| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,627,887 | (G)5→6 | coding (1052/2244 nt) | yfgF ← | cyclic‑di‑GMP phosphodiesterase, anaerobic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,627,882 | 1 | . | G | 97.9% | 157.8 / ‑4.0 | 47 | coding (1057/2244 nt) | yfgF | cyclic‑di‑GMP phosphodiesterase, anaerobic |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (27/19); total (28/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CCGAAAGCTGATAAACATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑GGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAAGAGA > NC_000913/2627735‑2628025 | ccGAAAGCTGATAAACATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑ggg < 2:55243/151‑1 (MQ=255) gATAAACATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑ggggggcatc < 2:63661/149‑6 (MQ=255) cATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAAcg > 1:261387/1‑151 (MQ=255) aTCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAAcgc > 1:231095/1‑151 (MQ=255) ttCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAAcgcgcg > 1:293768/1‑151 (MQ=255) ttCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAAcgcgcg > 1:53366/1‑151 (MQ=255) cACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGAt < 2:382576/151‑1 (MQ=255) aCCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGAtt > 1:844074/1‑151 (MQ=255) cGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTc > 2:267055/1‑149 (MQ=255) ggTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAgg > 2:407117/1‑151 (MQ=255) aaaGGGTGACAGTCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGTCATAGTTCTTAACCAGCATTTCCATGCAAGGGATGCGTAAATTACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGa > 1:534079/2‑149 (MQ=255) gTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTg > 2:446450/1‑151 (MQ=255) aCAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCa < 2:397254/151‑1 (MQ=255) ccAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGAt > 2:614963/1‑150 (MQ=255) ccAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATg > 1:820739/1‑151 (MQ=255) gaAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAaccacc < 2:844132/151‑1 (MQ=255) gTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGt < 1:723708/151‑1 (MQ=255) gCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACAt > 1:520925/1‑151 (MQ=255) tCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCt > 2:117007/1‑151 (MQ=255) cGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGa < 1:722224/151‑1 (MQ=255) gcagcaTGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAACTAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGa > 2:349581/1‑150 (MQ=255) agcaTGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATg > 1:686102/1‑151 (MQ=255) agcaTGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATg > 2:138386/1‑151 (MQ=255) atgatgCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGt < 2:730999/151‑1 (MQ=255) gatgCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTc < 2:165188/150‑1 (MQ=255) tCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑GGGGGTCATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGctc > 1:508978/1‑151 (MQ=255) ttAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGctctc > 2:487164/1‑151 (MQ=255) tAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGctctct > 1:801695/1‑151 (MQ=255) cAGCATTTCCAGGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACg > 1:359319/1‑151 (MQ=255) gCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACggg < 1:759045/151‑1 (MQ=255) cATGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAg < 1:761217/151‑1 (MQ=255) aTGCCAGGGATGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGt > 1:594778/1‑151 (MQ=255) aTGCGTAAATAACAAGGCGCAGACCAGGGGGG‑CATCACGCACCGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCata < 1:36096/151‑1 (MQ=255) aTGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCata < 2:446068/151‑1 (MQ=255) aTGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCat > 2:206959/1‑150 (MQ=255) tGCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCatat > 1:33104/1‑151 (MQ=255) gCGTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCAtata < 1:2461/151‑1 (MQ=255) gTAAATAACAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAAt < 2:50453/151‑1 (MQ=255) aaCAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCTAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACa < 2:743546/150‑1 (MQ=255) aaCAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGCACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACa > 1:32611/1‑150 (MQ=255) aaCAAAGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCTGTACAGCCATATAATTGACa < 2:262204/150‑1 (MQ=255) aaaGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATa < 1:691136/150‑1 (MQ=255) aaGCGCAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATaa > 1:53938/1‑150 (MQ=255) gcgcAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATaaaa < 2:492909/150‑1 (MQ=255) gcAGACCAGGGGGG‑CATCACTCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAagag < 2:116716/151‑1 (MQ=255) cggACCAGGGGGG‑CATCGCGCAGCGCGCAATTCAGGGCGCGAACATTGGCCAGAGGAACAACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGCCTGCCGCACTCCGACGGGTTGCGAGTGCAGCCATATAATTGACATTAAAaga < 1:832422/147‑1 (MQ=255) cAGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAag > 2:654464/1‑148 (MQ=255) aGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAAgaga > 1:709454/1‑150 (MQ=255) aGACCAGGGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAAgaga > 2:675613/1‑150 (MQ=255) | CCGAAAGCTGATAAACATCTTCACCCGGTTCCAGCAAGGGTGACAGCCAGTGAGAAAGTTTTTGCTTGTATTGAATCCGCAGCATGATGCCATAGTTCTTAACCAGCATTTCCATGCCAGGGATGCGTAAATAACAAAGCGCAGACCA‑GGGGG‑CATCACGCAACGCGCGATTCAGGGCGCGAACATTTGGCAGATGAACCACCGGGTCCACATACGCAAGCCGCTGAATGCGTCTGACTACCGCTCTCTGACGGGTTGCCAGTACAGCCATATAATTGACAATAAAAGAGA > NC_000913/2627735‑2628025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |