| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,555,106 | 0 | C | A | 44.4% | 80.4 / 25.9 | 54 | N177K (AAC→AAA) | uxuR | fructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (10/20); new base A (24/0); total (34/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.75e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTT > NC_000913/4554956‑4555256 | ggCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAc < 1:584362/151‑1 (MQ=255) aCTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑Gaaaaaa > 1:538314/1‑144 (MQ=255) gAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGa < 1:39163/151‑1 (MQ=255) aaGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑GAAAAAAACCAAtgt > 1:390782/1‑147 (MQ=255) agCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCa < 2:875235/151‑1 (MQ=255) agCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGACAGTGGCGGGAAAAACAATcaacaagggtt > 1:625356/1‑139 (MQ=255) gTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGtt < 2:525652/151‑1 (MQ=255) tGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑GGAAAAAATCAATATGGaggcaaagg > 2:625107/1‑143 (MQ=255) ggCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGcaca < 1:299868/149‑1 (MQ=255) ccAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑GAAAAAAACCAAAATGGATccgaggggcacac > 2:1037715/1‑135 (MQ=255) aGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTgg < 1:669191/151‑1 (MQ=255) tGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAAAACAATGTGGGTTCAGTTGCACAGCCCTCTgg > 1:739056/1‑149 (MQ=255) ccGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑GAAAAAATCCAAAGTGGGTCCAGTTGCACAGCCATCTGGGTGa > 1:1035427/1‑149 (MQ=255) ccGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACa < 1:264874/151‑1 (MQ=255) gcagcaGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑GAAAAAATCCAAAGGGGATCCAGTTGCACAGCCATCCGGGTGagaacc > 2:896705/1‑145 (MQ=255) gcagcaGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCt < 1:127338/151‑1 (MQ=255) gcagcaGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAAACCAAAGTGGGTCCCGTTGCACAGCCCTCTGGGTGACAGccc > 1:6728/1‑150 (MQ=255) agcagcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGt < 2:991073/151‑1 (MQ=255) agcGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAAACCAATGTGGGTCCAGTTGCACAGCCATCTGGATGACAGCCTGTa > 2:925355/1‑149 (MQ=255) gAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑GAAAAAATACCCAGTGGATCCAGTTGCACCACCCTCTGGATGAAAGCCCGTATCGc > 1:424857/1‑150 (MQ=255) aaGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCaaa < 1:822263/151‑1 (MQ=255) aGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAag < 1:971294/151‑1 (MQ=255) cGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑GAAAAAAACCCATGTGGATCCCGTTGCACATCCATCTGGATGAGAGCCCGTGTCGTaaa > 2:392672/1‑148 (MQ=255) ggTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGt < 2:192568/151‑1 (MQ=255) gACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAAAACCATGTGGGTCCCGGTGCACAGCCCTCTGGATGACAGCCCGTATCGCAAAGAgggg > 1:953988/1‑150 (MQ=255) tGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAATCCAATGTGGGTCCAGTTGCACCGCCCTCTGGGTGACAGCCCGTATCGCAAAGAGGGGGTggg > 2:493373/1‑151 (MQ=255) tGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGGGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGA‑AAAAAAAACCCATGGGGGATCACGTGAACCGCCCCCTCGGTGACAGCCTGGATCTCGAAAAGGGGtttg > 2:271535/1‑148 (MQ=255) gCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGCGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAAATCTATGTGGATCCCCTTTATCCGCCACCTGGTTGTCACCCTGTTTTCCCAAGAgtgggtgg > 1:1005432/1‑149 (MQ=255) gCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAAACCCATGGGGGTCCCGTTGCACAGCCATCTGGGTGACAGCCCGTATCGCAAAGAGGGGGTggg > 1:144691/1‑150 (MQ=255) tctcGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAATCCAATGTGGGTCCAGTTGCACAGCCATCTGGATGACAACCTGTATCGCCAAGAGGGGTTGGGGGGTCACaa > 2:41164/1‑150 (MQ=255) ctcGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAAACCAAAGTGGGGCCCGTTGGACAGCCACCCGGGTGACAACCCGTATCGCAAAGAGtgggtggggggagacaa > 1:81878/1‑140 (MQ=255) tcGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATTCAGTTTGACAGCCACCTCGGTGACAGCCTGTATCCCAAAGAGGGGGTGGGGGGTCACaaa > 1:899917/1‑149 (MQ=255) cTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAATCCAATATGGAGCCAGTTGCACAGCCATCTTGAGTAGAGCCCGGATCGCAAAGGGTGGGTGGGTGATCCCaaa > 2:197250/1‑146 (MQ=255) ttGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAAACCAAAGTGGGTCCCGTTGCACAGCCCCCCGGGTGGCAGCCCGTATCGCAAAGAGGGGGGGGGTGGTCCCAAACAAATcc > 1:203631/1‑150 (MQ=255) cGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑GAAAAAAAACCATATGGGGCCCGTTTCACAACCCCCTTGGTGACAGCCCGTTTCGCAAAAAGGGGGTTGGGGGGCACaaaaaaaacccc > 2:704240/1‑139 (MQ=255) cGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTcgcc < 2:499862/151‑1 (MQ=255) gAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑GAAAAAATCCCATGTGGGTCCCGTTGCACAGCCCACTGGGTGACAGCCCGGATTGCAAAAAGGGGGTGGGGGATCACAAAAAAAACCTcccc > 1:293993/1‑150 (MQ=255) aaGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTcgccgc < 2:574313/151‑1 (MQ=255) aaGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAAACCAAAGTGGGTTCAGTTGCTCAGCCATCTGGATGACAGACCGTATCGCCAAGAGGGGTTGGGGGGTCACAAACAGATCCTc > 2:472750/1‑146 (MQ=255) aTAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCaa < 1:1072909/151‑1 (MQ=255) tAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAAACCAATGGGGATTCCGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAAAGTGGTTGGGGGATCACAAACAGAACCTCCCCGCGTTAATCaa > 1:1027990/1‑150 (MQ=255) ggtggAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCg < 1:779472/150‑1 (MQ=255) gTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCCATTGGACAGCCCTCTGGGTGACAGGCTGTTTTGCAAAGAGGGGGTGGGCGGACACAAAAAGACCCCCCCCCCGTTTATTAAAAAAAATGCCCgggagg > 1:493522/1‑141 (MQ=255) tCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAg < 1:345547/149‑1 (MQ=255) tCCGTCAGTCCTGGCAGTGGCGGG‑AAAAAAAACCAATGTGGGTCCAGTTGCCCAGCCCTCTGGGTGACAGCCTGTATCGCAAAAAGGGGGTGGGCGATCACAAACAGAACCTCGCCCCCGTAATCAAAAAAAATGCCCGAGCGGCGaa > 1:333957/1‑148 (MQ=255) ccGTCAGTCCTGGCAGTGGCGGG‑AAAACAAACCAATGTGGGTCCAGTTGCACAGCCCTCTTGGTGACAGCCCGTATCGCAAAGAGGGGGTGGGCGATCACCAACAGATCCTCGCCGCGTTTATCAAAAAAAAAGCCCCAGCGGCGGAACTg > 2:820946/1‑151 (MQ=255) ccGTCAGTCCTGGCAGTGGCGGG‑AAAAAAAACCAAAGTGGGTCCAGTTGCCCAGCCATCTTGATGAAAGCCCGTATCGCAAAGAGGGGGTGGGGGGTCCCAAAAAAAACCCCGCCGCGGTAATCAAAAAAAAAGCCCGGGCGGGGGAGCTg > 1:1091556/1‑151 (MQ=255) cGTCAGTCCTGGCAGTGGCGGG‑AAAAAAATCCAATGTGGGTCCAGTTGCACCGCCATCTGGGTGACAGCCTGTATCGCAAAGAGTGGGTTGGCGATCACAAACAGATCCTCGCCGCGGTAATCAAAAAAAATTCCCGAGCGGCGAAGCTgg > 2:112606/1‑151 (MQ=255) cGTCAGTCCTGGCAGTGGCGGG‑AAAAAAATCCAATATGGATCCAGTTGCCCAGCCATCTGGGTGGCAACCCGGATCGCAAAAAGAGGGTGGGCGGACACAAACAAATCCCCGCCGCGTTAATTAAAAAAAAAGCCCCAGCGGGGAAACTgg > 2:266898/1‑151 (MQ=255) gTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGc < 2:664391/151‑1 (MQ=255) ggCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCt > 1:161996/1‑151 (MQ=255) aaaCAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAAc < 2:294016/151‑1 (MQ=255) aaaCAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAAc < 2:1069463/151‑1 (MQ=255) aaCAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACg > 2:438876/1‑151 (MQ=255) aaCAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACg < 2:1049122/151‑1 (MQ=255) aCAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGt > 2:781449/1‑151 (MQ=255) cAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGtt > 2:237491/1‑151 (MQ=255) | GGCATTGCAACTGGAAGAGCGTGAACTGGCTTCCAGTGCGCCGGGCAGCAGCGAAAGCGGTGACATGCAGTTCCATCTCGCTATTGCCGAAGCAACGCATAACAGCATGCTGGTGGAGCTGTTCCGTCAGTCCTGGCAGTGGCGGG‑AAAACAATCCAATGTGGATCCAGTTGCACAGCCATCTGGATGACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTT > NC_000913/4554956‑4555256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |