Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,994,461 (T)8→7 pseudogene (632/633 nt) ygeK ← pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,994,4540T.100.0% 280.0 / NA 58intergenic (+13/+6)pbl/ygeKpseudogene, peptidoglycan‑binding enzyme family/pseudogene, response regulator family, part of T3SS PAI ETT2 remnant; putative 2‑component transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (27/31);  total (27/31)

GGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTT  >  NC_000913/2994309‑2994600
                                                                                                                                                 |                                                                                                                                                  
ggCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCtttttt                                                                                                                                               <  2:826478/151‑1 (MQ=255)
ggCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCttttt                                                                                                                                                <  2:362143/150‑1 (MQ=255)
 gCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCttttttt                                                                                                                                              <  2:644253/151‑1 (MQ=255)
   aaTGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCtttttttat                                                                                                                                            <  1:830380/151‑3 (MQ=255)
   aaTGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAACCTAAAGCCTCtttttttat                                                                                                                                            >  2:290542/1‑149 (MQ=255)
    aTGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCtttttttat                                                                                                                                            >  2:708679/1‑148 (MQ=255)
       ccTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAg                                                                                                                                       <  1:657287/151‑1 (MQ=255)
        cTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGt                                                                                                                                      >  2:247452/1‑151 (MQ=255)
          ggggACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGt                                                                                                                                      <  2:552412/149‑1 (MQ=255)
          ggggACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTg                                                                                                                                     <  2:210827/150‑1 (MQ=255)
           gggACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCa                                                                                                                                   <  1:215299/151‑1 (MQ=255)
            ggACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCaa                                                                                                                                  <  1:531863/151‑1 (MQ=255)
            ggACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCa                                                                                                                                   >  2:481810/1‑150 (MQ=255)
             gACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCaaa                                                                                                                                 <  1:184961/151‑1 (MQ=255)
                            ttttGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTaaa                                                                                                                   >  1:528229/1‑150 (MQ=255)
                               tGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTg                                                                                                               >  1:504042/1‑151 (MQ=255)
                                  aaTGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGtt                                                                                                             <  1:146855/150‑1 (MQ=255)
                                         aaCCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCaa                                                                                                     <  2:678053/151‑1 (MQ=255)
                                          aCCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAAc                                                                                                    <  2:588145/151‑1 (MQ=255)
                                          aCCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAAc                                                                                                    >  1:162682/1‑151 (MQ=255)
                                            cAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTc                                                                                                  <  1:329818/151‑1 (MQ=255)
                                             aaaaaaaGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCg                                                                                                 <  1:393867/151‑1 (MQ=255)
                                                      gaCAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACtg                                                                                        >  1:174502/1‑151 (MQ=255)
                                                       aCAGCAATATACCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACtg                                                                                        >  1:99955/1‑150 (MQ=255)
                                                        cAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACtgtg                                                                                      <  2:95876/151‑1 (MQ=255)
                                                         aGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGa                                                                                     <  1:553342/151‑1 (MQ=255)
                                                         aGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGa                                                                                     <  1:131230/151‑1 (MQ=255)
                                                               atGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCt                                                                               >  2:289917/1‑151 (MQ=255)
                                                                 gCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTa                                                                              >  2:701887/1‑150 (MQ=255)
                                                                    ccAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAAttt                                                                          >  1:352479/1‑151 (MQ=255)
                                                                       aaaTATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATttctt                                                                       >  2:243456/1‑151 (MQ=255)
                                                                           atatatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCAt                                                                    <  1:84730/150‑1 (MQ=255)
                                                                             atatTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGat                                                                 >  1:274028/1‑151 (MQ=255)
                                                                                    atataCCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAAc                                                                                                    <  1:28397/109‑1 (MQ=255)
                                                                                    atataCCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAAc                                                                                                    >  2:28419/1‑109 (MQ=255)
                                                                                    atataCCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGt                                                          <  1:771872/151‑1 (MQ=255)
                                                                                         ccAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATg                                                     >  2:38167/1‑151 (MQ=255)
                                                                                         ccAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATg                                                     >  2:107670/1‑151 (MQ=255)
                                                                                          cAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGa                                                    <  1:241711/151‑1 (MQ=255)
                                                                                            ggCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGt                                                  >  1:652677/1‑151 (MQ=255)
                                                                                                tAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGttt                                                <  2:837529/149‑1 (MQ=255)
                                                                                                tAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTc                                               <  1:493880/150‑1 (MQ=255)
                                                                                                    gAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTg                                          >  2:855865/1‑151 (MQ=255)
                                                                                                     aaCTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGt                                         <  2:300859/151‑1 (MQ=255)
                                                                                                     aaCTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGt                                         >  2:413947/1‑151 (MQ=255)
                                                                                                           aCAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCt                                   <  1:358157/151‑1 (MQ=255)
                                                                                                              aTCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCt                                >  2:250528/1‑151 (MQ=255)
                                                                                                                 gTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTaa                             <  2:438597/151‑1 (MQ=255)
                                                                                                                     ggCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGc                         <  2:643360/151‑1 (MQ=255)
                                                                                                                     ggCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGc                         >  2:355260/1‑151 (MQ=255)
                                                                                                                       cTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCa                        >  1:152573/1‑150 (MQ=255)
                                                                                                                        ttCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCa                        >  1:660623/1‑149 (MQ=255)
                                                                                                                         tCAGCAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCaa                       <  2:265903/149‑1 (MQ=255)
                                                                                                                             cAAAATGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAAtt                 >  1:102039/1‑151 (MQ=255)
                                                                                                                               aaaTGAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTa                <  1:538460/150‑1 (MQ=255)
                                                                                                                                  tGAGCCTCAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCg             >  1:174233/1‑150 (MQ=255)
                                                                                                                                   gAGCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGAt           >  2:728289/1‑151 (MQ=255)
                                                                                                                                     gCCTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGAttt         <  2:569914/151‑1 (MQ=255)
                                                                                                                                      ccTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTc        >  1:738700/1‑151 (MQ=255)
                                                                                                                                      ccTAAAGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTc        <  1:711840/151‑1 (MQ=255)
                                                                                                                                         aaaGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCt       <  2:197731/149‑1 (MQ=255)
                                                                                                                                          aaGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCtttg    <  1:185978/151‑1 (MQ=255)
                                                                                                                                          aaGCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCtttg    <  1:642836/151‑1 (MQ=255)
                                                                                                                                            gCCTCTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCtttgtt  >  2:339082/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGCAATGCCTGGGGACTTATAATGCGGGTTTTGCAATGGATAACCAAAAAAAGAGACAGCAATATGCCCCAAAATATATATTGTATATACCAGGCTTAATGAACTAGACAATCGTAAGGCTTCAGCAAAATGAGCCTAAAGCCTCTTTTTTTTATATAGTGCAAACACCCATACGTAAAGCTGTTTTGATCAACTCGATACCACTGTGAACATCTAATTTCTTCATGATATTGAGTCTATGAGTTTCGACTGTTTTCCTGCTTAACTGCAAGAAATTAGCGATTTCTTTGTT  >  NC_000913/2994309‑2994600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: