Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,862,664 | 0 | C | G | 40.9% | 12.7 / 24.9 | 44 | pseudogene (940/1617 nt) | glvC | pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component |
Reads supporting (aligned to +/- strand): ref base C (14/12); new base G (4/14); total (18/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.08e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-01 |
GCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGTTGCGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCG > NC_000913/3862515‑3862809 | gCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGTTGCg < 1:505499/151‑1 (MQ=255) ccATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TTAAAGGTTgggg > 2:579138/1‑151 (MQ=255) ccATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGTTGCggg < 1:704818/151‑1 (MQ=255) gTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGTTGCGGG‑AATc < 1:643397/151‑1 (MQ=255) tGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGGTGCggggaac > 1:907707/1‑145 (MQ=255) cataccaAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATTAACAGTAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGTTGCGGG‑AATC‑AGCAAGccc < 2:917933/148‑1 (MQ=255) aaaGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑GT‑AAAGTTGCGGG‑GATC‑AGCAAAcccccc > 2:96250/1‑148 (MQ=255) aCATTACGGTCGACATGGAGGCCGCCAGCCCGGCGTGTACCGAAAACAGCAACGGTAAAATGAACAGGAAGGAAAATT‑CCGGCGGTTCGG‑TAATCCCCACCAGCAGGGCGG‑TT‑AAGGTGGCGGA‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCg < 1:817419/151‑1 (MQ=255) cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGGTTT‑ATGGTTGCGGG‑AACA‑ACAAAGCCCCCCC‑CTTTT‑CCCCAtttt > 2:580412/1‑150 (MQ=255) tGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGGTGGGGG‑AATC‑CGCAAACCCCCCACCTTTTCCCCCATTTT‑CGGTGCgg > 2:985850/1‑149 (MQ=255) gAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGGTGCGGG‑GATC‑AGCCAGCCCCCCC‑CTTTTACCCCGTTTTTCGGGGCgg > 2:341911/1‑148 (MQ=255) gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑GT‑AAGGGTGCGGG‑GATC‑AAGAAAACCGCCC‑CCTTTTTCCCCTTTTTCGGGGCgggcgtgga > 2:189694/1‑142 (MQ=255) cACGGCGTGTCCCGAAAACAGCAACGGAGAAATGAAAAGGAAGTTAAATC‑CCGGCGGTTCGG‑TAATCCCCACCAGCAGGGGGG‑TT‑AAGTTGGCGGA‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGt < 1:258138/146‑1 (MQ=255) cACGGCGTGCACCGCAAACACCAACGGGAAAAAGAACAGAAAGGTAATTC‑CCAGCGGTTCGA‑AAATCCCCCCCACCGGGGCGT‑TT‑AGGGTGGGGGA‑AATA‑AGCAACCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACAt < 2:1011179/150‑1 (MQ=255) cGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TTAAAGGTTTGGGG‑AA‑A‑AGCAAGACCACCC‑CTTTTACCCCCTTTTTCGGGTTGGCAGTGaa > 1:960286/1‑142 (MQ=255) ggCGTGTCCCGCAAACAGCAACGGTAAAATGAACAGGAAGGTAAATT‑CCGGCGGTTGGG‑TAATCCCCACCAGCATGGCGT‑TA‑AAGGTGGGGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATc < 1:847722/148‑1 (MQ=255) cGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGATTGGGGG‑CATC‑AGTCAACCCCCCA‑CTTTTTCCCGATTTTCCGGTGAGGGAGTGAAgcatatagc > 2:807086/1‑140 (MQ=255) cGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAAGTTGCGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGGAGTACATCGCTAAAGaaa > 1:430362/1‑149 (MQ=255) aaaCAGC‑ACGGGTAAAGGAAAAAGAAAGGAAATTCCCGGGGGTGGGG‑TATTCCCCACAAGCATGGGGG‑TT‑AGGGTGGGGGA‑AATC‑AGCAACCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAAt < 2:398031/147‑1 (MQ=255) aaaCACCAACGGTAAAATGAACAGAAAGATAAATC‑CCAGCGTTTCGG‑TAATTCCCCCCAGC‑TGGGCG‑TT‑TAGGTTGGGGGAAACC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGa < 1:494494/144‑1 (MQ=255) gtaaaagaaaCAGGAAGGTAAATA‑CATGCGGTGCGG‑TAACCCCCCCCGGC‑TGGGTG‑TT‑AAGGGTGGGGGAAATC‑AACAGGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCaa < 1:1007800/142‑1 (MQ=255) aaaTGAACAGGAAGGTAAATC‑CCGGCGGTTGGG‑TATTCCCCCCCAGCAGGGCGG‑TA‑AGGGTGGGGGA‑AATG‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCaaa < 1:532008/148‑1 (MQ=255) gAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGGTGGGGG‑GAAC‑AGCCAGGCCGCCA‑CTTTTAACCGCTTTTCCGGTGCGGGAGTGAAGTGAATAGCTAAAGAAAATCCCCCAGCGCCAAAGAttttt > 1:569745/1‑151 (MQ=255) aaggggaGGAAAAAT‑CCGGGGGTTTGGATAATCCCCACCAGCTTGCCGT‑TT‑AGGGTTGCGGG‑AACC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGAttttt < 2:75236/144‑1 (MQ=255) aCAGGAAGGAAAATC‑CCAGGGTTTCGG‑TAATCCCCCCCAGCAGGGCGG‑TT‑AGGGTGGGGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGa < 1:317374/151‑1 (MQ=255) ggAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGTTGCGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCCGTGAAGTACATCGCTAAAGAAATTCCCACGGCACCAAAGATTTTTTAGtt > 1:682175/1‑151 (MQ=255) gAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGGTGCGGG‑AATC‑AGCAAACCCCCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGGGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGtt > 1:102364/1‑150 (MQ=255) aaGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑AT‑AAGGTTTCGGG‑GAAC‑AGCAAGCCCGCCA‑CTTTTACCCGCTTTTCCGGTGGGGGAGTGGAGTACATCGCCAAAAAAATGCCCACGGCGCCCAAGATTTTTGAGtt > 2:34911/1‑149 (MQ=255) aaGGTAAATC‑CCGGGGGTTGGG‑TAATTCCCCCCAGCATGGCGT‑TT‑AAGGTTGCGGA‑AATC‑ACCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTa < 1:299302/150‑1 (MQ=255) aaaTT‑CCGGCGTTTCGG‑TAATCCCCCCCAGCATGGC‑G‑TT‑AAGGGTGGGGGAAATC‑AGAAACCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTg < 1:846909/150‑1 (MQ=255) aaaTT‑CCGGCGGTGCGG‑TAATCCCCCCCAGCGTGGGGG‑TT‑AAGG‑TGGGGG‑AATCAAGCAGCCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGc < 2:281800/151‑1 (MQ=255) aaaTC‑CCGGCGGTTTGG‑AAATCCCCACCAGCAGGGGGG‑TT‑AGGGTGGGGGA‑AATC‑AAAAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTg < 2:559978/150‑1 (MQ=255) aaTT‑CCAGCGGTTCGA‑TAACCCCCACCAGAGGGGCGT‑TT‑AGGGTGGGGGA‑AATC‑AGAAGCCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCa < 2:967037/151‑1 (MQ=255) cAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGGTGCGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGGGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAggg > 1:339097/1‑149 (MQ=255) ggttggtaaaccccccccAGCAGGGTGG‑TT‑AAGGTGGCGGA‑AACC‑AGAACGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAggg < 2:179668/133‑1 (MQ=255) tCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGGTGCGGG‑AATC‑AACAAACCCGCCC‑CTTTTACCCGATTTTTCGGGGCGGCAGTGAAGTACATCGCTAAAAAAAATCCCACGGCACCAAAGATTTTTTAGTTACCGTGCCGGGCaaaaaccc > 2:767222/1‑147 (MQ=255) tAATTCCCACCAGCATGGCGG‑TT‑AAGGTTGCGGG‑GATC‑AGCAAGCCCGCCC‑CTTTTTCCCGATTTTTCGGTGCGGCAGGGAAGTACATCGCTAAAGAAAATCCCACGGCACCAAAGATTTTTTAGTTACCGTGCAGGGCAAAACCGCCtt > 1:743825/1‑150 (MQ=255) cccACCAGCGTGGGGG‑TT‑AAGGTGGGGGA‑AAGC‑AGAAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCt < 2:519566/144‑1 (MQ=255) tGGCGG‑TT‑AAGGTTGCGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGGGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGAttt > 1:692847/1‑151 (MQ=255) ggCGG‑TT‑AGGGTGGGGGG‑AATC‑AGAAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGAtt < 1:432012/149‑1 (MQ=255) gCGG‑TT‑AAGGTGGCGGA‑AACC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCa < 1:826020/151‑1 (MQ=255) tagggTGGCGGG‑AACC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCgg < 2:566566/147‑1 (MQ=255) aaGGTGGCGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCgg < 1:34954/149‑1 (MQ=255) ggTTGGGGA‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCg < 1:677228/151‑1 (MQ=255) gTTGCGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCg > 1:475004/1‑150 (MQ=255) | GCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATT‑CCAGCGGTTCGG‑TAATTCCCACCAGCATGGCGG‑TT‑AAGGTTGCGGG‑AATC‑AGCAAGCCCGCCA‑CTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCG > NC_000913/3862515‑3862809 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |