| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,511,541 | (G)5→6 | coding (874/957 nt) | fecD ← | ferric citrate ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,511,536 | 1 | . | G | 92.9% | 132.3 / 3.7 | 42 | coding (879/957 nt) | fecD | ferric citrate ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base G (20/19); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑T‑GGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTA > NC_000913/4511389‑4511677 | gagTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑T‑ggg < 2:52626/151‑1 (MQ=255) aaaCGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑g‑gggggg > 2:92800/1‑148 (MQ=255) aCGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACTCCAACCGGGAGCTCC‑C‑G‑T‑ggggggatg > 2:177609/1‑146 (MQ=255) cgtTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGaataa < 2:717645/151‑1 (MQ=255) gtTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑C‑G‑TGGGGGGATGaataa > 1:776419/1‑150 (MQ=255) gTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCg < 1:499405/151‑1 (MQ=255) ccTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCca < 2:635926/151‑1 (MQ=255) gtctgtcCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑AGG‑G‑GGGGGATGAATAATTCTCGCcagc > 1:146535/1‑149 (MQ=255) gtcCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCCACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCcagc > 2:405233/1‑145 (MQ=255) ccGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCa < 2:507483/151‑1 (MQ=255) gTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCaa > 2:574829/1‑150 (MQ=255) gACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑C‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGAACGGCAAccacca > 2:804385/1‑150 (MQ=255) gACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAAccacca < 2:634660/150‑1 (MQ=255) cAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCaa < 2:59100/151‑1 (MQ=255) aGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCGCGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAAc < 2:448395/151‑1 (MQ=255) aGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAAc < 2:23217/151‑1 (MQ=255) tCAGTTCGGAAAGTGATTTATCGCATTCTCACACGCAACCAGACAAACCACGGCGCACCGATACTGGCGCTTAGCCCCCCAACCGGGGGCACC‑A‑GAG‑GGGGGGAGAACAATTCTAGGCAAGAGATCGGCAACCACCAACAGCAACGccc > 1:91446/1‑147 (MQ=255) cAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCt < 1:371165/150‑1 (MQ=255) gTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTc < 2:146558/151‑1 (MQ=255) gTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTc > 1:287863/1‑151 (MQ=255) tAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGGGCCCC‑A‑G‑TGGGGGGAAGAATAATTCTCGCCAGCAGAACGGCAACCCCCAACAGCAACGCACCTGTCAggg > 1:848289/1‑150 (MQ=255) aaaGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGc < 1:766655/150‑1 (MQ=255) aGTCATTTATCGCATTCTCACAAGCTACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGa < 1:98434/151‑1 (MQ=255) aGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGa < 1:734053/151‑1 (MQ=255) aTTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCCACCGGGAGCTCC‑C‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCCACCACCAACAGCAACGCACCTGTCAGGGCCGAAAc > 1:200914/1‑150 (MQ=255) aTTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAAc > 2:597712/1‑150 (MQ=255) cGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGG‑CGAAACAGggagc > 2:216321/1‑150 (MQ=255) aTTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGcagcag > 2:768735/1‑150 (MQ=255) cacaAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCt < 2:287888/148‑1 (MQ=255) cAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAAACGGGAGCTCCAA‑C‑G‑GGGGGGTGAATAATTGTCGCCAGCAGATTAGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCCGTCCGCGGTGACg > 2:86454/1‑151 (MQ=255) aCCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTc < 2:703278/151‑1 (MQ=255) aGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCAc > 2:588479/1‑151 (MQ=255) aGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCAc > 2:413585/1‑151 (MQ=255) aaaCCACGGCGCACCGATAATGGCGGTCAGCACGCCCACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGGCCACCGGt > 1:82359/1‑151 (MQ=255) aaaCCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGt < 1:323933/151‑1 (MQ=255) aaaCCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGGCTGCGGTGACGTCCACCgg > 1:843819/1‑150 (MQ=255) cACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATg > 2:718638/1‑151 (MQ=255) ggTCAGCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGcac < 2:646283/151‑1 (MQ=255) gCACGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACga < 2:240743/151‑1 (MQ=255) aCGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACgaga < 2:85994/151‑1 (MQ=255) aCGCCAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACg > 2:520845/1‑148 (MQ=255) cAACCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCa > 1:287885/1‑150 (MQ=255) aCCGGGAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAAt > 2:6119/1‑150 (MQ=255) ggAGCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGc > 2:786513/1‑151 (MQ=255) gCTCC‑A‑G‑TGGGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTa > 1:585936/1‑150 (MQ=255) | GAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCAACCGGGAGCTCC‑A‑G‑T‑GGGGGATGAATAATTCTCGCCAGCAGATCGGCAACCACCAACAGCAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTA > NC_000913/4511389‑4511677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |