Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091373,5810AC44.0% 80.2 / 15.0 50V314G (GTA→GGA) thiPthiamine/thiamine pyrophosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (24/4);  new base C (0/22);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.72e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTC  >  NC_000913/73432‑73730
                                                                                                                                                     |                                                                                                                                                     
tCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTa                                                                                                                                                       <  1:651274/150‑1 (MQ=255)
    ccGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCa                                                                                                                                                  <  2:546289/151‑1 (MQ=255)
        gcCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcg                                                                                                                                               >  2:551045/1‑150 (MQ=255)
        gcCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcg                                                                                                                                               >  1:597775/1‑150 (MQ=255)
        gcCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcg                                                                                                                                               >  1:564957/1‑150 (MQ=255)
          ccGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcgacg                                                                                                                                            >  2:717331/1‑151 (MQ=255)
          ccGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcgacg                                                                                                                                            <  2:698622/151‑1 (MQ=255)
          ccGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcgacg                                                                                                                                            <  1:244215/151‑1 (MQ=255)
            gCAGTTCGAGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACACTACACCTGCCGCCAGCGCAATACGCAAAGAGGTCCACAGCGCCTGCCACAGCACCGGTGGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATcgacga                                                                                                                                           >  2:621131/1‑150 (MQ=255)
             cAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGAt                                                                                                                                          <  2:338920/150‑1 (MQ=255)
                  cgcgACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGc                                                                                                                                    >  1:470500/1‑151 (MQ=255)
                        tGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTa                                                                                                                               >  2:97065/1‑150 (MQ=255)
                           tCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACgg                                                                                                                           >  1:600369/1‑151 (MQ=255)
                               tAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGgc                                                                                                                       >  1:129911/1‑151 (MQ=255)
                                  caccatcggggTAAGCCCTCCCCACAAACCCCCGGCCGCCAGCGCAATCCGAAAGGGGGCCCACGGCGCCGCCCCCAGCCCCGGTTGGGCCGCCCCTTCGGGCAACTGGCAATTTCCCCCATCGACGATCACCGCCAGTAACGGTGgcag                                                                                                                     <  1:420622/141‑1 (MQ=255)
                                          tGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAcagc                                                                                                              >  1:209993/1‑149 (MQ=255)
                                          gggccAGCACTCCGCACAATACACCGGCCGCAGGCGAAATACCAAACGAGGCCCAAAGCCCCTGCCAAAGCACCGGTTGGGCCAGCCCTCCCGGCAACGGGCGTTTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAAcagcag                                                                                                            <  2:350015/147‑1 (MQ=255)
                                           ggTCAGCACTCCGCAAAAACCCCCCGCCGCCAGCGCAATACCCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGGGCCAGCACTCCCGGCAACTGGCGTTTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAAcag                                                                                                               <  2:10049/147‑1 (MQ=255)
                                                 ccctccGCAAAATACCCCTCCCCCCAGCGCAATACGCAACGGGGCCCACAGCCCCTGCCACAGCCCCGGTTGTGCCAGCTCTTCCGGCACCTGGCGATTCCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAAcagcagcag                                                                                                         <  2:693205/142‑1 (MQ=255)
                                                   cTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGcc                                                                                                     >  2:499326/1‑149 (MQ=255)
                                                          caATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAAtt                                                                                            >  2:496180/1‑151 (MQ=255)
                                                                 ccggccgccAGCGCAATACGCAGCGGGTTCCACGGCGCCTGCCACAGCCCCGTTTGCGCCAGCATTTCCGGCACGTGGCGATTTCCCCAATCGACGATCCCCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAacac                                                                                       <  2:219558/146‑1 (MQ=255)
                                                                 cccccccccAGCACAAACCGAAACGAGGCCCCCAGCCCCTCCCACAGCCCCGTTTGCGCCAGCACTCCCGGCAACTGGCGTTTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAaca                                                                                        <  1:231659/141‑1 (MQ=255)
                                                                  cTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCgt                                                                                    >  1:310176/1‑151 (MQ=255)
                                                                          aGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCaa                                                                             >  1:809468/1‑150 (MQ=255)
                                                                               aatccacAACGAGGCCCACAGCCCCTCCCACACCCCCGTTTGGGCCAGCACTTCCGGCAACGGGCGTTTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATg                                                                          <  2:739782/142‑1 (MQ=255)
                                                                               aaTCCGCAACGAGGCCCACAGCGCGTGCCCGAGCACGGGTGGAGCAAGCGGTCCCGACCGCAGGGGATTGCCCCCGCCACCGCCAACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATg                                                                          <  2:221405/148‑1 (MQ=255)
                                                                                    gCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATg                                                                  >  1:334062/1‑151 (MQ=255)
                                                                                      aaggAGGCCCACAGCGCCTGCCACAGCCCCGGTTGGGCCAGCCCTTCCGGCAACTGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCt                                                                     <  2:13587/143‑1 (MQ=255)
                                                                                      aaCGAGGCCCCCAGCGCCTGCCACAGCGCCGGTTGGGCCAGCACTCCCGGCAACTGGCGATTCCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCa                                                                <  1:804418/151‑1 (MQ=255)
                                                                                       aCGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGc                                                                 >  2:118513/1‑149 (MQ=255)
                                                                                           ggTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACg                                                            >  1:156823/1‑150 (MQ=255)
                                                                                              ccACGGCCCCGGCCACAGCCCGGGTTGGGCCAGCACTCCGGGCAACGGGCGATTTCCCCCATCGCCAACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCg                                                        <  1:336647/151‑1 (MQ=255)
                                                                                                      ccggCCCCAGCGCCGTTTGGGCCAGTGCTTCCGGCATTTGCTGATTACCCCCAGCGAGGCCCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTc                                                      <  2:840209/142‑1 (MQ=255)
                                                                                                      ccTGCCACACCACCGGTTGTGCCAGCACTCCCGGCAACTGGCGATTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCgg                                                   <  2:812157/148‑1 (MQ=255)
                                                                                                       cTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTc                                                >  2:749014/1‑150 (MQ=255)
                                                                                                         gCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTcgcg                                             >  2:592311/1‑151 (MQ=255)
                                                                                                                cccgggtgggcccaccaCTCCCGGCAACTGGCGATTTCCCCCACCGACGACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCt                                      <  1:612764/140‑1 (MQ=255)
                                                                                                                  ccGGTTGGGCCAGCCCTTCCGGCAACTGGCGTTTTCCCCCACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTg                                    <  2:503415/151‑1 (MQ=255)
                                                                                                                    ggTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgc                                   >  2:82030/1‑150 (MQ=255)
                                                                                                                          gCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCt                                      >  2:611860/1‑141 (MQ=255)
                                                                                                                           ccaccaCTTCCGGCAACGGGCGATTTCCCCCATCGACGACCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTgcagca                               <  2:859432/147‑1 (MQ=255)
                                                                                                                            cAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtg                          >  1:230424/1‑151 (MQ=255)
                                                                                                                                cctcccGGCACCTGGCGATTCCCCCCATCGACTATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCgtggt                        <  2:750801/145‑1 (MQ=255)
                                                                                                                                    ccGGAAACTGGCGTTTTCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCggg                  <  2:334547/151‑1 (MQ=255)
                                                                                                                                     cGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGgc                 >  1:226963/1‑151 (MQ=255)
                                                                                                                                          acgggCGATTCCCCCCACCGCCGCTCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGcc                     <  1:502997/139‑1 (MQ=255)
                                                                                                                                             ggCGATTTCCCCCACCGCCGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATgg          <  2:575190/150‑1 (MQ=255)
                                                                                                                                             ggCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGc         >  2:523694/1‑151 (MQ=255)
                                                                                                                                                  tttCCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCt       <  2:226987/148‑1 (MQ=255)
                                                                                                                                                  tttCCCCCACCGACAATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCtt      <  1:190122/149‑1 (MQ=255)
                                                                                                                                                     aCCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTc  >  1:124545/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTC  >  NC_000913/73432‑73730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑