| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,967,611 | (C)5→6 | coding (891/1050 nt) | cheB ← | fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,967,606 | 1 | . | C | 81.8% | 125.7 / 22.9 | 44 | coding (896/1050 nt) | cheB | fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA |
| Reads supporting (aligned to +/- strand): ref base . (1/7); new base C (17/19); total (18/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑CCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCC > NC_000913/1967459‑1967749 | cGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑ccc < 2:370272/151‑1 (MQ=255)cGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑ccc > 2:768492/1‑151 (MQ=255) tATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑ccccc > 2:55894/1‑151 (MQ=255) aTCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑cccccc < 2:429871/151‑2 (MQ=255) cGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑ccccccg < 1:509277/150‑3 (MQ=255) ccTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑ccccccgcct > 1:787104/1‑146 (MQ=255) ccTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑ccccccgcct < 2:411791/151‑6 (MQ=255) gTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTg < 1:123778/149‑1 (MQ=255) gTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGAc < 2:678828/151‑1 (MQ=255) ccGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGc > 2:616270/1‑151 (MQ=255) gCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATc > 2:592510/1‑151 (MQ=255) ttGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTc > 1:792087/1‑151 (MQ=255) cATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCcgccgc > 1:713610/1‑151 (MQ=255) aTTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCcgccgcg > 2:44601/1‑151 (MQ=255) gCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCcgt < 2:171512/151‑1 (MQ=255) cTGGCTTCCCTGGCTAAGATCGACCACTTCGCAGCCACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCGCGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCcgccgcgccg < 2:341191/149‑1 (MQ=255) gCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGtt > 1:174029/1‑151 (MQ=255) ttACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑CCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGcc > 2:178996/1‑151 (MQ=255) ccTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCACGCGCGGCATGACGAACACCACGCAACTTGCTTCGTTTTGCGCAAGCGGC‑CAT‑GCCCCCCGCCTGACGCATCGCCATCATTCCCGCCGCGCCGTCGTTGccc > 2:432413/1‑150 (MQ=255) tGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCAt > 1:304907/1‑150 (MQ=255) ggCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATAc > 2:278698/1‑151 (MQ=255) gCTAAGATCGACCACTTCGCAGACACCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATa < 1:634781/149‑1 (MQ=255) caaaGATCGACCACCTCGCAGACACCCCCCATATGGATGGCCACGCGCGCATTGCCGAACACCTCTCACCTTGCTTCTTTTGGCGCTAGGTCCGCTA‑C‑CCCCCGCCGTCCGCACCGCCAAAATTCCCGCCGCGCCGTCGTTGCCCATAcc < 1:768435/148‑1 (MQ=255) aaGATCGACCACTTCGCAGACACCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCg < 2:180870/149‑1 (MQ=255) aGATCGCCCACTTCGCAGACCCCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACCCCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTc < 1:393872/151‑1 (MQ=255) cGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGa < 2:583380/151‑1 (MQ=255) ccACTTCGCAGACCCCACCCATATTGATGGCCTCGCGCGGCATGCCGAACCCCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCa < 2:595053/151‑1 (MQ=255) acacCCCCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCAAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAAc < 1:727284/147‑1 (MQ=255) ccccccccATATTGAGGGCCTCGCGCGGCATGCCGAACCCCACGCAACTTGCTTCGTTTTGCGCAGGGGTC‑CAT‑GCCCCCCGCCTGACGCACCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGc < 1:647783/144‑1 (MQ=255) accaccCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCAtt > 2:25272/1‑151 (MQ=255) ccaccCATATTGATGGCCCCGCGCGGCAGGCCGAACACCACGCACCTTGTTTCGTTTTGCCCAGGGGTC‑CAT‑GCCCCCCGCCTGACGCTTCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTa < 2:44955/151‑1 (MQ=255) cccAAATTGGGGGCCTCGCGCGGCATGCCGAAACCCACGCAACTTCCTTTGTTTTCCGAAAGGGCC‑CAG‑G‑CCCCCCCCGGACGCACCCCCAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGcc < 1:849855/151‑1 (MQ=255) atatTGATGGCCTCGCGCGGCATGCCGAACACCACCCACCTGGCTTCGTTTTGCGCAAGGTTC‑CAT‑GCCCCCCGCCGGACGCACCGCCACCATACCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGccc < 1:262978/150‑1 (MQ=255) ggCCCCGCGCGGCATGCCGAACCCCACCCACCTGGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGttt < 1:32412/151‑1 (MQ=255) ccTCCCGCGCCATGCCGACCCCCCCGCACCTTGCTTCTTTTTGCGAAAGGTTC‑CAT‑GCCCCCCCCCTACCGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGttt < 2:736881/149‑1 (MQ=255) cTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGc > 2:277757/1‑151 (MQ=255) cgGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGAc > 2:411818/1‑148 (MQ=255) ccGACCCCCCCGCAACTTGCTTCTTTTTGCGAAGGGGTC‑CAT‑GCCCCCCGCCTGACCCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaac < 2:563382/151‑1 (MQ=255) aaCCCCCCGCAACTTGCTTCGTTTTGCCCAGGGGTC‑CAT‑GCCCCCCGCCGGCCGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaacaa < 2:590846/150‑1 (MQ=255) aaCACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaacaac > 1:74160/1‑151 (MQ=255) caccccGAACCTTGCTTCGTTTTGCGCAAGGGCC‑CATCC‑CCCCCGCCTGCCGCATCGCCAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAacaaca < 2:743294/150‑1 (MQ=255) ccccGCACCTTGCTTCGTTTTGCGCAGGGGTC‑CAT‑GCCCCCCCCCTGACGCATCGCCACCATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAacac < 2:222079/146‑1 (MQ=255) aaCTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCg > 2:172683/1‑151 (MQ=255) aaCTTCCTTCTTTTTGCGCAAGGCTC‑CATCC‑CCCCCGCCTGTCGCCCCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCACCCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTAc < 2:570043/149‑1 (MQ=255) cTTCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGcc > 1:581671/1‑151 (MQ=255) ttCGTTTTGCGCAAGGGTC‑CATCC‑CCCCCGCCTGACGCATCGCCAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGc < 2:246797/149‑1 (MQ=255) ttCGTTTTGCGCAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCg > 1:658884/1‑151 (MQ=255) ttttGCGAAAGGGTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGAt < 2:792143/149‑1 (MQ=255) cgcAGGGGTC‑CATCC‑CCCCCGCCGGCCGCACCGCCAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCACATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACg < 1:194868/148‑1 (MQ=255) gggTC‑CATCC‑CCCCCCCCTGACGCACCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAAccg < 2:545537/151‑1 (MQ=255) gTC‑CAT‑GCCCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAAccgcc > 1:754001/1‑151 (MQ=255) | CGTATCGCCTGTCCGGCACTAATTTTTGCCAACATTTGCTGGCTTACCTGGCTAAGATCGACCACTTCGCAGACACCACCCATATTGATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTC‑CAT‑G‑CCCCCGCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCC > NC_000913/1967459‑1967749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |