Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,727,494	(G)6→7	coding (836/1098 nt)	nemA →	chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,727,488	1	.	G	100.0%	139.0 / NA	40	A277G (GCG→GGG)	nemA	chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (20/20);  total (20/20)

GCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGT  >  NC_000913/1727340‑1727633                                                                                                                                                      |                                                                                                                                                  gCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gg                                                                                                                                                 >  2:729803/1‑151 (MQ=255) gCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑g                                                                                                                                                  <  1:287551/150‑1 (MQ=255)       cGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑ggggggg                                                                                                                                            <  1:229800/150‑2 (MQ=255)         cATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtga                                                                                                                                         <  2:270418/151‑5 (MQ=255)         cATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtg                                                                                                                                          >  1:733578/1‑147 (MQ=255)         cATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑gggggggtg                                                                                                                                          >  1:468318/1‑147 (MQ=255)           ttGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATAGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAAc                                                                                                                                        >  2:538125/1‑151 (MQ=255)                   cgcgTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACt                                                                                                                                >  2:278099/1‑151 (MQ=255)                        ttCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATg                                                                                                                            <  1:729426/150‑1 (MQ=255)                               aTCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTTTCTGATTGAACAACTGGGTAAACGAGGCATAGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGtt                                                                                                                        >  1:759423/1‑147 (MQ=255)                                               acacAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGccc                                                                                                    >  2:242706/1‑151 (MQ=255)                                                cacaGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCg                                                                                                   >  1:740062/1‑151 (MQ=255)                                                   aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGttt                                                                                                <  2:406559/151‑1 (MQ=255)                                                   aGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGttt                                                                                                >  1:663498/1‑151 (MQ=255)                                                       aaCGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCAc                                                                                            >  1:311055/1‑151 (MQ=255)                                                               gAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTcc                                                                                       <  1:326309/148‑1 (MQ=255)                                                                    aagaagCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCg                                                                               <  2:9692/151‑1 (MQ=255)                                                                          ccGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAg                                                                         <  2:805079/151‑1 (MQ=255)                                                                               gCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGc                                                                     >  2:750309/1‑150 (MQ=255)                                                                                  cTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATac                                                                 >  1:327220/1‑151 (MQ=255)                                                                                   tGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATac                                                                 >  2:197260/1‑150 (MQ=255)                                                                                          gATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTa                                                           <  2:537314/149‑1 (MQ=255)                                                                                          gATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAg                                                          <  2:662964/150‑1 (MQ=255)                                                                                              gAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGaaaaa                                                     <  1:570852/151‑1 (MQ=255)                                                                                                acaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGATCCAGATTGGGCGGGGGGGTGAACAGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGc                                                   <  2:26351/151‑1 (MQ=255)                                                                                                acaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGc                                                   >  1:309247/1‑151 (MQ=255)                                                                                                acaacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGc                                                   >  2:396121/1‑151 (MQ=255)                                                                                                  aacTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTg                                                 >  2:233816/1‑151 (MQ=255)                                                                                                   acTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTg                                                 >  2:115371/1‑150 (MQ=255)                                                                                                      gggTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAAc                                             <  1:68025/151‑1 (MQ=255)                                                                                                         tAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCt                                          >  2:213359/1‑151 (MQ=255)                                                                                                          aaaCGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCt                                          <  2:663540/150‑1 (MQ=255)                                                                                                          aaaCGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTg                                         >  1:317140/1‑151 (MQ=255)                                                                                                          aaaCGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTg                                         <  2:341874/151‑1 (MQ=255)                                                                                                              gcgGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCg                                     <  1:521025/151‑1 (MQ=255)                                                                                                                     tGCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAgg                              >  1:477206/1‑151 (MQ=255)                                                                                                                      gCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAggg                             <  1:781298/151‑1 (MQ=255)                                                                                                                      gCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAgg                              <  2:213662/150‑1 (MQ=255)                                                                                                                      gCTTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAgg                              <  1:267283/150‑1 (MQ=255)                                                                                                                              gCATATGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGa                     <  2:688514/151‑1 (MQ=255)                                                                                                                                atatGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATg                   <  1:213542/151‑1 (MQ=255)                                                                                                                                  atGTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGc                  <  1:762255/150‑1 (MQ=255)                                                                                                                                    gTCAGAACCAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCgg                <  2:109434/150‑1 (MQ=255)                                                                                                                                            cAGATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTg       >  1:283704/1‑151 (MQ=255)                                                                                                                                              gATTGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGt     >  1:701116/1‑151 (MQ=255)                                                                                                                                                 tGGGCGGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGt  >  1:339827/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                  GCCGATCGCATTGGCATTCGCGTTTCACCAATCGGTACTTTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGCTTATCTGCATATGTCAGAACCAGATTGGGC‑GGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCGGTGGCATTTGGTCGT  >  NC_000913/1727340‑1727633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑