Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	46.2%	97.2 / 14.0	66	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (5/30);  new base G (30/0);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.94e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354141‑354435                                                                                                                                                     |                                                                                                                                                    ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTg                                                                                                                                                    <  1:185518/149‑1 (MQ=255)      aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGaa                                                                                                                                                  >  1:780198/1‑146 (MQ=255)        ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATgc                                                                                                                                            <  1:397064/150‑1 (MQ=255)          gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGGAATTAACGTTGCCGGGGATCGGCTGGGGACGCGCGAGATTGAAGGGAGTATCTCCAGCCGTCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCt                                                                                                                                         <  2:69246/151‑1 (MQ=255)               gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAg                                                                                                                                    <  2:146438/151‑1 (MQ=255)                 tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTGGGGGGGAAGAAGGGGTGGAAggg                                                                                                                                  >  2:947588/1‑151 (MQ=255)                  gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAggg                                                                                                                                  <  1:737928/150‑1 (MQ=255)                  gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGc                                                                                                                                 <  2:486259/151‑1 (MQ=255)                   aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCa                                                                                                                                <  2:342518/151‑1 (MQ=255)                   aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCa                                                                                                                                <  1:463842/151‑1 (MQ=255)                          tcgcTTTATTCTCGGGCGCGCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggg                                                                                                                          <  2:935059/147‑2 (MQ=255)                          tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGGAAGGGCAggtggcg                                                                                                                         >  1:272184/1‑151 (MQ=255)                          tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGTTGGGGTGAAAAATGCGCCGGAAAGGGAggggg                                                                                                                           >  2:895588/1‑149 (MQ=255)                            aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggt                                                                                                                       <  1:714049/151‑1 (MQ=255)                            aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAAAAGCGCTGCAAAGGCCggtggcgg                                                                                                                        >  1:1008249/1‑150 (MQ=255)                              tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGCGGC‑GGGGGGTGGGGGGGGAGATGGGCTGAAAGGGGAgggggcggggg                                                                                                                     >  2:101730/1‑151 (MQ=255)                                tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAGAT‑CGGCGAAAGGGCCggagggggagggg                                                                                                                   >  1:223811/1‑149 (MQ=255)                                  ttCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGtt                                                                                                                 >  1:451150/1‑151 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                              <  1:516536/150‑1 (MQ=255)                                           gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGTTTGTCATTcc                                                                                                        >  1:953666/1‑151 (MQ=255)                                              cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTcc                                                                                                        >  1:769899/1‑148 (MQ=255)                                              cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAAGCGGTGAGAGGGGAGGGGGGGGGGGGGGTTGTctct                                                                                                          >  2:521651/1‑143 (MQ=255)                                                    aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAagagag                                                                                               <  1:469872/151‑1 (MQ=255)                                                    aTGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGGGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTTCGACagaggg                                                                                               >  2:158820/1‑149 (MQ=255)                                                               gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                    <  1:82254/151‑1 (MQ=255)                                                                ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGGGGTGGGGGTTGTCCTTCCGGAAGAGAGCGACAGGCTgg                                                                                    >  1:672024/1‑150 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                 <  1:709699/151‑1 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCGCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                                                                                 <  2:798122/151‑1 (MQ=255)                                                                    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAAGGCAGGGGGGGGGGGGGGTTGTCATTCCCAAAAAGAGCGACAGTCTGGAAggt                                                                               >  1:971043/1‑149 (MQ=255)                                                                     gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGAGGCGCTGAAAGGGCCGGGGGGGGGGGGGTTTGTCATTTCGGAAGAGAGCGGCAGGCTgg                                                                                    >  1:620893/1‑145 (MQ=255)                                                                     gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTTGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGgaga                                                                                >  1:991201/1‑149 (MQ=255)                                                                       gCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGt                                                                            <  1:827571/151‑1 (MQ=255)                                                                         aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTCCGAAAGAGAGCGACAGGCTTGGAGATCGTg                                                                           >  1:792155/1‑150 (MQ=255)                                                                         aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGGGGGTTGGGTGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGTTTGTTATTTCGGAAAAGAGGGACAGGCTggga                                                                                  >  1:213934/1‑141 (MQ=255)                                                                           cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAt                                                                         <  1:733832/150‑1 (MQ=255)                                                                            ggatGGAGACGAGCAAGACTGAAGAGAGTGTCTAGTGCCGTCCGAGCGTTGCCAGAGTAGC‑AGTGGTTGGGGTGAAGGATGCGCTGACAGGGCAGGTGGCGGTGGCGTCTGTGATTTCGAAAGAGATCGACAGGCGGGGAGGTCGTGAtgg                                                                       <  2:186290/148‑2 (MQ=255)                                                                            ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAAAAGCGCTGAAAAGGAAGGGGGGGGTGGCGTTTGTCATTCCGAAAAAGAGCGAAAGACTGGAAGa                                                                                >  2:982768/1‑142 (MQ=255)                                                                             gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                      <  1:765519/151‑1 (MQ=255)                                                                              cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                     >  1:969907/1‑151 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGTGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGTGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTTATGTgg                                                                     <  2:59011/149‑1 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgc                                                                    <  2:826873/150‑1 (MQ=255)                                                                                   gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCGGGGGGCGGTGGCGTTTGTTCTTCCCAAAGAGAGCGCCAGTCTGGGAGATTGTGATGTGgcg                                                                   >  1:995870/1‑148 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAAATGCGCTGAAAGGGCAGGGGGCGGGGGGGTTTGTCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                      >  2:964749/1‑138 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGGAAGAAGCGCTGAAAGGGCAGGGGGCGGTGGGGGTTGTCATTCCGGAAGAGAGCGACAGTCTGGGAGATCGGGGTGGGGGGCACTCGcca                                                          >  2:787940/1‑146 (MQ=255)                                                                                             aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGGAAGATGCGCTGAAAGGGCAGGTGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGGGGCGCACTCGCaa                                                          >  2:209867/1‑147 (MQ=255)                                                                                             aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                         >  1:110377/1‑148 (MQ=255)                                                                                             aTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGGTGCGCCGAAAGGGCAGGCGGCGGGGGCGTTTGTTATTCCGAAAGAGAGCGACAGTCTTGGAGATCGTGGTGGGGGGCACtgccaa                                                          >  2:291726/1‑142 (MQ=255)                                                                                              ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGAGGCGCCGAAAGGGCCGGGGGGGGGGGGGGTTGTTATTTCGAAAGGGAGCGCCAGGCTGGGCGATCGTGGTGTGGCGCCCTCGcca                                                          >  2:983773/1‑144 (MQ=255)                                                                                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGAGAAACGCGCTGAAACGGCAGGTGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAATTCGGAAGATCGTGATGGGGCGCCCTCCCaa                                                          >  2:642905/1‑141 (MQ=255)                                                                                                    agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGGGTTTGTCATTTCGAAAGAGAGCGACAAGCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                 >  1:362942/1‑149 (MQ=255)                                                                                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                              <  2:746093/151‑1 (MQ=255)                                                                                                         aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGAAGCGCTTTAAGGGCAGGAGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGACTGGAAGATCGTGATGTGGGGCACTCGCAAGAGAAGGCGATTAAgg                                          >  1:101999/1‑151 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCt                                      <  1:354598/151‑1 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTAGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCt                                      <  1:458516/151‑1 (MQ=255)                                                                                                                  cATCCGGGCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAAGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTTGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCtggtgg                                 >  1:31683/1‑151 (MQ=255)                                                                                                                   aTCCGGGCGTTGCCGAAGTGGC‑GGTGGTTTGGGGGGAAGATGCGCCTTAAGGGGAGGTGGCGGGGGGGGTTGTCATTCCGGAAGGGGGGGAGAGCGAGGAAAATTGTGGTGTGGCGCCCTCGCAAGAGAAGGCGAGTATGGGGCtggggg                                 >  2:431499/1‑150 (MQ=255)                                                                                                                   aTCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGGGAAAGATGGGCTGAAAAGGCAGGGGGCGGGGGCGGTTGGCATTCCGAAAGAGAGCGACAGTCTGGAAGGTGGGGGGGTGGCGGAGTCGCCAGATGAGGGGGTTATTGCGCtggggg                                 >  2:368318/1‑150 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGC‑GGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGGGGTTGTCCTTCCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGACa                              >  1:750491/1‑149 (MQ=255)                                                                                                                              gCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                      <  1:587313/150‑1 (MQ=255)                                                                                                                                        c‑GGTGGGTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg           <  2:294362/151‑1 (MQ=255)                                                                                                                                           gtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc         >  2:392372/1‑151 (MQ=255)                                                                                                                                             ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc       <  1:278031/151‑1 (MQ=255)                                                                                                                                             ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg        <  1:213537/150‑1 (MQ=255)                                                                                                                                                tGGGGAGAAAGACGCGCTGAAATGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGATGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAGTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg    >  2:371812/1‑151 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg    <  2:997417/150‑1 (MQ=255)                                                                                                                                                  gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc  <  1:112988/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGC‑GGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354141‑354435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑