Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,152	T→G	L268R (CTG→CGG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,152	0	T	G	100.0%	205.1 / NA	53	L268R (CTG→CGG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/26);  total (27/26)

CGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATC  >  NC_000913/4186007‑4186297                                                                                                                                                  |                                                                                                                                                  cGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTAtc                                                                                                                                              <  2:54057/151‑1 (MQ=255)   tATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTAtcgt                                                                                                                                            >  2:179470/1‑151 (MQ=255)   tATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTAtcg                                                                                                                                             >  2:979284/1‑150 (MQ=255)     tCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTAtcgtcg                                                                                                                                          >  1:426371/1‑151 (MQ=255)          ctgctgGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTc                                                                                                                                      >  1:31811/1‑150 (MQ=255)           tgctgGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCTGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCAt                                                                                                                                    >  1:992536/1‑151 (MQ=255)            gctgGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCAtt                                                                                                                                   <  2:207817/151‑1 (MQ=255)            gctgGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCAt                                                                                                                                    <  2:996306/150‑1 (MQ=255)            gctgGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCAt                                                                                                                                    <  1:28947/150‑1 (MQ=255)              tgGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTa                                                                                                                                  >  2:987048/1‑150 (MQ=255)               ggAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTa                                                                                                                                  <  2:88081/149‑1 (MQ=255)                   gCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTa                                                                                                                            <  2:194361/151‑1 (MQ=255)                     gttcgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaac                                                                                                                          >  1:659382/1‑151 (MQ=255)                        cgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTaaca                                                                                                                         <  1:346775/149‑1 (MQ=255)                               tCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCt                                                                                                                  >  2:1019281/1‑149 (MQ=255)                                   gTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACg                                                                                                            >  1:550587/1‑151 (MQ=255)                                     aaCAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGt                                                                                                           <  1:523628/150‑1 (MQ=255)                                        aaaCCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTctgc                                                                                                       >  2:697676/1‑151 (MQ=255)                                         aaCCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTctgct                                                                                                      >  2:477854/1‑151 (MQ=255)                                           ccAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTgg                                                                                                    >  2:109624/1‑151 (MQ=255)                                               agTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCt                                                                                                >  1:614411/1‑151 (MQ=255)                                                         ccTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCg                                                                                      >  1:896125/1‑151 (MQ=255)                                                               cGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAcatc                                                                                <  1:594291/151‑1 (MQ=255)                                                                  tCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACatcatc                                                                             >  1:312915/1‑151 (MQ=255)                                                                   cTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcg                                                                            >  1:971655/1‑151 (MQ=255)                                                                     gCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCTCGACTTCTGACCTGAACGAGCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAcatcata                                                                             <  2:301407/148‑2 (MQ=255)                                                                     gCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcg                                                                            <  2:193592/149‑1 (MQ=255)                                                                      ccGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgtac                                                                         <  2:942073/151‑1 (MQ=255)                                                                       cGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgtacg                                                                        <  2:169888/151‑1 (MQ=255)                                                                       cGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgtacg                                                                        >  1:829415/1‑151 (MQ=255)                                                                        ggTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATcgtacgt                                                                       <  2:923278/151‑1 (MQ=255)                                                                               ccgccAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGaaa                                                                <  1:592696/151‑1 (MQ=255)                                                                                 gccAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGaaaaa                                                              <  2:394887/151‑1 (MQ=255)                                                                                 gccAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGaaaa                                                               >  1:73161/1‑150 (MQ=255)                                                                                            gTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTAtgctgc                                                    >  2:407934/1‑150 (MQ=255)                                                                                                gCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGaa                                               <  2:449758/151‑1 (MQ=255)                                                                                                  tGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGc                                             >  1:615462/1‑151 (MQ=255)                                                                                                   ggTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGc                                             <  1:473179/150‑1 (MQ=255)                                                                                                       ccGCTGGATTGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTa                                         <  2:597663/150‑1 (MQ=255)                                                                                                         gCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGAc                                      >  1:950243/1‑151 (MQ=255)                                                                                                         gCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGGCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGAc                                      <  2:625635/151‑1 (MQ=255)                                                                                                           tggatggaGGTCGTTTAGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAAACGTCTGAAACGTCTGCTGGATCTGGCTATGCCGAACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGTCGc                                    >  2:566090/1‑151 (MQ=255)                                                                                                           tGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGTAAAACGTATGCTGCAGGAAGCGGTAGACGc                                    >  2:1007741/1‑151 (MQ=255)                                                                                                           tGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGc                                    >  1:300396/1‑151 (MQ=255)                                                                                                              aTGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCct                                 >  2:978991/1‑151 (MQ=255)                                                                                                                 gtggtCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCctgct                              <  2:971528/151‑1 (MQ=255)                                                                                                                   ggtCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCctgctg                             <  1:382664/150‑1 (MQ=255)                                                                                                                   ggtCGTTTCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTgg                            <  2:659296/151‑1 (MQ=255)                                                                                                                          tCGCGACTTCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACgg                     >  1:652260/1‑151 (MQ=255)                                                                                                                                 ttCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGgtcgtc                <  2:647748/149‑1 (MQ=255)                                                                                                                                  tCTGACCTGAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGtcgtcg               <  2:1001304/149‑1 (MQ=255)                                                                                                                                          gAACGATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCg     <  2:894658/151‑1 (MQ=255)                                                                                                                                              gATCGGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATc  >  2:268255/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTACGTAACGAAAAACGTATGCTGCAGGAAGCGGTAGACGCCCTGCTGGATAACGGTCGTCGCGGTCGTGCGATC  >  NC_000913/4186007‑4186297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑