Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,812,657	(T)7→8	coding (476/804 nt)	ydjO ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,650	1	.	T	98.2%	199.8 / ‑3.0	54	coding (483/804 nt)	ydjO	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (31/22);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

CATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑TTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATACAGAAGTGACAT  >  NC_000913/1812502‑1812795                                                                                                                                                      |                                                                                                                                                  cATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑t                                                                                                                                                  <  2:144717/150‑1 (MQ=255)  aTACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑ttt                                                                                                                                                <  2:579718/151‑1 (MQ=255)   tACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑tttt                                                                                                                                               >  1:657634/1‑151 (MQ=255)   tACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑tttt                                                                                                                                               >  1:649908/1‑151 (MQ=255)    aCCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑ttttt                                                                                                                                              <  2:740612/151‑1 (MQ=255)    aCCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑ttttt                                                                                                                                              >  1:407290/1‑151 (MQ=255)       cGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑tttttttt                                                                                                                                           <  1:139968/151‑2 (MQ=255)        gagaCGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑ttttttttg                                                                                                                                          <  2:441663/151‑3 (MQ=255)          gaCGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑ttttttttga                                                                                                                                         >  2:817182/1‑147 (MQ=255)             gTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCg                                                                                                                                      >  1:157410/1‑151 (MQ=255)               tttCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCgc                                                                                                                                     <  2:827274/150‑1 (MQ=255)                  cTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCgcagc                                                                                                                                  >  1:259609/1‑150 (MQ=255)                             aTCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTcc                                                                                                                       <  2:557711/150‑1 (MQ=255)                             aTCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTc                                                                                                                        <  2:433353/149‑1 (MQ=255)                                tGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGAc                                                                                                                   >  1:628807/1‑151 (MQ=255)                                 ggAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCg                                                                                                                     <  1:421065/148‑1 (MQ=255)                                 ggAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGa                                                                                                                    <  1:387288/149‑1 (MQ=255)                                 ggAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGAc                                                                                                                   >  1:233605/1‑150 (MQ=255)                                    aTACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAgg                                                                                                                >  2:578737/1‑150 (MQ=255)                                        tCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGt                                                                                                            >  2:512503/1‑150 (MQ=255)                                         cATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGta                                                                                                           <  1:94988/150‑1 (MQ=255)                                                tCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAAttt                                                                                                   >  2:584307/1‑151 (MQ=255)                                                   tCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAAtt                                                                                                    <  1:713953/147‑1 (MQ=255)                                                   tCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTg                                                                                                 <  1:330732/150‑1 (MQ=255)                                                    cAAGCACTCCTCTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGATGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAAtt                                                                                                    <  1:434350/146‑1 (MQ=255)                                                    cAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGtt                                                                                               >  2:299398/1‑151 (MQ=255)                                                    cAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGtt                                                                                               <  2:145354/151‑1 (MQ=255)                                                            ccacTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGtat                                                                                       >  1:294227/1‑151 (MQ=255)                                                                        cccGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTaa                                                                            >  2:620792/1‑150 (MQ=255)                                                                        cccGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTaa                                                                            <  1:775013/150‑1 (MQ=255)                                                                          cGGAAGATTTAGTGGAGTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATACTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAAt                                                                          <  1:298935/150‑1 (MQ=255)                                                                               gATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGa                                                                     >  2:31558/1‑150 (MQ=255)                                                                                 tttAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAAt                                                                   >  2:58047/1‑150 (MQ=255)                                                                                   tAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATaa                                                                 <  1:111340/150‑1 (MQ=255)                                                                                   tAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATaa                                                                 <  2:505250/150‑1 (MQ=255)                                                                                     gTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATaacc                                                               >  1:594683/1‑150 (MQ=255)                                                                                      tGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATaacca                                                              >  2:791258/1‑150 (MQ=255)                                                                                       ggATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATaaccaac                                                            >  1:197980/1‑151 (MQ=255)                                                                                         aTTAAGAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCt                                                          <  1:781515/151‑1 (MQ=255)                                                                                         aTTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCt                                                          >  1:340414/1‑151 (MQ=255)                                                                                          ttAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTg                                                         >  2:824740/1‑151 (MQ=255)                                                                                            aaaaaGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGtt                                                       <  2:503400/151‑1 (MQ=255)                                                                                                       tGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTg                                             <  1:728169/150‑1 (MQ=255)                                                                                                        gATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGa                                            <  2:2852/150‑1 (MQ=255)                                                                                                        gATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑TTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGAt                                           <  2:139537/150‑1 (MQ=255)                                                                                                              aaGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAAt                                      <  2:46124/150‑1 (MQ=255)                                                                                                              aaGCATTGATATTATCGAAGGAAAATTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAGATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCaaa                                       >  1:628429/1‑149 (MQ=255)                                                                                                               aGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTg                                    >  2:516468/1‑151 (MQ=255)                                                                                                                gCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTgg                                   >  2:437967/1‑151 (MQ=255)                                                                                                                   ttGATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTc                                 >  1:337144/1‑150 (MQ=255)                                                                                                                     gATATTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCAtt                              >  2:166009/1‑151 (MQ=255)                                                                                                                      atatTATCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGAAAATAATTTTGTTCATAGTATTTAGTCCTTAAATAGTGATTAACAACCCTGTTTTTTACACTGATCAAATTGGCCAttt                             >  2:176701/1‑151 (MQ=255)                                                                                                                          tatCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTc                         >  1:305108/1‑151 (MQ=255)                                                                                                                           atCGAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCt                        >  2:684321/1‑151 (MQ=255)                                                                                                                              gAAGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTggg                     <  1:31536/151‑1 (MQ=255)                                                                                                                                aGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTg                       >  1:487123/1‑147 (MQ=255)                                                                                                                                aGGAAACTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGATTTTTATACTGATCAACTTGGTCATTTTTTCTGGGCg                   >  2:101108/1‑151 (MQ=255)                                                                                                                                   aaaCTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGaa                 >  2:560445/1‑150 (MQ=255)                                                                                                                                    aaCTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAAt               >  2:830232/1‑151 (MQ=255)                                                                                                                                    aaCTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAAt               <  2:163314/151‑1 (MQ=255)                                                                                                                                      cTTTAGGAGACTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATAc             >  1:516478/1‑151 (MQ=255)                                                                                                                                            gagaCTCTCATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATACAGAAGt       <  1:684278/151‑1 (MQ=255)                                                                                                                                                 tctcATTTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATACAGAAGTGACAt  >  2:251161/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                  CATACCCGAGACGTTTTCTAAGTGAACTATCTGGAATACTCATAATATCTTCAAGCACACCACTTCCCATTCCCGGAAGATTTAGTGGATTAAAAAGAAGATTGATATTAAGCATTGATATTATCGAAGGAAACTTTAGGAGACTCTCA‑TTTTTTTGAATCGCAGCAAAAATGCTCCCGACAGGATGTATATAATTTTGTTCATAGTATATAGTCCTTAAATAGTGAATAACCAACCTGTTTTTTATACTGATCAAATTGGTCATTTTTTCTGGGCGAAATACAGAAGTGACAT  >  NC_000913/1812502‑1812795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑