Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,049,805	(T)5→6	coding (863/1095 nt)	gcvT ←	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,049,800	1	.	T	100.0%	162.1 / NA	44	G290S (GGC→AGC)	gcvT	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (18/26);  total (18/26)

GTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCG  >  NC_000913/3049658‑3049945                                                                                                                                                |                                                                                                                                                  gTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttttcg                                                                                                                                           <  2:470299/151‑4 (MQ=255) gTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑ttttttcg                                                                                                                                           >  2:64424/1‑148 (MQ=255) gTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑tttttt                                                                                                                                             >  2:522709/1‑148 (MQ=255)            aCCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCCGGGAGAAAGTACCGCTGGAGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCAcc                                                                                                                                   >  1:102960/1‑149 (MQ=255)             ccTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGa                                                                                                                                <  1:489984/151‑1 (MQ=255)                tCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGacc                                                                                                                              <  1:488686/150‑1 (MQ=255)                 ccGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGTCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGaccaa                                                                                                                            <  1:305300/151‑1 (MQ=255)                       cgcgcgCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGt                                                                                                                       <  2:698842/150‑1 (MQ=255)                         cgcgcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGtttt                                                                                                                    <  1:244280/151‑1 (MQ=255)                           cgcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTc                                                                                                                  <  1:90177/151‑1 (MQ=255)                           cgcCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTc                                                                                                                  >  1:735410/1‑151 (MQ=255)                              cAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGt                                                                                                               <  1:43784/151‑1 (MQ=255)                               aGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTa                                                                                                              <  2:761020/151‑1 (MQ=255)                                       gCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCt                                                                                                       <  2:735453/150‑1 (MQ=255)                                       gCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTc                                                                                                      <  2:382878/151‑1 (MQ=255)                                         tGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCAc                                                                                                    >  1:450547/1‑151 (MQ=255)                                          gTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCAc                                                                                                    <  1:141149/150‑1 (MQ=255)                                          gTAACCAAGAGTCGGGGAGAAAGTGCCGCTGGTGATAATGCCTTCATGCTGGTTCCCCTGCGCATCGGTAAAGCGTGCCGGCAGTTCATTACGCCGCACGCCTTTTTTCGGTCATCACCTGACCAACCTCTTTTTCTGTACCATGCTcg                                                                                                     <  1:782728/149‑2 (MQ=255)                                           tAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTGCCATGCTCACGc                                                                                                  >  1:767051/1‑151 (MQ=255)                                                         ggAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGc                                                                                    <  1:758806/151‑1 (MQ=255)                                                              aaGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCAc                                                                               <  2:79517/151‑1 (MQ=255)                                                                       tGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATa                                                                       >  2:746900/1‑150 (MQ=255)                                                                       tGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATa                                                                       >  2:163258/1‑150 (MQ=255)                                                                           gATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTc                                                                  >  1:176988/1‑151 (MQ=255)                                                                            aTAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGTGCTTCACGACCGATAAAGTCa                                                                 <  1:728282/151‑1 (MQ=255)                                                                             tAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCAc                                                                >  2:271226/1‑151 (MQ=255)                                                                                 gCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATc                                                            >  2:266997/1‑151 (MQ=255)                                                                                         gCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGtt                                                    >  2:172398/1‑151 (MQ=255)                                                                                               tGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAgg                                              >  2:545412/1‑151 (MQ=255)                                                                                                gCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCGGCAATTCCGGGGCTTTACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGc                                             >  1:465695/1‑151 (MQ=255)                                                                                                  ccTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCATCCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGc                                             <  1:247823/149‑1 (MQ=255)                                                                                                           cGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAg                                   <  2:783323/150‑1 (MQ=255)                                                                                                           cGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAg                                   <  1:215557/150‑1 (MQ=255)                                                                                                             gTAAAGCGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGccc                                <  2:280087/151‑1 (MQ=255)                                                                                                                   cGTACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGtt                           <  2:644804/150‑1 (MQ=255)                                                                                                                     tACCGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggc                        >  1:557521/1‑151 (MQ=255)                                                                                                                        cGGCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTggcggc                     >  1:857727/1‑151 (MQ=255)                                                                                                                         ggCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGTCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCt                    >  2:744411/1‑151 (MQ=255)                                                                                                                         ggCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCt                    <  2:608628/151‑1 (MQ=255)                                                                                                                          gCAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTa                   <  1:679759/151‑1 (MQ=255)                                                                                                                           cAGTTCATTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCCATGGTCCAGCCCATGTTGGCGGCTa                   <  1:128554/150‑1 (MQ=255)                                                                                                                                 aTTACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAgg               >  1:408727/1‑148 (MQ=255)                                                                                                                                  ttACGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGgag             >  1:102708/1‑149 (MQ=255)                                                                                                                                   ttcGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGgagag           <  1:442272/148‑1 (MQ=255)                                                                                                                                    aCGCAGCACGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGAt         <  1:302795/151‑1 (MQ=255)                                                                                                                                          caCGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGttt    <  2:646477/150‑1 (MQ=255)                                                                                                                                           aCGCCTTTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCg  >  1:149594/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                  GTTTCGCCAATACCTTCCGGCACGCGCGCCAGCGCAATGCTGTAACCCAGCGTCGGGGAGAAAGTACCGCTGGTGATAATGCCTTCATGCTGGTTGCCCTGCGCATCGGTAAAGCGTACCGGCAGTTCATTACGCAGCACGCC‑TTTTTCGGTCATCACCAGACCAACCAGTTTTTCTGTACCATGCTCACGCTGCACTTCCAGGGCTTCACGACCGATAAAGTCACGATCTGCCGGTTCCCAGGCGATGGTCCAGCCCATGTTGGCGGCTAAAGGAGAGATGGTTTCG  >  NC_000913/3049658‑3049945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑