Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,903,692	0	C	A	44.3%	82.6 / 45.7	61	intergenic (+318/+1055)	ygcE/queE	putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (3/31);  new base A (27/0);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 2.57e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

TTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903545‑2903841                                                                                                                                                      |                                                                                                                                                         tttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCa                                                                                                                                                        <  2:36821/150‑1 (MQ=255) tttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCa                                                                                                                                                        <  2:618183/150‑1 (MQ=255)  ttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTTTTTT‑AAAAca                                                                                                                                                         >  1:364102/1‑148 (MQ=255)    aCAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCa                                                                                                                                                   <  1:405645/151‑1 (MQ=255)     cAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑Tc                                                                                                                                                    <  2:443365/149‑1 (MQ=255)        ggAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAg                                                                                                                                               <  2:540064/151‑1 (MQ=255)           atatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTT‑TAAAACAC‑CCAtt                                                                                                                                                 >  2:100344/1‑146 (MQ=255)             atTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCa                                                                                                                                          <  2:316633/151‑1 (MQ=255)               tAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTT‑AAAAAAAA‑ACATTAACCTCaa                                                                                                                                         >  2:273513/1‑150 (MQ=255)                     cacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑AAAAAAAA‑TCATTTGTCTCaaaaaaaa                                                                                                                                   >  2:452461/1‑150 (MQ=255)                      acTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAAACAC‑TCATTATCCTCCAaaaaaaa                                                                                                                                  >  2:202037/1‑149 (MQ=255)                        tCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑TAAAACAC‑TCATTTACCTCAAAACaaaaa                                                                                                                                 >  2:131003/1‑147 (MQ=255)                        tCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑TAAAACAC‑ACATTTATCCCAAAACaaa                                                                                                                                   >  2:148087/1‑147 (MQ=255)                          gATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAAACAA‑TCATTAGTCTCAAAACaaaaaaaa                                                                                                                              >  2:822645/1‑145 (MQ=255)                           aTGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAAc                                                                                                                           <  2:345569/151‑1 (MQ=255)                           aTGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑AATTGT‑AAAAACAC‑ACCATAAACTCaaaaaaaaaaaa                                                                                                                               >  1:843726/1‑144 (MQ=255)                                tttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑ATATGT‑AAAAACAC‑CCATTTTACTCCAAACAaaaaa‑aaaact                                                                                                                         >  2:746823/1‑145 (MQ=255)                                  tAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAAAAAA‑TCATTAAACTCaaaaaaaaaaa‑aaaa                                                                                                                           >  2:549191/1‑143 (MQ=255)                                   aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑a                                                                                                                   <  2:569057/150‑1 (MQ=255)                                     aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAAACAA‑TCAATAACATCCAAAAAAAAAA‑AAAATTCCAG‑Attt                                                                                                                >  1:617022/1‑151 (MQ=255)                                      aTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑AAAAACAC‑CCATTTTCCTCAAAAAAAAAAC‑AAAATTccccaatt                                                                                                                 >  2:293986/1‑144 (MQ=255)                                      aTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTT‑AAAAACAC‑TCATTAACCTCAAAAAAAAAAA‑AAAAATCCAG‑Aatt                                                                                                                >  2:607534/1‑150 (MQ=255)                                        aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTGT‑AAAAACAC‑CCATTAACCTCCAAAAAAAAAC‑AAAATTCCAG‑Atttttt                                                                                                             >  2:639856/1‑151 (MQ=255)                                               aaTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAAc                                                                                                      <  2:851737/151‑1 (MQ=255)                                                aTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAAcc                                                                                                     <  1:328539/151‑1 (MQ=255)                                                           aCTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACa                                                                                          <  1:768360/151‑1 (MQ=255)                                                             tttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTT‑AAAAACAC‑CCATTATCCTCCAAAAAAAAAA‑AAAATTCCCG‑AATTTTTAATTTACCAAATAATAaaaa                                                                                         >  2:812003/1‑150 (MQ=255)                                                               tATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATTT‑TAAAACAC‑TCATTTGCCTCAAAAAAAAAAC‑AAAATTCACA‑ATTTATTTCTTAAACAAAAAATAaaaa                                                                                         >  1:627505/1‑148 (MQ=255)                                                                  cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTa                                                                                    <  2:607803/150‑1 (MQ=255)                                                                  cAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTa                                                                                    <  2:442988/150‑1 (MQ=255)                                                                    aTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTaaaa                                                                                 <  2:835593/151‑1 (MQ=255)                                                                            ttAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑TAAAACAC‑ACCATTGACCCAAAAAAAAGAA‑AAACTTTCAGAATTTTTTACTT‑ACTCATTAAAAACAAAATTAAAAAAAATACt                                                                         >  1:796456/1‑151 (MQ=255)                                                                              aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑TAAAAAAC‑CCATTAGCCTCCAAAAAAAAAA‑AAAAATCCCG‑ATTTTTTTCTTTACCAAAAAAAAAAAAAATTaaaaaaaaaaatgt                                                                       >  2:417513/1‑143 (MQ=255)                                                                              aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTATCCAATGAATAACAAGCTTAAAAAATATACTg                                                                        <  1:664642/150‑1 (MQ=255)                                                                               aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGtt                                                                      <  2:628517/151‑1 (MQ=255)                                                                                aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑GAAAACAC‑CCCTTAGCTTCACAACACAAAA‑AAAATTCCAG‑ATTTTTTATTTAACCAAAGAATAAAAAAATTAAAAAATAAAccttt                                                                      >  2:567638/1‑146 (MQ=255)                                                                                aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTa                                                                     <  1:116017/151‑1 (MQ=255)                                                                                  tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAAACAC‑TCATTATCCTCAAAAAAAAAAC‑AAAAATCCAG‑ATTTTTTAATTTAACAAAGAAAAAAAAACTTAAAAAAAATAATGTTAtt                                                                   >  2:510866/1‑151 (MQ=255)                                                                                       aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑TAAAAAAC‑CCCCTTACCTCCAAACAAAAACAAAACTCCCAA‑ATTT‑TTTCTTTAACAATGAATAAAAAAATTtaaaaaaaaaattttttttt                                                                 >  2:256967/1‑134 (MQ=255)                                                                                          ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑TAAAAAAA‑ACATTAACCTCAAAAAAAAAAC‑AAAAATCCAG‑ATTTATTTTTTAAACAAAGAAAAAAAAACTTAAAAAAAATAATGTTATTTTaaaaa                                                            >  1:344305/1‑148 (MQ=255)                                                                                          ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGa                                                            <  2:665163/150‑1 (MQ=255)                                                                                              atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑TAAAAAAC‑ACATTTGACTCAAAAAAAAAAA‑AAAAATTCAG‑ATTTTTTTATTAAACAAAAAATAAAAAAATTAAAAAAAAAAATATTATTTTTAAGaaaaa                                                        >  1:389120/1‑150 (MQ=255)                                                                                              atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaaaa                                                        <  1:256942/150‑1 (MQ=255)                                                                                              atCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaaaa                                                        <  2:550324/150‑1 (MQ=255)                                                                                                    aTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTTTTT‑AAAAACAC‑TCATTAACCTCAAAAAAAAAAA‑AAAAATCCCG‑ATTTTTTACTTTAACAAAAAAAAAAAAACTTTAAAAAAAAACTGTTATTTTAAAAAAAAAAATaa                                                   >  2:89156/1‑149 (MQ=255)                                                                                                        aTAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCa                                             <  2:423466/151‑1 (MQ=255)                                                                                                          aaGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAt                                            <  2:82181/150‑1 (MQ=255)                                                                                                           aGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTa                                          <  1:815756/151‑1 (MQ=255)                                                                                                             aaCTATTCATCAACAGCGTATAGAGGCGG‑GTTTGTAAAAACCAC‑‑CAATTTCCTCAAAAAAAAAAA‑AAACCTCCCG‑ATTTATTTATTTAACAAAGAATAACAAAACTAAAAAATAAAATGTTATTCTAAAAAAAAAAATAAAAAGCAATaa                                         >  1:578558/1‑150 (MQ=255)                                                                                                              aCTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAAACACACCATTA‑CCCCAAAAAAAAAAA‑AAAAATCCAG‑ATTTTTTTCTTTAACAAATAATAAAAAACCTAAAAAAAAAAATGTTTTTTTAAAAAAAAAAATaaaaagaatt                                           >  1:111886/1‑139 (MQ=255)                                                                                                                 aTTCATCAACAGCGTATAGAGTCGG‑TTATGT‑AAAACCAC‑TCATTATCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAACAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTc                                      <  1:776286/149‑1 (MQ=255)                                                                                                                 aTTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCaa                                    <  1:724466/151‑1 (MQ=255)                                                                                                                    catcaACAGCGTATAGAGGCGG‑TTATTT‑AAAAACAC‑CCCTTAGCCCCAAAAAAAAAAA‑AAAAATCCCG‑ATTTTTTTATTTACCAAAGAAAAAAAAAATTAAAAAAAATAATGTTATTTTTAAAAAAAAAATAAAAAGCAATAATTTAata                                  >  1:236145/1‑150 (MQ=255)                                                                                                                         aCAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACATGCATTAATTCAATATTaaaa                            <  1:638474/151‑1 (MQ=255)                                                                                                                            gCGTATAGAGGCGG‑TTATGT‑AAAAACAC‑TCATTAGCCCCCAAAAAAAAAA‑AAAATTTCCG‑ATTTTTTAATTAACCAATGAAAAAAAAAATTAAAAAAAATAATGTTTTTTTTAAAAAAAAAATAAAAAGCATTAATTTAATATTtaaaaa                          >  1:712672/1‑150 (MQ=255)                                                                                                                             cGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaat                        <  1:667000/151‑1 (MQ=255)                                                                                                                                ataGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataa                      <  1:748517/150‑1 (MQ=255)                                                                                                                                    agGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataat                     >  1:95088/1‑147 (MQ=255)                                                                                                                                           ttATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAt           >  2:147609/1‑151 (MQ=255)                                                                                                                                                  aaaaCCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCtt      <  2:758578/150‑1 (MQ=255)                                                                                                                                                    aaCCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAAc   <  2:129885/151‑1 (MQ=255)                                                                                                                                                     aCCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACg  <  2:63691/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                         TTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGG‑TTATGT‑AAAACCAC‑TCATTAGCCTCAAAACAAAGAC‑AAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACG  >  NC_000913/2903545‑2903841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑