Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,485	(G)5→4	coding (116/930 nt)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,481	0	G	.	100.0%	214.5 / NA	45	coding (112/930 nt)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/22);  total (23/22)

TCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAG  >  NC_000913/1806333‑1806619                                                                                                                                                     |                                                                                                                                           tCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCgg                                                                                                                                           <  2:118991/150‑1 (MQ=255)         aGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGggcggcggc                                                                                                                                 <  1:653552/151‑1 (MQ=255)          gACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGggcggcggc                                                                                                                                 >  1:579145/1‑150 (MQ=255)              gAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCa                                                                                                                             >  2:573850/1‑150 (MQ=255)               aaaTCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAAc                                                                                                                           <  1:600363/151‑1 (MQ=255)               aaaTCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAAc                                                                                                                           >  1:570260/1‑151 (MQ=255)                    aGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGc                                                                                                                      >  1:112680/1‑151 (MQ=255)                           aggaggAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGcc                                                                                                               >  1:699052/1‑151 (MQ=255)                            ggaggaAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGcc                                                                                                               >  2:190680/1‑150 (MQ=255)                              aggaAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCAtt                                                                                                            <  1:223966/151‑1 (MQ=255)                                     gatgGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCAt                                                                                                      <  1:738037/150‑1 (MQ=255)                                      atgGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCt                                                                                                    <  2:468792/151‑1 (MQ=255)                                      acggcaCGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATc                                                                                                     >  1:21042/6‑150 (MQ=255)                                        ggTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTg                                                                                                  <  2:176964/151‑1 (MQ=255)                                         gTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTgg                                                                                                 <  1:462719/151‑1 (MQ=255)                                         gTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTgg                                                                                                 >  2:652988/1‑151 (MQ=255)                                           aCGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTgg                                                                                                 >  2:780122/1‑149 (MQ=255)                                           aCGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTgg                                                                                                 >  1:18536/1‑149 (MQ=255)                                                        ttGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGc                                                                                  >  2:742988/1‑151 (MQ=255)                                                        ttGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGc                                                                                  >  1:331666/1‑151 (MQ=255)                                                           acacTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGAt                                                                               <  1:91609/151‑1 (MQ=255)                                                                tGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTcc                                                                          >  1:442678/1‑151 (MQ=255)                                                                  cgcCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCcg                                                                        >  1:229891/1‑151 (MQ=255)                                                                        ctctcGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCggg                                                                   >  2:659624/1‑150 (MQ=255)                                                                            cGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTg                                                                 <  1:551335/148‑1 (MQ=255)                                                                            cGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGgcg                                                              >  2:745496/1‑151 (MQ=255)                                                                             gATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCAGCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGTTgt                                                                <  1:622152/148‑2 (MQ=255)                                                                                  cgcAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCg                                                        <  1:137025/151‑1 (MQ=255)                                                                                               cctcAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTggg                                           <  1:109453/148‑2 (MQ=255)                                                                                                cGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGtt                                          >  1:524242/1‑151 (MQ=255)                                                                                                   aaaTTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTc                                        <  2:673823/150‑1 (MQ=255)                                                                                                       ttATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGt                                   >  1:437509/1‑151 (MQ=255)                                                                                                             cGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCgg                             >  1:773531/1‑151 (MQ=255)                                                                                                                aGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATg                          <  2:692512/151‑1 (MQ=255)                                                                                                                  gAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATg                          >  1:61540/1‑149 (MQ=255)                                                                                                                  gAAAACTGCGCTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGaa                        <  2:524233/151‑1 (MQ=255)                                                                                                                         gcgcTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTcc                 >  2:548271/1‑151 (MQ=255)                                                                                                                           gcTGTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTcccc               >  1:68158/1‑151 (MQ=255)                                                                                                                              gTACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTc            <  1:698077/151‑1 (MQ=255)                                                                                                                               tACCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt             >  2:641477/1‑149 (MQ=255)                                                                                                                                aCCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCg           <  2:850537/150‑1 (MQ=255)                                                                                                                                aCCGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCg           <  1:699165/150‑1 (MQ=255)                                                                                                                                 ccGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCt         <  2:259972/151‑1 (MQ=255)                                                                                                                                 ccGCACCGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCt         <  2:259363/151‑1 (MQ=255)                                                                                                                                      ccGGTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGt    <  1:843658/151‑1 (MQ=255)                                                                                                                                        ggTGTTCGAACCC‑GGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAg  <  1:796090/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                           TCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAG  >  NC_000913/1806333‑1806619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑