Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	203,087	T→C	G176G (GGT→GGC)	lpxA →	UDP‑N‑acetylglucosamine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	203,087	0	T	C	100.0%	175.1 / NA	55	G176G (GGT→GGC)	lpxA	UDP‑N‑acetylglucosamine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/26);  total (29/26)

GTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATCGTAG  >  NC_000913/202939‑203233                                                                                                                                                     |                                                                                                                                                   gTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGgcgt                                                                                                                                                  <  1:735232/151‑1 (MQ=255)        aggtaACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTTGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGGGgcgc                                                                                                                                             >  2:775127/1‑149 (MQ=255)        aggtaACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCa                                                                                                                                            <  1:433712/150‑1 (MQ=255)           taACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCCTGACCGCAGTCCATCAGTTCTGCATCATTGGGGCGCACGTGATGGTTGGCGGCTGCTCCGGCGGGGCGCAgg                                                                                                                                          >  2:388233/1‑149 (MQ=255)                   gTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCcctcc                                                                                                                                <  2:591074/151‑1 (MQ=255)                    tATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGTTGACTTCGCCATCATCGGCGGCATGACCGCCGTCCATCAGTTCTTCATCATTGGTGCGCCCGTGGTGGTTGGCGGCTGCTCCGGCGTGGGGCAGGACGTccc                                                                                                                                   >  1:121325/1‑147 (MQ=255)                     aTTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGGCCGCAGTCCATCAGTTCTGCATCATTTGTGCGCACGTGGTGGTTGGCGGCTGCCCCGGCGTGGCGCCGGGCGTCCCTCCtt                                                                                                                              >  1:630629/1‑151 (MQ=255)                     aTTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCcctcct                                                                                                                               <  2:669240/150‑1 (MQ=255)                     aTTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCCGCGGCATGACCGCAGTCCCTCAGTTCTGCATCATTGGAGCGCACGGGATGGTTGGCGGCTGCTCCGGCGTGGGGCAGGACGTCCCTCCtt                                                                                                                              >  1:760337/1‑151 (MQ=255)                       tctcGCCAACAACGCAACGCTGGCGGGTCACGGATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGGGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTAt                                                                                                                            >  2:35754/1‑151 (MQ=255)                          cGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTc                                                                                                                         <  1:815712/151‑1 (MQ=255)                           gCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGGTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGt                                                                                                                          >  2:699704/1‑149 (MQ=255)                             caacaaCGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCCTTTGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGGGGCGCAGGACGTCCCCCCTTATGTCAtt                                                                                                                      >  2:736961/1‑151 (MQ=255)                             caacaaCGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCAtt                                                                                                                      <  1:420224/151‑1 (MQ=255)                              aacaacGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGAACATCGGCGGCATGACCGCAGTCCATCAGTTCTTCATCCTTTGTGCGCCCGGGATGGGTGGCGGCTGCTCCGGCGGGGCGCAGGACGTCCCTCCTTATGTCAtt                                                                                                                      >  1:424142/1‑150 (MQ=255)                                               gggTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGc                                                                                                     >  2:645169/1‑150 (MQ=255)                                               gggTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGc                                                                                                     >  2:379129/1‑150 (MQ=255)                                               gggTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCa                                                                                                    <  2:308182/151‑1 (MQ=255)                                                              tGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTc                                                                                     >  1:581361/1‑151 (MQ=255)                                                               gacgacTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTc                                                                                     <  1:222480/150‑1 (MQ=255)                                                                  gacTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAat                                                                                  >  2:567546/1‑150 (MQ=255)                                                                     ttCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCg                                                                              >  2:834448/1‑151 (MQ=255)                                                                          gATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAggg                                                                         >  2:548703/1‑151 (MQ=255)                                                                           atcatcGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGc                                                                        <  1:535146/151‑1 (MQ=255)                                                                             catcGGCGGCATGACCGCAGTCAATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTg                                                                      >  2:247254/1‑151 (MQ=255)                                                                                 ggcggcATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAgc                                                                  <  2:771323/151‑1 (MQ=255)                                                                                      cATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGcgccgc                                                             >  2:460070/1‑151 (MQ=255)                                                                                        tGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCgg                                                           >  1:491202/1‑151 (MQ=255)                                                                                         gACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGa                                                          <  2:203912/151‑1 (MQ=255)                                                                                              cAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTc                                                       >  2:662738/1‑149 (MQ=255)                                                                                                gTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGcc                                                   >  1:163471/1‑151 (MQ=255)                                                                                                 tCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCg                                                  >  2:590158/1‑151 (MQ=255)                                                                                                  ccATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCg                                                  <  1:548666/150‑1 (MQ=255)                                                                                                          tCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAgc                                                                  >  2:594590/1‑126 (MQ=255)                                                                                                          tCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAgc                                                                  <  1:594551/126‑1 (MQ=255)                                                                                                           cTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGAtt                                        >  1:530408/1‑151 (MQ=255)                                                                                                             gCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGCTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTAc                                      >  1:655522/1‑151 (MQ=255)                                                                                                               atcatTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCg                                    <  1:229944/151‑1 (MQ=255)                                                                                                                 catTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCt                                  <  2:428148/151‑1 (MQ=255)                                                                                                                     ggTGCGCACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATcc                              <  1:14617/151‑1 (MQ=255)                                                                                                                         cgcACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCaa                          <  1:401789/151‑1 (MQ=255)                                                                                                                         cgcACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCaa                          <  1:303913/151‑1 (MQ=255)                                                                                                                          gcACGTGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAAt                         >  2:168344/1‑151 (MQ=255)                                                                                                                               tGATGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGAAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCg                      >  1:510759/1‑149 (MQ=255)                                                                                                                                 aTGGTTGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGtata                  <  1:168321/151‑1 (MQ=255)                                                                                                                                      tGGCGGCTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTg             >  2:687386/1‑151 (MQ=255)                                                                                                                                       ggcggcTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTg             <  1:813640/150‑1 (MQ=255)                                                                                                                                         cggcTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGAtt          >  1:562653/1‑151 (MQ=255)                                                                                                                                         cggcTGCTCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGAtt          <  2:703056/151‑1 (MQ=255)                                                                                                                                              gctCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTAt       <  2:269887/149‑1 (MQ=255)                                                                                                                                              gctCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTAt       <  1:122247/149‑1 (MQ=255)                                                                                                                                              gctCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATc      <  2:790141/150‑1 (MQ=255)                                                                                                                                                tCCGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATCGt    >  1:555118/1‑150 (MQ=255)                                                                                                                                                 ccGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCGATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATCGt    <  2:803115/149‑1 (MQ=255)                                                                                                                                                 ccGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATCGTa   <  1:531692/150‑1 (MQ=255)                                                                                                                                                 ccGGCGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATCGTAg  <  2:589354/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACGCTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAAGGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAGCTGATTTATCGTAG  >  NC_000913/202939‑203233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑