151‑1 (MQ=255)                                                                                                                   ggggTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTcaca                                     <  1:279255/150‑1 (MQ=255)                                                                                                                   ggggTTGGGGGTCGTGAAGCCGGCTTTATGGCGGGTATGATAAAAAAGCTCTCCAACAAAACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAg                                    <  1:989459/151‑1 (MQ=255)                                                                                                                            ggtCTTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATg                            <  2:1026433/150‑1 (MQ=255)                                                                                                                            ggtCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGc                           <  2:1032857/151‑1 (MQ=255)                                                                                                                             gtCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCt                          <  2:222956/151‑1 (MQ=255)                                                                                                                               cGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTa                         <  2:736601/150‑1 (MQ=255)                                                                                                                               cGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTa                         <  1:6179/150‑1 (MQ=255)                                                                                                                                   aaGTCGGCTTTACGGAGAGGACGATGAAAGACCTCGCAATCAATGCCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑GCTTTCATTTGGCGGCAGTCTTATTCACCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTaaa                       <  1:270377/148‑1 (MQ=255)                                                                                                                                       cGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGc                   <  2:878947/148‑1 (MQ=255)                                                                                                                                        ggCTTTATGGCGGGGGTGATGAAAAAACTCTCCAACAAAAACGCCCG‑CGTC‑TT‑CACCCGTAAGGG‑CCTTTCTTTTTGCGGCGGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGGTTTTTTTCCAGAAACAATGCTAAAAAGCCAc                >  2:959501/1‑150 (MQ=255)                                                                                                                                               tGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATggg        >  1:3681/1‑151 (MQ=255)                                                                                                                                                 gCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGtt      <  2:63799/151‑1 (MQ=255)                                                                                                                                                  cGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGttt     >  1:81792/1‑151 (MQ=255)                                                                                                                                                       atgatgAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGa  >  1:620747/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                         GATTTCGATCCGAAAGGAAAAAGCGAAGGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTG‑CGTC‑TT‑CACCGGTAAGGG‑CCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGA  >  NC_000913/1842764‑1843062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑