| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 483,728 | Δ1 bp | coding (676/3150 nt) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 483,726 | 0 | T | . | 86.2% | 95.3 / 6.4 | 31 | coding (678/3150 nt) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); major base . (20/5); minor base C (0/4); total (21/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.31e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTG > NC_000913/483582‑483865 | gTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACcg > 1:453130/1‑151 (MQ=255) caccacCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACcggcgg < 2:104067/150‑1 (MQ=255) caccacCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCggcggc > 1:343411/1‑151 (MQ=255) cAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTaccac < 2:784229/151‑1 (MQ=255) cAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGc > 2:603196/1‑151 (MQ=255) gcgACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑Cccgc > 1:765018/1‑151 (MQ=255) gcgACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTAGAC‑TTCACCGGCGGCGAACCACCGAGCTGA‑Cccgc > 1:313869/1‑151 (MQ=255) cgACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑Cccgcc > 1:287391/1‑151 (MQ=255) gACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑Ctcgc > 1:539458/1‑149 (MQ=255) cgGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTgcgc > 1:585859/1‑151 (MQ=255) ccATCCTGTTTCTCTTGCAGCAGGATTTTGCCAACCTTTTCAGTAGAGGTCGGGGTCGTCGGAGAAAAAATAGGGGCTTTATGGTGTTGG‑CATCCCCCGGCGGCTCCCCCCGGAGCGGC‑CCCGCCGCAACCTGGGCGTTCTGCGCtt < 1:234002/147‑1 (MQ=255) aTCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATgg > 1:182263/1‑151 (MQ=255) tCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGc > 1:700536/1‑151 (MQ=255) ccTGTTCCATTTACAGCAGTTTTTTGCCAACCTCTTCAGTAGGGGTAGGCGGCGTCTGAAAAAAAATAGGGGCTTAAAGTTGTGGGCC‑TTCCCCGGCGGCGTCCCCCCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCg < 2:438177/151‑1 (MQ=255) cACTTTCAGCGGGATTTTCCCGATCTTTTCAGTAGAGGTCAGCCGCGTCGGAGAAAAAATGGGGGCTTTAAGCTGTT‑GCCCTCCCCCGGCGGCTTCCCCCCGGGCTGC‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAAt < 2:181375/149‑1 (MQ=255) aCTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGAc > 2:828845/1‑151 (MQ=255) cagcagGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCa > 2:594287/1‑150 (MQ=255) ggagatttttGCCGACCTCTTCAGGAGAGGCCAGCCGCGTCTGAAAAAAAAGAGGGGCATTAGGCTGTTGGCC‑TCCCCCGGCGCCGTCCCCCGGGGCTCA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCaa < 2:267491/143‑1 (MQ=255) tttGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGtt > 2:8617/1‑150 (MQ=255) tGCCAACCTCTGCAGTAGAGGTCAGCCGCGTCTGAAAAATAAGGGGGGCGTAAAGTTG‑TGGCCCTCCCCGGGGGGCCTCCCCCGGAGCTGC‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAg < 1:109432/150‑1 (MQ=255) aCTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAAt > 2:461147/1‑151 (MQ=255) tctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAAttt > 2:712672/1‑151 (MQ=255) ctTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTg > 1:631798/1‑151 (MQ=255) ggggtcgaccgcgTTGGAGCAAAAATAGGGGCGTTAGGCTGTT‑GCCCTTCCCCGGCGGCGTCCCACCGAGTTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCt < 2:74134/141‑1 (MQ=255) aGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCgg > 1:420059/1‑151 (MQ=255) gcgTCGGAACAAAAATGGGGTCTTAAAGCTGTT‑GCCCTCCCCCGGCGCCTTCCCA‑CGAACCGACCCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCggg < 2:264716/148‑1 (MQ=255) aGCAATAATAGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATcc > 1:61356/1‑148 (MQ=255) aataGAGGCGTTAAGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGc > 2:395438/1‑150 (MQ=255) aagagagGCGTTAGGTGGTTGGCC‑TCCCCGGGCGGCGCCCCACCGGGCTGC‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCa < 1:595804/148‑1 (MQ=255) gCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGt > 2:443159/1‑151 (MQ=255) aaGCTGTTGGCC‑TTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACtgtg > 2:92964/1‑151 (MQ=255) | GTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCCGAACTCTTCAGTAGAGGTCAGACGCGTCTGAGCAATAATAGAGGCGTTAAGCTGTTGGCCTTTCACCGGCGGCGTACCACCGAGCTGA‑CCCGCCGCAACCTGGGCGTTCTGCGCTTTGATGGCGGTAATGACATCAACCGGCGTTAGCTGGAATTTGTTCAGCTCATTCGGGTTCATCCAGATACGCATCGCGTACTGTG > NC_000913/483582‑483865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |